Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins
(1982) In Nature 298(5870). p.167-169- Abstract
Insulin-dependent diabetic (IDD) patients have a high prevalence of circulating autoantibodies against islet of Langerhans cells at the time of diagnosis1-4. Inflammatory cells within the islets5, leukocyte migration inhibition in response to pancreatic antigens6 and an association with certain HLA-D/DR histocompatibility antigens 7,8, have also been observed. It seems that the autoantibodies may be pathogenically relevant as they react primarily with β-cells9, but the specific target antigen(s) have yet to be identified. In the present study we determined whether sera from insulin-dependent diabetic children are able to immunoprecipitate proteins from detergent lysates of human... (More)
Insulin-dependent diabetic (IDD) patients have a high prevalence of circulating autoantibodies against islet of Langerhans cells at the time of diagnosis1-4. Inflammatory cells within the islets5, leukocyte migration inhibition in response to pancreatic antigens6 and an association with certain HLA-D/DR histocompatibility antigens 7,8, have also been observed. It seems that the autoantibodies may be pathogenically relevant as they react primarily with β-cells9, but the specific target antigen(s) have yet to be identified. In the present study we determined whether sera from insulin-dependent diabetic children are able to immunoprecipitate proteins from detergent lysates of human islet cells. We report that sera from 8 out of 10 newly diagnosed diabetic children consistently immunoprecipitate a protein having a molecular weight (M r) of ∼64,000 (64K). An additional protein (38K) was precipitated from islet cells obtained from a HLA-DR3-positive donor. Neither of the proteins was precipitated by non-diabetic sera nor detected in immunoprecipitates from human lymphocyte lysates. It is suggested that the 64K and/or 38K protein components may represent cell-specific target antigens in insulin-dependent diabetes.
(Less)
- author
- Baekkeskov, Steinunn ; Nielsen, Jens Høiriis ; Marner, Birgitte ; Bilde, Torben ; Ludvigsson, Johnny and Lernmark, Ake LU
- publishing date
- 1982-12-01
- type
- Contribution to journal
- publication status
- published
- in
- Nature
- volume
- 298
- issue
- 5870
- pages
- 3 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:7045690
- scopus:0019959221
- ISSN
- 0028-0836
- DOI
- 10.1038/298167a0
- language
- English
- LU publication?
- no
- id
- 877bb43c-b628-4848-a290-6d97fcc3e64e
- date added to LUP
- 2019-09-16 15:32:53
- date last changed
- 2024-03-13 08:14:06
@article{877bb43c-b628-4848-a290-6d97fcc3e64e, abstract = {{<p>Insulin-dependent diabetic (IDD) patients have a high prevalence of circulating autoantibodies against islet of Langerhans cells at the time of diagnosis<sup>1-4</sup>. Inflammatory cells within the islets<sup>5</sup>, leukocyte migration inhibition in response to pancreatic antigens<sup>6</sup> and an association with certain HLA-D/DR histocompatibility antigens <sup>7,8</sup>, have also been observed. It seems that the autoantibodies may be pathogenically relevant as they react primarily with β-cells<sup>9</sup>, but the specific target antigen(s) have yet to be identified. In the present study we determined whether sera from insulin-dependent diabetic children are able to immunoprecipitate proteins from detergent lysates of human islet cells. We report that sera from 8 out of 10 newly diagnosed diabetic children consistently immunoprecipitate a protein having a molecular weight (M <sub>r</sub>) of ∼64,000 (64K). An additional protein (38K) was precipitated from islet cells obtained from a HLA-DR3-positive donor. Neither of the proteins was precipitated by non-diabetic sera nor detected in immunoprecipitates from human lymphocyte lysates. It is suggested that the 64K and/or 38K protein components may represent cell-specific target antigens in insulin-dependent diabetes.</p>}}, author = {{Baekkeskov, Steinunn and Nielsen, Jens Høiriis and Marner, Birgitte and Bilde, Torben and Ludvigsson, Johnny and Lernmark, Ake}}, issn = {{0028-0836}}, language = {{eng}}, month = {{12}}, number = {{5870}}, pages = {{167--169}}, publisher = {{Nature Publishing Group}}, series = {{Nature}}, title = {{Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins}}, url = {{http://dx.doi.org/10.1038/298167a0}}, doi = {{10.1038/298167a0}}, volume = {{298}}, year = {{1982}}, }