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Embedding DNA in surfactant mesophases: The phase diagram of the ternary system dodecyltrimethylammonium-DNA/monoolein/water in comparison to the DNA-free analogue.

Bilalov, Azat LU ; Elsing, Jonas LU ; Haas, Eva ; Schmidt, Claudia and Olsson, Ulf LU (2013) In Journal of Colloid and Interface Science 394(Online 28 December 2012). p.360-367
Abstract
The self-assembly of a true ternary mixture comprising an electroneutral complex of DNA anions and surfactant cations (dodecyltrimethylammonium cations, DTA), water, and nonionic surfactant (monoolein, MO) has been studied. The phase diagrams of two systems, DTA-DNA/MO/water and, for comparison, dodecyltrimethylammonium bromide (DTAB)/MO/water, were obtained by visual inspection, microscopic examination under polarized light, small-angle X-ray scattering (SAXS) and deuterium NMR ((2)H NMR) at 298K and normal pressure. The isothermal phase diagram of the DTA-DNA/MO/water system contains four liquid crystalline (LC) phase regions (reversed hexagonal, Pn3m, Ia3d, lamellar). The supramolecular assemblies evolve from a bicontinuous cubic... (More)
The self-assembly of a true ternary mixture comprising an electroneutral complex of DNA anions and surfactant cations (dodecyltrimethylammonium cations, DTA), water, and nonionic surfactant (monoolein, MO) has been studied. The phase diagrams of two systems, DTA-DNA/MO/water and, for comparison, dodecyltrimethylammonium bromide (DTAB)/MO/water, were obtained by visual inspection, microscopic examination under polarized light, small-angle X-ray scattering (SAXS) and deuterium NMR ((2)H NMR) at 298K and normal pressure. The isothermal phase diagram of the DTA-DNA/MO/water system contains four liquid crystalline (LC) phase regions (reversed hexagonal, Pn3m, Ia3d, lamellar). The supramolecular assemblies evolve from a bicontinuous cubic structure of the reversed type to the two-dimensional hexagonal phase as the content of DTA-DNA is increased. While DTA-DNA tends to form a reversed hexagonal phase, DTAB is incorporated into the existing lamellar phase formed by MO and water giving rise to swelling and to significant extension of the lamellar phase region. There is only a small tendency of the cubic phases existing in the binary system MO/water to accommodate DTAB or DTA-DNA. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
394
issue
Online 28 December 2012
pages
360 - 367
publisher
Elsevier
external identifiers
  • wos:000315325400046
  • pmid:23347998
  • scopus:84873705258
  • pmid:23347998
ISSN
1095-7103
DOI
10.1016/j.jcis.2012.11.067
language
English
LU publication?
yes
id
867f7870-b6db-4d88-97e2-6bbd006a4019 (old id 3438354)
date added to LUP
2016-04-01 10:12:09
date last changed
2020-01-05 05:13:42
@article{867f7870-b6db-4d88-97e2-6bbd006a4019,
  abstract     = {The self-assembly of a true ternary mixture comprising an electroneutral complex of DNA anions and surfactant cations (dodecyltrimethylammonium cations, DTA), water, and nonionic surfactant (monoolein, MO) has been studied. The phase diagrams of two systems, DTA-DNA/MO/water and, for comparison, dodecyltrimethylammonium bromide (DTAB)/MO/water, were obtained by visual inspection, microscopic examination under polarized light, small-angle X-ray scattering (SAXS) and deuterium NMR ((2)H NMR) at 298K and normal pressure. The isothermal phase diagram of the DTA-DNA/MO/water system contains four liquid crystalline (LC) phase regions (reversed hexagonal, Pn3m, Ia3d, lamellar). The supramolecular assemblies evolve from a bicontinuous cubic structure of the reversed type to the two-dimensional hexagonal phase as the content of DTA-DNA is increased. While DTA-DNA tends to form a reversed hexagonal phase, DTAB is incorporated into the existing lamellar phase formed by MO and water giving rise to swelling and to significant extension of the lamellar phase region. There is only a small tendency of the cubic phases existing in the binary system MO/water to accommodate DTAB or DTA-DNA.},
  author       = {Bilalov, Azat and Elsing, Jonas and Haas, Eva and Schmidt, Claudia and Olsson, Ulf},
  issn         = {1095-7103},
  language     = {eng},
  number       = {Online 28 December 2012},
  pages        = {360--367},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Embedding DNA in surfactant mesophases: The phase diagram of the ternary system dodecyltrimethylammonium-DNA/monoolein/water in comparison to the DNA-free analogue.},
  url          = {http://dx.doi.org/10.1016/j.jcis.2012.11.067},
  doi          = {10.1016/j.jcis.2012.11.067},
  volume       = {394},
  year         = {2013},
}