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Synthesis and localization of pancreatic secretory proteins in pancreatic acinar-like metaplasia in the distal part of the oesophagus

Hakansson, HO; Mellblom, L; Johansson, J; Bjartell, Anders LU and Borgström, Anders LU (2003) In Scandinavian Journal of Gastroenterology 38(1). p.41560-41560
Abstract
Background: Pancreatic acinar-like metaplasia has previously been described in the gastric mucosa and in the distal part of the oesophagus. The resemblance to pancreatic acinar cells prompted us to study the possible occurrence of secretory pancreatic proteins in these cells. Methods: Seven specimens obtained from the distal oesophagus at gastroscopy where routine microscopy showed pancreatic acinar-like metaplasia were selected for this study. Sections were subjected to immunohistochemical detection of trypsinogen, pancreatic elastase, procarboxypeptidase B and pancreatic secretory trypsin inhibitor using specific antisera. An alkaline-phosphatase-conjugated oligodeoxynucleotide probe, complementary to the transcript for trypsinogen 2... (More)
Background: Pancreatic acinar-like metaplasia has previously been described in the gastric mucosa and in the distal part of the oesophagus. The resemblance to pancreatic acinar cells prompted us to study the possible occurrence of secretory pancreatic proteins in these cells. Methods: Seven specimens obtained from the distal oesophagus at gastroscopy where routine microscopy showed pancreatic acinar-like metaplasia were selected for this study. Sections were subjected to immunohistochemical detection of trypsinogen, pancreatic elastase, procarboxypeptidase B and pancreatic secretory trypsin inhibitor using specific antisera. An alkaline-phosphatase-conjugated oligodeoxynucleotide probe, complementary to the transcript for trypsinogen 2 (anionic) was used for in situ hybridization. Results: Cells with pancreatic acinar-like metaplasia were immunoreactive to all pancreatic secretory proteins studied. In situ hybridization showed the presence of trypsinogen 2 mRNA in pancreatic acinar-like metaplasia. The pancreatic proteins were not seen in other cells in the distal oesophagus. Conclusion: Pancreatic acinar-like metaplasia is common in the distal oesophagus and pancreatic secretory proteins, including trypsininogen 2, are produced in the oesophageal metaplastic acinar cells. The biological significance of this finding has yet not been thoroughly studied. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
intestinal metaplasia, trypsin, procarboxypeptidase B, inhibitor (PSTI), pancreatic secretory trypsin, pancreatic enzymes, pancreatic elastase, oesophagus, pancreatic acinar metaplasia (PAM)
in
Scandinavian Journal of Gastroenterology
volume
38
issue
1
pages
41560 - 41560
publisher
Taylor & Francis
external identifiers
  • wos:000180977000003
  • scopus:0037363691
ISSN
1502-7708
DOI
10.1080/00365520310000582
language
English
LU publication?
yes
id
b30a9a41-38f7-4cf9-a91e-35254f28243f (old id 891075)
date added to LUP
2008-01-12 15:26:36
date last changed
2017-01-01 07:22:52
@article{b30a9a41-38f7-4cf9-a91e-35254f28243f,
  abstract     = {Background: Pancreatic acinar-like metaplasia has previously been described in the gastric mucosa and in the distal part of the oesophagus. The resemblance to pancreatic acinar cells prompted us to study the possible occurrence of secretory pancreatic proteins in these cells. Methods: Seven specimens obtained from the distal oesophagus at gastroscopy where routine microscopy showed pancreatic acinar-like metaplasia were selected for this study. Sections were subjected to immunohistochemical detection of trypsinogen, pancreatic elastase, procarboxypeptidase B and pancreatic secretory trypsin inhibitor using specific antisera. An alkaline-phosphatase-conjugated oligodeoxynucleotide probe, complementary to the transcript for trypsinogen 2 (anionic) was used for in situ hybridization. Results: Cells with pancreatic acinar-like metaplasia were immunoreactive to all pancreatic secretory proteins studied. In situ hybridization showed the presence of trypsinogen 2 mRNA in pancreatic acinar-like metaplasia. The pancreatic proteins were not seen in other cells in the distal oesophagus. Conclusion: Pancreatic acinar-like metaplasia is common in the distal oesophagus and pancreatic secretory proteins, including trypsininogen 2, are produced in the oesophageal metaplastic acinar cells. The biological significance of this finding has yet not been thoroughly studied.},
  author       = {Hakansson, HO and Mellblom, L and Johansson, J and Bjartell, Anders and Borgström, Anders},
  issn         = {1502-7708},
  keyword      = {intestinal metaplasia,trypsin,procarboxypeptidase B,inhibitor (PSTI),pancreatic secretory trypsin,pancreatic enzymes,pancreatic elastase,oesophagus,pancreatic acinar metaplasia (PAM)},
  language     = {eng},
  number       = {1},
  pages        = {41560--41560},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Synthesis and localization of pancreatic secretory proteins in pancreatic acinar-like metaplasia in the distal part of the oesophagus},
  url          = {http://dx.doi.org/10.1080/00365520310000582},
  volume       = {38},
  year         = {2003},
}