Levels of neutrophil elastase and cathepsin B activities, and cystatins in human sputum: relationship to inflammation
(1990) In Scandinavian Journal of Clinical & Laboratory Investigation 50(5). p.509-516- Abstract
- Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B... (More)
- Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B activity in sputum is positively correlated with the degree of inflammation and neutrophil recruitment. Although this may be due in part to reduced amounts of cathepsin B inhibitors, particularly cystatin C, theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105552
- author
- Buttle, D J ; Burnett, D and Abrahamson, Magnus LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- agr2-macroglobulin, bronchiectasis, myeloperoxidase, neutrophil degranulation
- in
- Scandinavian Journal of Clinical & Laboratory Investigation
- volume
- 50
- issue
- 5
- pages
- 509 - 516
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:2237263
- scopus:0025105872
- ISSN
- 1502-7686
- DOI
- 10.3109/00365519009089165
- language
- English
- LU publication?
- yes
- id
- 8918d1f0-9c0f-4d53-b7ce-6ec0b2e32f35 (old id 1105552)
- date added to LUP
- 2016-04-01 16:14:02
- date last changed
- 2021-01-03 09:22:49
@article{8918d1f0-9c0f-4d53-b7ce-6ec0b2e32f35,
abstract = {{Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B activity in sputum is positively correlated with the degree of inflammation and neutrophil recruitment. Although this may be due in part to reduced amounts of cathepsin B inhibitors, particularly cystatin C, theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity.}},
author = {{Buttle, D J and Burnett, D and Abrahamson, Magnus}},
issn = {{1502-7686}},
keywords = {{agr2-macroglobulin; bronchiectasis; myeloperoxidase; neutrophil degranulation}},
language = {{eng}},
number = {{5}},
pages = {{509--516}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical & Laboratory Investigation}},
title = {{Levels of neutrophil elastase and cathepsin B activities, and cystatins in human sputum: relationship to inflammation}},
url = {{http://dx.doi.org/10.3109/00365519009089165}},
doi = {{10.3109/00365519009089165}},
volume = {{50}},
year = {{1990}},
}