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Small-Angle X-ray Scattering Demonstrates Similar Nanostructure in Cortical Bone from Young Adult Animals of Different Species.

Døvling Kaspersen, Jørn LU ; Turunen, Mikael LU ; Mathavan, Neashan LU ; Lages, Sebastian LU ; Pedersen, Jan Skov; Olsson, Ulf LU and Isaksson, Hanna LU (2016) In Calcified Tissue International
Abstract
Despite the vast amount of studies focusing on bone nanostructure that have been performed for several decades, doubts regarding the detailed structure of the constituting hydroxyapatite crystal still exist. Different experimental techniques report somewhat different sizes and locations, possibly due to different requirements for the sample preparation. In this study, small- and wide-angle X-ray scattering is used to investigate the nanostructure of femur samples from young adult ovine, bovine, porcine, and murine cortical bone, including three different orthogonal directions relative to the long axis of the bone. The radially averaged scattering from all samples reveals a remarkable similarity in the entire q range, which indicates that... (More)
Despite the vast amount of studies focusing on bone nanostructure that have been performed for several decades, doubts regarding the detailed structure of the constituting hydroxyapatite crystal still exist. Different experimental techniques report somewhat different sizes and locations, possibly due to different requirements for the sample preparation. In this study, small- and wide-angle X-ray scattering is used to investigate the nanostructure of femur samples from young adult ovine, bovine, porcine, and murine cortical bone, including three different orthogonal directions relative to the long axis of the bone. The radially averaged scattering from all samples reveals a remarkable similarity in the entire q range, which indicates that the nanostructure is essentially the same in all species. Small differences in the data from different directions confirm that the crystals are elongated in the [001] direction and that this direction is parallel to the long axis of the bone. A model consisting of thin plates is successfully employed to describe the scattering and extract the plate thicknesses, which are found to be in the range of 20-40 Å for most samples but 40-60 Å for the cow samples. It is demonstrated that the mineral plates have a large degree of polydispersity in plate thickness. Additionally, and equally importantly, the scattering data and the model are critically evaluated in terms of model uncertainties and overall information content. (Less)
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Contribution to journal
publication status
published
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in
Calcified Tissue International
publisher
Springer
external identifiers
  • pmid:26914607
  • scopus:84971318144
  • wos:000377012800008
ISSN
1432-0827
DOI
10.1007/s00223-016-0120-z
language
English
LU publication?
yes
id
1ab9ed9c-a844-4d62-997f-1351e1f70837 (old id 8821669)
date added to LUP
2016-03-11 11:29:39
date last changed
2017-02-19 03:54:52
@article{1ab9ed9c-a844-4d62-997f-1351e1f70837,
  abstract     = {Despite the vast amount of studies focusing on bone nanostructure that have been performed for several decades, doubts regarding the detailed structure of the constituting hydroxyapatite crystal still exist. Different experimental techniques report somewhat different sizes and locations, possibly due to different requirements for the sample preparation. In this study, small- and wide-angle X-ray scattering is used to investigate the nanostructure of femur samples from young adult ovine, bovine, porcine, and murine cortical bone, including three different orthogonal directions relative to the long axis of the bone. The radially averaged scattering from all samples reveals a remarkable similarity in the entire q range, which indicates that the nanostructure is essentially the same in all species. Small differences in the data from different directions confirm that the crystals are elongated in the [001] direction and that this direction is parallel to the long axis of the bone. A model consisting of thin plates is successfully employed to describe the scattering and extract the plate thicknesses, which are found to be in the range of 20-40 Å for most samples but 40-60 Å for the cow samples. It is demonstrated that the mineral plates have a large degree of polydispersity in plate thickness. Additionally, and equally importantly, the scattering data and the model are critically evaluated in terms of model uncertainties and overall information content.},
  author       = {Døvling Kaspersen, Jørn and Turunen, Mikael and Mathavan, Neashan and Lages, Sebastian and Pedersen, Jan Skov and Olsson, Ulf and Isaksson, Hanna},
  issn         = {1432-0827},
  language     = {eng},
  month        = {02},
  publisher    = {Springer},
  series       = {Calcified Tissue International},
  title        = {Small-Angle X-ray Scattering Demonstrates Similar Nanostructure in Cortical Bone from Young Adult Animals of Different Species.},
  url          = {http://dx.doi.org/10.1007/s00223-016-0120-z},
  year         = {2016},
}