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A population-based study of aerococcal bacteraemia in the MALDI-TOF MS-era.

Senneby, Erik LU ; Göransson, L; Weiber, S and Rasmussen, Magnus LU (2016) In European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 35(5). p.755-762
Abstract
The purpose of this study was to determine the incidence of aerococcal bacteraemia in the MALDI-TOF MS-era, to describe the clinical presentation and to determine the MIC values of aerococci for ten antibiotics. Aerococci in blood cultures were identified through searches in the laboratory database for the years 2012-2014. MALDI-TOF MS, sequencing of the 16S rRNA gene and a PYR test were used for species identification. Patients' medical charts were systematically reviewed. Etests were used to determine MIC values. Seventy-seven patients were identified (Aerococcus urinae n = 49, Aerococcus viridans n = 14, Aerococcus sanguinicola n = 13 and Aerococcus christensenii n = 1) corresponding to incidences of 14 cases per 1,000,000 inhabitants... (More)
The purpose of this study was to determine the incidence of aerococcal bacteraemia in the MALDI-TOF MS-era, to describe the clinical presentation and to determine the MIC values of aerococci for ten antibiotics. Aerococci in blood cultures were identified through searches in the laboratory database for the years 2012-2014. MALDI-TOF MS, sequencing of the 16S rRNA gene and a PYR test were used for species identification. Patients' medical charts were systematically reviewed. Etests were used to determine MIC values. Seventy-seven patients were identified (Aerococcus urinae n = 49, Aerococcus viridans n = 14, Aerococcus sanguinicola n = 13 and Aerococcus christensenii n = 1) corresponding to incidences of 14 cases per 1,000,000 inhabitants per year (A. urinae) and 3.5 cases per 1,000,000 inhabitants per year (A. sanguinicola and A.viridans). A. urinae was in pure culture in 61 %, A. sanguinicola in 46 % and A. viridans in 36 % of the cases. The A. urinae and A. sanguinicola patients were old and many had urinary tract disorders, and a majority had a suspected urinary tract focus of the bacteraemia. Eighty percent of the A. urinae patients were men. Five A. urinae patients were diagnosed with infective endocarditis. Six patients died within 30 days. Most isolates had low MICs to penicillins and carbapenems. MALDI-TOF MS has led to an increased identification of aerococcal bacteremia. A. urinae remains the most common Aerococcus in blood cultures and in aerococcal IE. (Less)
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Contribution to journal
publication status
published
subject
in
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
volume
35
issue
5
pages
755 - 762
publisher
Springer
external identifiers
  • pmid:26838685
  • scopus:84964286187
  • wos:000374568800005
ISSN
1435-4373
DOI
10.1007/s10096-016-2594-z
language
English
LU publication?
yes
id
2aac15ab-2f6a-4028-b52e-48134ac34a26 (old id 8829433)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26838685?dopt=Abstract
date added to LUP
2016-03-01 13:21:33
date last changed
2017-11-19 03:56:44
@article{2aac15ab-2f6a-4028-b52e-48134ac34a26,
  abstract     = {The purpose of this study was to determine the incidence of aerococcal bacteraemia in the MALDI-TOF MS-era, to describe the clinical presentation and to determine the MIC values of aerococci for ten antibiotics. Aerococci in blood cultures were identified through searches in the laboratory database for the years 2012-2014. MALDI-TOF MS, sequencing of the 16S rRNA gene and a PYR test were used for species identification. Patients' medical charts were systematically reviewed. Etests were used to determine MIC values. Seventy-seven patients were identified (Aerococcus urinae n = 49, Aerococcus viridans n = 14, Aerococcus sanguinicola n = 13 and Aerococcus christensenii n = 1) corresponding to incidences of 14 cases per 1,000,000 inhabitants per year (A. urinae) and 3.5 cases per 1,000,000 inhabitants per year (A. sanguinicola and A.viridans). A. urinae was in pure culture in 61 %, A. sanguinicola in 46 % and A. viridans in 36 % of the cases. The A. urinae and A. sanguinicola patients were old and many had urinary tract disorders, and a majority had a suspected urinary tract focus of the bacteraemia. Eighty percent of the A. urinae patients were men. Five A. urinae patients were diagnosed with infective endocarditis. Six patients died within 30 days. Most isolates had low MICs to penicillins and carbapenems. MALDI-TOF MS has led to an increased identification of aerococcal bacteremia. A. urinae remains the most common Aerococcus in blood cultures and in aerococcal IE.},
  author       = {Senneby, Erik and Göransson, L and Weiber, S and Rasmussen, Magnus},
  issn         = {1435-4373},
  language     = {eng},
  number       = {5},
  pages        = {755--762},
  publisher    = {Springer},
  series       = {European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology},
  title        = {A population-based study of aerococcal bacteraemia in the MALDI-TOF MS-era.},
  url          = {http://dx.doi.org/10.1007/s10096-016-2594-z},
  volume       = {35},
  year         = {2016},
}