Advanced

Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae)

Tesson, Sylvie LU ; Borra, Marco; Kooistra, Wiebe and Procaccini, Gabriele (2011) In American Journal of Botany 98(2). p.5-33
Abstract
Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction.

Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and... (More)
Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction.

Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran.

Conclusions: The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microsatellites, mutation accumulation lines, mutation rate., Mendelian inheritance, diatom, Asexual and sexual reproduction
in
American Journal of Botany
volume
98
issue
2
pages
5 - 33
publisher
Botanical Society of America
external identifiers
  • Scopus:79851504956
ISSN
0002-9122
DOI
10.3732/ajb.1000430
language
English
LU publication?
yes
id
9ef7dbcb-5c53-44f7-842d-6d318c7c5c4c (old id 8771187)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21613102
date added to LUP
2016-02-25 15:40:24
date last changed
2017-01-01 07:52:41
@article{9ef7dbcb-5c53-44f7-842d-6d318c7c5c4c,
  abstract     = {Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction. <br/><br>
Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran. <br/><br>
Conclusions: The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time.},
  author       = {Tesson, Sylvie and Borra, Marco and Kooistra, Wiebe and Procaccini, Gabriele},
  issn         = {0002-9122},
  keyword      = {microsatellites,mutation accumulation lines,mutation rate.,Mendelian inheritance,diatom,Asexual and sexual reproduction},
  language     = {eng},
  number       = {2},
  pages        = {5--33},
  publisher    = {Botanical Society of America},
  series       = {American Journal of Botany},
  title        = {Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae)},
  url          = {http://dx.doi.org/10.3732/ajb.1000430},
  volume       = {98},
  year         = {2011},
}