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Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine

Waldie, Sarah ; Lind, Tania K LU ; Browning, Kathryn ; Moulin, Martine ; Haertlein, Michael ; Forsyth, V Trevor ; Luchini, Alessandra ; Strohmeier, Gernot A ; Pichler, Harald and Maric, Selma LU , et al. (2018) In Langmuir 34(1). p.472-479
Abstract

Cholesterol is an essential component of mammalian membranes and is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid-ordered phases with an increased thickness and a configurational order as compared to liquid-disordered phases. For saturated lipid membranes, cholesterol molecules localize close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote relocation of cholesterol toward the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the location of cholesterol (both non-deuterated and per-deuterated versions are used) within supported lipid bilayers composed of a... (More)

Cholesterol is an essential component of mammalian membranes and is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid-ordered phases with an increased thickness and a configurational order as compared to liquid-disordered phases. For saturated lipid membranes, cholesterol molecules localize close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote relocation of cholesterol toward the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the location of cholesterol (both non-deuterated and per-deuterated versions are used) within supported lipid bilayers composed of a natural mixture of phosphatidylcholine (PC). The lipids were produced in a genetically modified strain of Escherichia coli and grown under specific deuterated conditions to give an overall neutron scattering length density (which depends on the level of deuteration) of the lipids matching that of D2O. The combination of solvent contrast variation method with specific deuteration shows that cholesterol is located closer to the lipid head group-tail interface in this natural PC extract rather than in the center of the core of the bilayer as seen for very thin or polyunsaturated membranes.

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publishing date
type
Contribution to journal
publication status
published
in
Langmuir
volume
34
issue
1
pages
8 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85040308496
  • pmid:29232134
ISSN
0743-7463
DOI
10.1021/acs.langmuir.7b02716
language
English
LU publication?
no
id
86725e81-490c-4c92-801b-a5af4677152a
date added to LUP
2018-07-19 11:59:58
date last changed
2021-05-11 05:07:52
@article{86725e81-490c-4c92-801b-a5af4677152a,
  abstract     = {<p>Cholesterol is an essential component of mammalian membranes and is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid-ordered phases with an increased thickness and a configurational order as compared to liquid-disordered phases. For saturated lipid membranes, cholesterol molecules localize close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote relocation of cholesterol toward the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the location of cholesterol (both non-deuterated and per-deuterated versions are used) within supported lipid bilayers composed of a natural mixture of phosphatidylcholine (PC). The lipids were produced in a genetically modified strain of Escherichia coli and grown under specific deuterated conditions to give an overall neutron scattering length density (which depends on the level of deuteration) of the lipids matching that of D2O. The combination of solvent contrast variation method with specific deuteration shows that cholesterol is located closer to the lipid head group-tail interface in this natural PC extract rather than in the center of the core of the bilayer as seen for very thin or polyunsaturated membranes.</p>},
  author       = {Waldie, Sarah and Lind, Tania K and Browning, Kathryn and Moulin, Martine and Haertlein, Michael and Forsyth, V Trevor and Luchini, Alessandra and Strohmeier, Gernot A and Pichler, Harald and Maric, Selma and Cárdenas, Marité},
  issn         = {0743-7463},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {472--479},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Langmuir},
  title        = {Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine},
  url          = {http://dx.doi.org/10.1021/acs.langmuir.7b02716},
  doi          = {10.1021/acs.langmuir.7b02716},
  volume       = {34},
  year         = {2018},
}