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Quasi-Continuous Wave Near-Infrared Excitation of Upconversion Nanoparticles for Optogenetic Manipulation of C. elegans.

Bansal, Akshaya; Liu, Haichun; Jayakumar, Muthu Kumara Gnanasammandhan; Andersson-Engels, Stefan LU and Zhang, Yong (2016) In Small 12(13). p.1732-1743
Abstract
Optogenetics is an emerging powerful tool to investigate workings of the nervous system. However, the use of low tissue penetrating visible light limits its therapeutic potential. Employing deep penetrating near-infrared (NIR) light for optogenetics would be beneficial but it cannot be used directly. This issue can be tackled with upconversion nanoparticles (UCNs) acting as nanotransducers emitting at shorter wavelengths extending to the UV range upon NIR light excitation. Although attractive, implementation of such NIR-optogenetics is hindered by the low UCN emission intensity that necessitates high NIR excitation intensities, resulting in overheating issues. A novel quasi-continuous wave (quasi-CW) excitation approach is developed that... (More)
Optogenetics is an emerging powerful tool to investigate workings of the nervous system. However, the use of low tissue penetrating visible light limits its therapeutic potential. Employing deep penetrating near-infrared (NIR) light for optogenetics would be beneficial but it cannot be used directly. This issue can be tackled with upconversion nanoparticles (UCNs) acting as nanotransducers emitting at shorter wavelengths extending to the UV range upon NIR light excitation. Although attractive, implementation of such NIR-optogenetics is hindered by the low UCN emission intensity that necessitates high NIR excitation intensities, resulting in overheating issues. A novel quasi-continuous wave (quasi-CW) excitation approach is developed that significantly enhances multiphoton emissions from UCNs, and for the first time NIR light-triggered optogenetic manipulations are implemented in vitro and in C. elegans. The approach developed here enables the activation of channelrhodopsin-2 with a significantly lower excitation power and UCN concentration along with negligible phototoxicity as seen with CW excitation, paving the way for therapeutic optogenetics. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Small
volume
12
issue
13
pages
1732 - 1743
publisher
John Wiley & Sons
external identifiers
  • pmid:26849846
  • scopus:84958998826
  • wos:000373733200009
ISSN
1613-6829
DOI
10.1002/smll.201503792
language
English
LU publication?
yes
id
29e9d1bd-95c3-4b6c-ace5-3eb5f1c45902 (old id 8829165)
date added to LUP
2016-03-04 09:41:59
date last changed
2017-02-08 12:39:21
@article{29e9d1bd-95c3-4b6c-ace5-3eb5f1c45902,
  abstract     = {Optogenetics is an emerging powerful tool to investigate workings of the nervous system. However, the use of low tissue penetrating visible light limits its therapeutic potential. Employing deep penetrating near-infrared (NIR) light for optogenetics would be beneficial but it cannot be used directly. This issue can be tackled with upconversion nanoparticles (UCNs) acting as nanotransducers emitting at shorter wavelengths extending to the UV range upon NIR light excitation. Although attractive, implementation of such NIR-optogenetics is hindered by the low UCN emission intensity that necessitates high NIR excitation intensities, resulting in overheating issues. A novel quasi-continuous wave (quasi-CW) excitation approach is developed that significantly enhances multiphoton emissions from UCNs, and for the first time NIR light-triggered optogenetic manipulations are implemented in vitro and in C. elegans. The approach developed here enables the activation of channelrhodopsin-2 with a significantly lower excitation power and UCN concentration along with negligible phototoxicity as seen with CW excitation, paving the way for therapeutic optogenetics.},
  author       = {Bansal, Akshaya and Liu, Haichun and Jayakumar, Muthu Kumara Gnanasammandhan and Andersson-Engels, Stefan and Zhang, Yong},
  issn         = {1613-6829},
  language     = {eng},
  month        = {02},
  number       = {13},
  pages        = {1732--1743},
  publisher    = {John Wiley & Sons},
  series       = {Small},
  title        = {Quasi-Continuous Wave Near-Infrared Excitation of Upconversion Nanoparticles for Optogenetic Manipulation of C. elegans.},
  url          = {http://dx.doi.org/10.1002/smll.201503792},
  volume       = {12},
  year         = {2016},
}