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Optimization of galactooligosaccharide production from lactose using β-glycosidases from hyperthermophiles

Hansson, Therese LU and Adlercreutz, Patrick LU (2001) In Food Biotechnology 15(2). p.79-97
Abstract

The maximal production of galactooligosaccharides (GOS) from lactose by (β-glycosidases from the hyperthermophilic archaea, Sulfolobus solfataricus (LacS) (derived from lacS gene) and Pyrococcus furiosus (CelB) (derived from celB gene) was optimized. The performance of these enzymes under extreme reaction conditions, temperatures up to 95°C and lactose concentrations up to 90% (w/v), were studied. The highly thermostable enzymes were shown to be very well suited for oligosaccharide synthesis. For both LacS and CelB the maximum yield of GOS increased with increasing lactose concentration, up to 70% (w/v). The maximum yield of GOS also increased with increasing temperature, and the optimal pH for synthesis was different at different... (More)

The maximal production of galactooligosaccharides (GOS) from lactose by (β-glycosidases from the hyperthermophilic archaea, Sulfolobus solfataricus (LacS) (derived from lacS gene) and Pyrococcus furiosus (CelB) (derived from celB gene) was optimized. The performance of these enzymes under extreme reaction conditions, temperatures up to 95°C and lactose concentrations up to 90% (w/v), were studied. The highly thermostable enzymes were shown to be very well suited for oligosaccharide synthesis. For both LacS and CelB the maximum yield of GOS increased with increasing lactose concentration, up to 70% (w/v). The maximum yield of GOS also increased with increasing temperature, and the optimal pH for synthesis was different at different temperatures. The sum of tri- and tetrasaccharides yielded 37% (w/w) in an optimally designed reaction for LacS, and a maximum yield of 40% (w/w) was attained for CelB. Compared to aqueous solution, an increase of the tetrasaccharide/trisaccharide ratio was obtained in two-phase systems with heptane and nonane. These two enzymes from hyperthermophilic organisms were shown to give higher GOS yields at high substrate concentrations than a β-galactosidase from a thermophilic/mesophilic organism (Aspergillus oryzae).

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type
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publication status
published
subject
in
Food Biotechnology
volume
15
issue
2
pages
19 pages
publisher
Taylor & Francis
external identifiers
  • scopus:0035715016
ISSN
0890-5436
DOI
10.1081/FBT-100106830
language
English
LU publication?
yes
id
88350490-2642-40fe-af3b-df9f120fdedc
date added to LUP
2019-06-20 15:48:52
date last changed
2020-08-26 04:53:36
@article{88350490-2642-40fe-af3b-df9f120fdedc,
  abstract     = {<p>The maximal production of galactooligosaccharides (GOS) from lactose by (β-glycosidases from the hyperthermophilic archaea, Sulfolobus solfataricus (LacS) (derived from lacS gene) and Pyrococcus furiosus (CelB) (derived from celB gene) was optimized. The performance of these enzymes under extreme reaction conditions, temperatures up to 95°C and lactose concentrations up to 90% (w/v), were studied. The highly thermostable enzymes were shown to be very well suited for oligosaccharide synthesis. For both LacS and CelB the maximum yield of GOS increased with increasing lactose concentration, up to 70% (w/v). The maximum yield of GOS also increased with increasing temperature, and the optimal pH for synthesis was different at different temperatures. The sum of tri- and tetrasaccharides yielded 37% (w/w) in an optimally designed reaction for LacS, and a maximum yield of 40% (w/w) was attained for CelB. Compared to aqueous solution, an increase of the tetrasaccharide/trisaccharide ratio was obtained in two-phase systems with heptane and nonane. These two enzymes from hyperthermophilic organisms were shown to give higher GOS yields at high substrate concentrations than a β-galactosidase from a thermophilic/mesophilic organism (Aspergillus oryzae).</p>},
  author       = {Hansson, Therese and Adlercreutz, Patrick},
  issn         = {0890-5436},
  language     = {eng},
  month        = {12},
  number       = {2},
  pages        = {79--97},
  publisher    = {Taylor & Francis},
  series       = {Food Biotechnology},
  title        = {Optimization of galactooligosaccharide production from lactose using β-glycosidases from hyperthermophiles},
  url          = {http://dx.doi.org/10.1081/FBT-100106830},
  doi          = {10.1081/FBT-100106830},
  volume       = {15},
  year         = {2001},
}