Studies of in vivo electropermeabilization by gamma camera measurements of (99m)Tc-DTPA
Engström, P E LU ; Persson, B R LU
and Salford, L G
LU
(1999)
In Biochimica et Biophysica Acta
1473(2-3).
p.8-321
- Abstract
A protocol was developed to study the drug uptake from in vivo electropermeabilization at different settings of parameters influencing the uptake efficiency. Radiolabelled diethylenetriaminepentaacetic acid (DTPA) was used to trace the distribution and internalization of a hydrophilic drug after in vivo electropermeabilization. Skeletal muscle tissue in rat was treated with permeabilizing electric pulses before or after intravenous administration of (99m)Tc-DTPA. The drug accumulation in the treated volume was subsequently evaluated with a scintillation camera. The dependence of uptake on field strength and duration of the applied electric pulses was investigated for exponentially decaying pulses and square wave pulses. Further, the... (More)
A protocol was developed to study the drug uptake from in vivo electropermeabilization at different settings of parameters influencing the uptake efficiency. Radiolabelled diethylenetriaminepentaacetic acid (DTPA) was used to trace the distribution and internalization of a hydrophilic drug after in vivo electropermeabilization. Skeletal muscle tissue in rat was treated with permeabilizing electric pulses before or after intravenous administration of (99m)Tc-DTPA. The drug accumulation in the treated volume was subsequently evaluated with a scintillation camera. The dependence of uptake on field strength and duration of the applied electric pulses was investigated for exponentially decaying pulses and square wave pulses. Further, the uptake dependence on time interval between injection and pulsation was studied as well as the uptake dependence on the number of pulses applied in a single electropermeabilization treatment. Dynamic gamma camera studies were performed to quantify the time scale of the drug uptake in electropermeabilized tissue.
(Less)
- author
- Engström, P E
LU
; Persson, B R
LU
and Salford, L G
LU
- organization
- publishing date
- 1999-12-27
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Electroporation, Female, Gamma Cameras, Injections, Intravenous, Male, Muscle, Skeletal, Pentetic Acid, Rats, Rats, Inbred F344, Technetium Tc 99m Pentetate, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
- in
- Biochimica et Biophysica Acta
- volume
- 1473
- issue
- 2-3
- pages
- 8 - 321
- publisher
- Elsevier
- external identifiers
-
- pmid:10594369
- scopus:0032755834
- ISSN
- 0006-3002
- DOI
- 10.1016/S0304-4165(99)00199-3
- language
- English
- LU publication?
- yes
- id
- 88aa0489-282f-4cd8-925b-a79e1143bbcb
- date added to LUP
- 2017-04-05 14:22:52
- date last changed
- 2024-01-13 18:18:01
@article{88aa0489-282f-4cd8-925b-a79e1143bbcb,
abstract = {{<p>A protocol was developed to study the drug uptake from in vivo electropermeabilization at different settings of parameters influencing the uptake efficiency. Radiolabelled diethylenetriaminepentaacetic acid (DTPA) was used to trace the distribution and internalization of a hydrophilic drug after in vivo electropermeabilization. Skeletal muscle tissue in rat was treated with permeabilizing electric pulses before or after intravenous administration of (99m)Tc-DTPA. The drug accumulation in the treated volume was subsequently evaluated with a scintillation camera. The dependence of uptake on field strength and duration of the applied electric pulses was investigated for exponentially decaying pulses and square wave pulses. Further, the uptake dependence on time interval between injection and pulsation was studied as well as the uptake dependence on the number of pulses applied in a single electropermeabilization treatment. Dynamic gamma camera studies were performed to quantify the time scale of the drug uptake in electropermeabilized tissue.</p>}},
author = {{Engström, P E and Persson, B R and Salford, L G}},
issn = {{0006-3002}},
keywords = {{Animals; Electroporation; Female; Gamma Cameras; Injections, Intravenous; Male; Muscle, Skeletal; Pentetic Acid; Rats; Rats, Inbred F344; Technetium Tc 99m Pentetate; Time Factors; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{12}},
number = {{2-3}},
pages = {{8--321}},
publisher = {{Elsevier}},
series = {{Biochimica et Biophysica Acta}},
title = {{Studies of in vivo electropermeabilization by gamma camera measurements of (99m)Tc-DTPA}},
url = {{http://dx.doi.org/10.1016/S0304-4165(99)00199-3}},
doi = {{10.1016/S0304-4165(99)00199-3}},
volume = {{1473}},
year = {{1999}},
}