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Relative concentrations of hK2/PSA mRNA in benign and malignant prostatic tissue

Lintula, S; Stenman, J; Bjartell, Anders LU ; Nordling, S and Stenman, UH (2005) In The Prostate 63(4). p.324-329
Abstract
BACKGROUND. Prostate-specific antigen (PSA/KLK3) and human kallikrein 2 (hK2/KLK2) belong to the human kallikrein gene family. These two highly homologous genes are specifically expressed in the prostate under androgen control. Expression of these is regulated by similar mechanisms but changes in their relative expression have been observed in prostate cancer. METHODS. We determined the relative levels of PSA and hK2 mRNA in benign and malignant prostate tissue using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. The mRNA of PSA and hK2 are reverse transcribed and amplified in one reaction with the same primers. RESULTS. The variation in the ratio of hK2/PSA mRNA was remarkably small, the difference between... (More)
BACKGROUND. Prostate-specific antigen (PSA/KLK3) and human kallikrein 2 (hK2/KLK2) belong to the human kallikrein gene family. These two highly homologous genes are specifically expressed in the prostate under androgen control. Expression of these is regulated by similar mechanisms but changes in their relative expression have been observed in prostate cancer. METHODS. We determined the relative levels of PSA and hK2 mRNA in benign and malignant prostate tissue using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. The mRNA of PSA and hK2 are reverse transcribed and amplified in one reaction with the same primers. RESULTS. The variation in the ratio of hK2/PSA mRNA was remarkably small, the difference between the highest and lowest values being three-fold. The ratio was significantly higher in WHO grade 2 compared to normal or benign prostatic hyperplasia tissue (P=0.032 and P=0.035, respectively) and in grade 3 compared to normal or benign prostatic hyperplasia tissue (P=0.006 in both). CONCLUSIONS. The new quantitative RT-PCR technique facilitates very accurate quantitation of the relative mRNA levels of homologous genes. Using this method we have shown that the ratio of hK2/PSA mRNA is higher in cancerous than in benign prostatic tissue. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
prostate tissue, BPH, human kallikreins, quantitative, RT-PCR
in
The Prostate
volume
63
issue
4
pages
324 - 329
publisher
John Wiley & Sons
external identifiers
  • wos:000229132800003
  • pmid:15599939
  • scopus:18644372502
ISSN
0270-4137
DOI
10.1002/pros.20194
language
English
LU publication?
yes
id
07eaf8f9-1072-4b6f-999c-4ad01d8bec88 (old id 895481)
date added to LUP
2008-01-16 13:47:17
date last changed
2017-10-01 03:49:23
@article{07eaf8f9-1072-4b6f-999c-4ad01d8bec88,
  abstract     = {BACKGROUND. Prostate-specific antigen (PSA/KLK3) and human kallikrein 2 (hK2/KLK2) belong to the human kallikrein gene family. These two highly homologous genes are specifically expressed in the prostate under androgen control. Expression of these is regulated by similar mechanisms but changes in their relative expression have been observed in prostate cancer. METHODS. We determined the relative levels of PSA and hK2 mRNA in benign and malignant prostate tissue using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. The mRNA of PSA and hK2 are reverse transcribed and amplified in one reaction with the same primers. RESULTS. The variation in the ratio of hK2/PSA mRNA was remarkably small, the difference between the highest and lowest values being three-fold. The ratio was significantly higher in WHO grade 2 compared to normal or benign prostatic hyperplasia tissue (P=0.032 and P=0.035, respectively) and in grade 3 compared to normal or benign prostatic hyperplasia tissue (P=0.006 in both). CONCLUSIONS. The new quantitative RT-PCR technique facilitates very accurate quantitation of the relative mRNA levels of homologous genes. Using this method we have shown that the ratio of hK2/PSA mRNA is higher in cancerous than in benign prostatic tissue.},
  author       = {Lintula, S and Stenman, J and Bjartell, Anders and Nordling, S and Stenman, UH},
  issn         = {0270-4137},
  keyword      = {prostate tissue,BPH,human kallikreins,quantitative,RT-PCR},
  language     = {eng},
  number       = {4},
  pages        = {324--329},
  publisher    = {John Wiley & Sons},
  series       = {The Prostate},
  title        = {Relative concentrations of hK2/PSA mRNA in benign and malignant prostatic tissue},
  url          = {http://dx.doi.org/10.1002/pros.20194},
  volume       = {63},
  year         = {2005},
}