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Long‑term follow‑up of buserelin‑induced enteric neuropathy in rats.

Jönsson, Anette LU ; Sand, Elin LU ; Ekblad, Eva LU and Ohlsson, Bodil LU (2016) In Molecular Medicine Reports 13(4). p.3507-3513
Abstract
A few patients have been shown to develop severe abdominal pain and gastrointestinal dysmotility during treatment with gonadotropin‑releasing hormone (GnRH) analogs. A rat model of enteric neuropathy has been developed by administration of the GnRH analog buserelin to rats. Loss of enteric neurons and ganglioneuritis throughout the gastrointestinal tract has been described, without other histopathological changes. The aim of the present study was to investigate the long‑term effects of this rat model on body weight, and on morphology and inflammatory changes in the gastrointestinal tract. Rats were administered subcutaneous injections of buserelin or saline once daily for 5 days and allowed to recover for 3 weeks. This regimen was repeated... (More)
A few patients have been shown to develop severe abdominal pain and gastrointestinal dysmotility during treatment with gonadotropin‑releasing hormone (GnRH) analogs. A rat model of enteric neuropathy has been developed by administration of the GnRH analog buserelin to rats. Loss of enteric neurons and ganglioneuritis throughout the gastrointestinal tract has been described, without other histopathological changes. The aim of the present study was to investigate the long‑term effects of this rat model on body weight, and on morphology and inflammatory changes in the gastrointestinal tract. Rats were administered subcutaneous injections of buserelin or saline once daily for 5 days and allowed to recover for 3 weeks. This regimen was repeated four times. The rats were weighed weekly and were sacrificed 16 weeks after the fourth treatment. The bowel wall was measured by morphometry, and the presence of enteric neurons, mast cells, eosinophils and T‑lymphocytes was evaluated. Buserelin‑treated rats were shown to have a lower body weight at sacrifice, as compared with the controls (P<0.05). Compared with controls, buserelin treatment caused loss of myenteric neurons in the ileum and colon (P<0.01), a thinner circular muscle layer in ileum (P<0.05) and longitudinal muscle layer in colon (P<0.05), increased number of eosinophils in the submucosa of the ileum (P<0.05), and an increased number of T‑lymphocytes in the submucosa and circular muscle layer of the fundus (P<0.01 and P<0.05, respectively) and circular muscle layer of the colon (P<0.05). Mast cells were equally distributed in the two groups. Thus, long‑term follow‑up of buserelin‑induced enteric neuropathy reveals reduced body weight, loss of myenteric neurons, thinning of muscle layers, and increased numbers of eosinophils and T‑lymphocytes in the gastrointestinal tract. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Medicine Reports
volume
13
issue
4
pages
3507 - 3513
publisher
Spandidos Publications Ltd
external identifiers
  • pmid:26935850
  • wos:000373582100074
  • scopus:84963827961
ISSN
1791-3004
DOI
10.3892/mmr.2016.4968
language
English
LU publication?
yes
id
1aea5e75-3945-40ba-bcf6-4ea18bcba93d (old id 8856458)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26935850?dopt=Abstract
date added to LUP
2016-03-15 11:44:53
date last changed
2017-01-01 07:32:02
@article{1aea5e75-3945-40ba-bcf6-4ea18bcba93d,
  abstract     = {A few patients have been shown to develop severe abdominal pain and gastrointestinal dysmotility during treatment with gonadotropin‑releasing hormone (GnRH) analogs. A rat model of enteric neuropathy has been developed by administration of the GnRH analog buserelin to rats. Loss of enteric neurons and ganglioneuritis throughout the gastrointestinal tract has been described, without other histopathological changes. The aim of the present study was to investigate the long‑term effects of this rat model on body weight, and on morphology and inflammatory changes in the gastrointestinal tract. Rats were administered subcutaneous injections of buserelin or saline once daily for 5 days and allowed to recover for 3 weeks. This regimen was repeated four times. The rats were weighed weekly and were sacrificed 16 weeks after the fourth treatment. The bowel wall was measured by morphometry, and the presence of enteric neurons, mast cells, eosinophils and T‑lymphocytes was evaluated. Buserelin‑treated rats were shown to have a lower body weight at sacrifice, as compared with the controls (P&lt;0.05). Compared with controls, buserelin treatment caused loss of myenteric neurons in the ileum and colon (P&lt;0.01), a thinner circular muscle layer in ileum (P&lt;0.05) and longitudinal muscle layer in colon (P&lt;0.05), increased number of eosinophils in the submucosa of the ileum (P&lt;0.05), and an increased number of T‑lymphocytes in the submucosa and circular muscle layer of the fundus (P&lt;0.01 and P&lt;0.05, respectively) and circular muscle layer of the colon (P&lt;0.05). Mast cells were equally distributed in the two groups. Thus, long‑term follow‑up of buserelin‑induced enteric neuropathy reveals reduced body weight, loss of myenteric neurons, thinning of muscle layers, and increased numbers of eosinophils and T‑lymphocytes in the gastrointestinal tract.},
  author       = {Jönsson, Anette and Sand, Elin and Ekblad, Eva and Ohlsson, Bodil},
  issn         = {1791-3004},
  language     = {eng},
  month        = {03},
  number       = {4},
  pages        = {3507--3513},
  publisher    = {Spandidos Publications Ltd},
  series       = {Molecular Medicine Reports},
  title        = {Long‑term follow‑up of buserelin‑induced enteric neuropathy in rats.},
  url          = {http://dx.doi.org/10.3892/mmr.2016.4968},
  volume       = {13},
  year         = {2016},
}