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Modulation of antibiotic resistance and induction of a stress response in Pseudomonas aeruginosa by silver nanoparticles

Markowska, Katarzyna; Grudniak, Anna M.; Krawczyk, Krzysztof LU ; Wróbel, Izabela and Wolska, Krystyna I. (2014) In Journal of Medical Microbiology 63(6). p.849-854
Abstract
The objective of this study was to characterize the effects of silver nanoparticles on Pseudomonas aeruginosa. Their interactions with several conventional antibiotics and ability to induce a stress response were examined. Interactions between silver nanoparticles (AgNPs) and antibiotics against free-living cells and biofilm of P. aeruginosa were studied using the chequerboard method and time-kill assays. The ability of AgNPs to induce a stress response was determined by evaluation of cellular levels of the DnaK and HtpG chaperones using SDS-PAGE and Western blot analysis. Synergistic activity against free-living P. aeruginosa between AgNPs and ampicillin, streptomycin, rifampicin and tetracycline, but not oxacillin, ciprofloxacin,... (More)
The objective of this study was to characterize the effects of silver nanoparticles on Pseudomonas aeruginosa. Their interactions with several conventional antibiotics and ability to induce a stress response were examined. Interactions between silver nanoparticles (AgNPs) and antibiotics against free-living cells and biofilm of P. aeruginosa were studied using the chequerboard method and time-kill assays. The ability of AgNPs to induce a stress response was determined by evaluation of cellular levels of the DnaK and HtpG chaperones using SDS-PAGE and Western blot analysis. Synergistic activity against free-living P. aeruginosa between AgNPs and ampicillin, streptomycin, rifampicin and tetracycline, but not oxacillin, ciprofloxacin, meropenem or ceftazidime, was demonstrated by the chequerboard method. No such interactions were observed against P. aeruginosa biofilm. The results of time-kill assays confirmed synergy only for the AgNPs–streptomycin combination. AgNPs induced the expression of chaperone DnaK. No induction of the HtpG chaperone was detected. In conclusion, AgNPs not only display potent bactericidal activity against P. aeruginosa, but also act synergistically with several conventional antibiotics to enhance their effect against free-living bacteria as determined by the chequerboard method. The time-kill assay proved synergy between AgNPs and streptomycin only. The ability of AgNPs to induce the major chaperone protein DnaK may influence bacterial resistance to antimicrobials. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Medical Microbiology
volume
63
issue
6
pages
849 - 854
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:84901322922
ISSN
0022-2615
DOI
10.1099/jmm.0.068833-0
language
English
LU publication?
no
id
886bfcf8-59b8-4f2d-9465-148b21cb748b
alternative location
http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.068833-0#tab2
date added to LUP
2018-08-15 08:48:52
date last changed
2018-09-16 04:56:37
@article{886bfcf8-59b8-4f2d-9465-148b21cb748b,
  abstract     = {The objective of this study was to characterize the effects of silver nanoparticles on Pseudomonas aeruginosa. Their interactions with several conventional antibiotics and ability to induce a stress response were examined. Interactions between silver nanoparticles (AgNPs) and antibiotics against free-living cells and biofilm of P. aeruginosa were studied using the chequerboard method and time-kill assays. The ability of AgNPs to induce a stress response was determined by evaluation of cellular levels of the DnaK and HtpG chaperones using SDS-PAGE and Western blot analysis. Synergistic activity against free-living P. aeruginosa between AgNPs and ampicillin, streptomycin, rifampicin and tetracycline, but not oxacillin, ciprofloxacin, meropenem or ceftazidime, was demonstrated by the chequerboard method. No such interactions were observed against P. aeruginosa biofilm. The results of time-kill assays confirmed synergy only for the AgNPs–streptomycin combination. AgNPs induced the expression of chaperone DnaK. No induction of the HtpG chaperone was detected. In conclusion, AgNPs not only display potent bactericidal activity against P. aeruginosa, but also act synergistically with several conventional antibiotics to enhance their effect against free-living bacteria as determined by the chequerboard method. The time-kill assay proved synergy between AgNPs and streptomycin only. The ability of AgNPs to induce the major chaperone protein DnaK may influence bacterial resistance to antimicrobials.},
  author       = {Markowska, Katarzyna and Grudniak, Anna M. and Krawczyk, Krzysztof and Wróbel, Izabela and Wolska, Krystyna I.},
  issn         = {0022-2615},
  language     = {eng},
  month        = {03},
  number       = {6},
  pages        = {849--854},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Medical Microbiology},
  title        = {Modulation of antibiotic resistance and induction of a stress response in Pseudomonas aeruginosa by silver nanoparticles},
  url          = {http://dx.doi.org/10.1099/jmm.0.068833-0},
  volume       = {63},
  year         = {2014},
}