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Studies of in vivo electropermeabilization by gamma camera measurements of (99m)Tc-DTPA

Engström, P E LU ; Persson, B R LU and Salford, L G LU (1999) In Biochimica et biophysica acta 1473(2-3). p.8-321
Abstract

A protocol was developed to study the drug uptake from in vivo electropermeabilization at different settings of parameters influencing the uptake efficiency. Radiolabelled diethylenetriaminepentaacetic acid (DTPA) was used to trace the distribution and internalization of a hydrophilic drug after in vivo electropermeabilization. Skeletal muscle tissue in rat was treated with permeabilizing electric pulses before or after intravenous administration of (99m)Tc-DTPA. The drug accumulation in the treated volume was subsequently evaluated with a scintillation camera. The dependence of uptake on field strength and duration of the applied electric pulses was investigated for exponentially decaying pulses and square wave pulses. Further, the... (More)

A protocol was developed to study the drug uptake from in vivo electropermeabilization at different settings of parameters influencing the uptake efficiency. Radiolabelled diethylenetriaminepentaacetic acid (DTPA) was used to trace the distribution and internalization of a hydrophilic drug after in vivo electropermeabilization. Skeletal muscle tissue in rat was treated with permeabilizing electric pulses before or after intravenous administration of (99m)Tc-DTPA. The drug accumulation in the treated volume was subsequently evaluated with a scintillation camera. The dependence of uptake on field strength and duration of the applied electric pulses was investigated for exponentially decaying pulses and square wave pulses. Further, the uptake dependence on time interval between injection and pulsation was studied as well as the uptake dependence on the number of pulses applied in a single electropermeabilization treatment. Dynamic gamma camera studies were performed to quantify the time scale of the drug uptake in electropermeabilized tissue.

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publication status
published
keywords
Animals, Electroporation, Female, Gamma Cameras, Injections, Intravenous, Male, Muscle, Skeletal, Pentetic Acid, Rats, Rats, Inbred F344, Technetium Tc 99m Pentetate, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
in
Biochimica et biophysica acta
volume
1473
issue
2-3
pages
8 - 321
publisher
Elsevier
external identifiers
  • scopus:0032755834
ISSN
0006-3002
DOI
10.1016/S0304-4165(99)00199-3
language
English
LU publication?
yes
id
88aa0489-282f-4cd8-925b-a79e1143bbcb
date added to LUP
2017-04-05 14:22:52
date last changed
2017-08-20 05:09:57
@article{88aa0489-282f-4cd8-925b-a79e1143bbcb,
  abstract     = {<p>A protocol was developed to study the drug uptake from in vivo electropermeabilization at different settings of parameters influencing the uptake efficiency. Radiolabelled diethylenetriaminepentaacetic acid (DTPA) was used to trace the distribution and internalization of a hydrophilic drug after in vivo electropermeabilization. Skeletal muscle tissue in rat was treated with permeabilizing electric pulses before or after intravenous administration of (99m)Tc-DTPA. The drug accumulation in the treated volume was subsequently evaluated with a scintillation camera. The dependence of uptake on field strength and duration of the applied electric pulses was investigated for exponentially decaying pulses and square wave pulses. Further, the uptake dependence on time interval between injection and pulsation was studied as well as the uptake dependence on the number of pulses applied in a single electropermeabilization treatment. Dynamic gamma camera studies were performed to quantify the time scale of the drug uptake in electropermeabilized tissue.</p>},
  author       = {Engström, P E and Persson, B R and Salford, L G},
  issn         = {0006-3002},
  keyword      = {Animals,Electroporation,Female,Gamma Cameras,Injections, Intravenous,Male,Muscle, Skeletal,Pentetic Acid,Rats,Rats, Inbred F344,Technetium Tc 99m Pentetate,Time Factors,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {12},
  number       = {2-3},
  pages        = {8--321},
  publisher    = {Elsevier},
  series       = {Biochimica et biophysica acta},
  title        = {Studies of in vivo electropermeabilization by gamma camera measurements of (99m)Tc-DTPA},
  url          = {http://dx.doi.org/10.1016/S0304-4165(99)00199-3},
  volume       = {1473},
  year         = {1999},
}