Catalytic properties of α-chymotrypsin in organic media
Adlercreutz, P. LU
and Clapes, P.
(1991)
In Biomedica Biochimica Acta
50(10-11).
p.55-60
- Abstract
α-Chymotrypsin was deposited on Celite and the resulting immobilized preparations were used to carry out peptide synthesis reactions in organic media with only small amounts of water present. The influence of different parts of the donor ester and acceptor nucleophile substrate molecules on the kinetics of the enzymatic reactions was studied. The specificity of α-chymotrypsin in organic media was a combination of its substrate specificity in aqueous media and solvent effects. The kinetics of peptide synthesis can thus be modulated by using suitable solvents and protecting groups.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/866b5b47-968a-4b93-a2a3-4029a4ef8de7
- author
- Adlercreutz, P.
LU
and Clapes, P.
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomedica Biochimica Acta
- volume
- 50
- issue
- 10-11
- pages
- 6 pages
- publisher
- Akademie Verlag
- external identifiers
-
- pmid:1820061
- scopus:0026328733
- ISSN
- 0232-766X
- language
- English
- LU publication?
- yes
- id
- 866b5b47-968a-4b93-a2a3-4029a4ef8de7
- date added to LUP
- 2019-06-22 18:43:46
- date last changed
- 2024-01-03 02:39:01
@article{866b5b47-968a-4b93-a2a3-4029a4ef8de7,
abstract = {{<p>α-Chymotrypsin was deposited on Celite and the resulting immobilized preparations were used to carry out peptide synthesis reactions in organic media with only small amounts of water present. The influence of different parts of the donor ester and acceptor nucleophile substrate molecules on the kinetics of the enzymatic reactions was studied. The specificity of α-chymotrypsin in organic media was a combination of its substrate specificity in aqueous media and solvent effects. The kinetics of peptide synthesis can thus be modulated by using suitable solvents and protecting groups.</p>}},
author = {{Adlercreutz, P. and Clapes, P.}},
issn = {{0232-766X}},
language = {{eng}},
number = {{10-11}},
pages = {{55--60}},
publisher = {{Akademie Verlag}},
series = {{Biomedica Biochimica Acta}},
title = {{Catalytic properties of α-chymotrypsin in organic media}},
volume = {{50}},
year = {{1991}},
}