Mast Cell Proteases Promote Diverse Effects on the Plasminogen Activation System and Wound Healing in A549 Alveolar Epithelial Cells
(2022) In Cells 11(18).- Abstract
Tissue damage, epithelial alterations, and intraepithelial presence of mast cells (MCs) are characteristics of asthma pathogenesis. Increased alveolar infiltration of MC populations has also been identified as a feature of asthma and other chronic respiratory diseases. The asthma associated receptor, urokinase plasminogen activator receptor (uPAR), has been shown to regulate bronchial epithelial repair responses. However, the impact of MC tryptase and chymase on functional properties and expression of uPAR in alveolar epithelial cells have not been fully investigated. Alveolar epithelial cell migration and wound healing were investigated using holographic live cell imaging of A549 cells in a wound scratch model post stimulation with... (More)
Tissue damage, epithelial alterations, and intraepithelial presence of mast cells (MCs) are characteristics of asthma pathogenesis. Increased alveolar infiltration of MC populations has also been identified as a feature of asthma and other chronic respiratory diseases. The asthma associated receptor, urokinase plasminogen activator receptor (uPAR), has been shown to regulate bronchial epithelial repair responses. However, the impact of MC tryptase and chymase on functional properties and expression of uPAR in alveolar epithelial cells have not been fully investigated. Alveolar epithelial cell migration and wound healing were investigated using holographic live cell imaging of A549 cells in a wound scratch model post stimulation with tryptase or chymase. The expression of uPAR was investigated on the protein and gene level from cellular supernatants and in bronchoalveolar lavage fluid fractions from allergic asthmatics. We found that tryptase improved wound healing capacity, cellular migration and membrane bound uPAR expression. Chymase reduced gap closure capacity, cellular migration and membrane bound uPAR expression but increased soluble uPAR release. Our data suggest a dual regulatory response from the MC proteases in events related to uPAR expression and wound healing which could be important features in asthmatic disease.
(Less)
- author
- Mogren, Sofia
LU
; Berlin, Frida
LU
; Eskilsson, Lykke
; Van Der Burg, Nicole
LU
; Tufvesson, Ellen LU and Andersson, Cecilia K. LU
- organization
- publishing date
- 2022-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- alveolar epithelial cells, chymase, mast cell, migration, tryptase, urokinase plasminogen activator receptor, wound healing
- in
- Cells
- volume
- 11
- issue
- 18
- article number
- 2916
- publisher
- MDPI AG
- external identifiers
-
- scopus:85138665769
- pmid:36139491
- ISSN
- 2073-4409
- DOI
- 10.3390/cells11182916
- language
- English
- LU publication?
- yes
- id
- 87a5dd9d-e71e-4243-bcf7-d9710f6c2997
- date added to LUP
- 2022-12-12 11:40:32
- date last changed
- 2025-01-11 19:04:55
@article{87a5dd9d-e71e-4243-bcf7-d9710f6c2997, abstract = {{<p>Tissue damage, epithelial alterations, and intraepithelial presence of mast cells (MCs) are characteristics of asthma pathogenesis. Increased alveolar infiltration of MC populations has also been identified as a feature of asthma and other chronic respiratory diseases. The asthma associated receptor, urokinase plasminogen activator receptor (uPAR), has been shown to regulate bronchial epithelial repair responses. However, the impact of MC tryptase and chymase on functional properties and expression of uPAR in alveolar epithelial cells have not been fully investigated. Alveolar epithelial cell migration and wound healing were investigated using holographic live cell imaging of A549 cells in a wound scratch model post stimulation with tryptase or chymase. The expression of uPAR was investigated on the protein and gene level from cellular supernatants and in bronchoalveolar lavage fluid fractions from allergic asthmatics. We found that tryptase improved wound healing capacity, cellular migration and membrane bound uPAR expression. Chymase reduced gap closure capacity, cellular migration and membrane bound uPAR expression but increased soluble uPAR release. Our data suggest a dual regulatory response from the MC proteases in events related to uPAR expression and wound healing which could be important features in asthmatic disease.</p>}}, author = {{Mogren, Sofia and Berlin, Frida and Eskilsson, Lykke and Van Der Burg, Nicole and Tufvesson, Ellen and Andersson, Cecilia K.}}, issn = {{2073-4409}}, keywords = {{alveolar epithelial cells; chymase; mast cell; migration; tryptase; urokinase plasminogen activator receptor; wound healing}}, language = {{eng}}, number = {{18}}, publisher = {{MDPI AG}}, series = {{Cells}}, title = {{Mast Cell Proteases Promote Diverse Effects on the Plasminogen Activation System and Wound Healing in A549 Alveolar Epithelial Cells}}, url = {{http://dx.doi.org/10.3390/cells11182916}}, doi = {{10.3390/cells11182916}}, volume = {{11}}, year = {{2022}}, }