Intranasal steroids decrease eosinophils but not mucin expression in nasal polyps
(2004) In European Respiratory Journal 24(4). p.594-600- Abstract
- Increased mucin expression is a feature of nasal polyposis. Corticosteroids reduce polyp size and symptoms, but their effect on mucin production remains unknown. In this study, the effects of intranasal corticosteroids on MUC5AC mucin expression, nasal resistance, eosinophil and neutrophil infiltration, epidermal growth factor receptor (EGFR), interleukin (IL)-8, and tumour necrosis factor (TNF)-alpha expression was assessed in nasal polyps. In nine subjects, one nasal polyp was removed surgically before treatment and another was removed after 8 weeks of intranasal fluticasone (400 mug.day(-1)). Tissues were processed for in situ hybridisation and immunohistochemical staining. Described effects of fluticasone on nasal polyps (reduction in... (More)
- Increased mucin expression is a feature of nasal polyposis. Corticosteroids reduce polyp size and symptoms, but their effect on mucin production remains unknown. In this study, the effects of intranasal corticosteroids on MUC5AC mucin expression, nasal resistance, eosinophil and neutrophil infiltration, epidermal growth factor receptor (EGFR), interleukin (IL)-8, and tumour necrosis factor (TNF)-alpha expression was assessed in nasal polyps. In nine subjects, one nasal polyp was removed surgically before treatment and another was removed after 8 weeks of intranasal fluticasone (400 mug.day(-1)). Tissues were processed for in situ hybridisation and immunohistochemical staining. Described effects of fluticasone on nasal polyps (reduction in nasal resistance and in eosinophil infiltration) were evaluated. Morphometric analysis was performed to assess the effect of fluticasone on epithelial-, MUC5AC-, EGFR- and IL-8-stained areas, TNF-alpha-stained cells, and neutrophil numbers. Treatment with fluticasone decreased nasal resistance and intra-epithelial eosinophils. The MUC5AC-stained area in the epithelium was unchanged by treatment; MUC5AC mRNA expression was unaffected by treatment. EGFR-stained area, intraepithelial neutrophil numbers, IL-8 and TNF-alpha expression were also unchanged by therapy. Intranasal fluticasone was effective in decreasing nasal airflow resistance and intra-epithelial eosinophils but had no effect on mucin or epidermal growth factor receptor expression or on neutrophil recruitment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/898138
- author
- Burgel, PR ; Cardell, Lars-Olaf LU ; Ueki, IF and Nadel, JA
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- steroid treatment, mucous hypersecretion, interleukin-8, airway epithelium, epidermal growth factor receptor, leukocyte recruitment
- in
- European Respiratory Journal
- volume
- 24
- issue
- 4
- pages
- 594 - 600
- publisher
- European Respiratory Society
- external identifiers
-
- wos:000224380300014
- pmid:15459138
- scopus:6444222548
- pmid:15459138
- ISSN
- 1399-3003
- DOI
- 10.1183/09031936.04.00014404
- language
- English
- LU publication?
- yes
- id
- a77dabc0-7c04-4b27-a287-fe60aa09cb37 (old id 898138)
- alternative location
- http://erj.ersjournals.com/cgi/content/abstract/24/4/594
- date added to LUP
- 2016-04-01 11:49:26
- date last changed
- 2022-02-03 05:35:16
@article{a77dabc0-7c04-4b27-a287-fe60aa09cb37,
abstract = {{Increased mucin expression is a feature of nasal polyposis. Corticosteroids reduce polyp size and symptoms, but their effect on mucin production remains unknown. In this study, the effects of intranasal corticosteroids on MUC5AC mucin expression, nasal resistance, eosinophil and neutrophil infiltration, epidermal growth factor receptor (EGFR), interleukin (IL)-8, and tumour necrosis factor (TNF)-alpha expression was assessed in nasal polyps. In nine subjects, one nasal polyp was removed surgically before treatment and another was removed after 8 weeks of intranasal fluticasone (400 mug.day(-1)). Tissues were processed for in situ hybridisation and immunohistochemical staining. Described effects of fluticasone on nasal polyps (reduction in nasal resistance and in eosinophil infiltration) were evaluated. Morphometric analysis was performed to assess the effect of fluticasone on epithelial-, MUC5AC-, EGFR- and IL-8-stained areas, TNF-alpha-stained cells, and neutrophil numbers. Treatment with fluticasone decreased nasal resistance and intra-epithelial eosinophils. The MUC5AC-stained area in the epithelium was unchanged by treatment; MUC5AC mRNA expression was unaffected by treatment. EGFR-stained area, intraepithelial neutrophil numbers, IL-8 and TNF-alpha expression were also unchanged by therapy. Intranasal fluticasone was effective in decreasing nasal airflow resistance and intra-epithelial eosinophils but had no effect on mucin or epidermal growth factor receptor expression or on neutrophil recruitment.}},
author = {{Burgel, PR and Cardell, Lars-Olaf and Ueki, IF and Nadel, JA}},
issn = {{1399-3003}},
keywords = {{steroid treatment; mucous hypersecretion; interleukin-8; airway epithelium; epidermal growth factor receptor; leukocyte recruitment}},
language = {{eng}},
number = {{4}},
pages = {{594--600}},
publisher = {{European Respiratory Society}},
series = {{European Respiratory Journal}},
title = {{Intranasal steroids decrease eosinophils but not mucin expression in nasal polyps}},
url = {{http://dx.doi.org/10.1183/09031936.04.00014404}},
doi = {{10.1183/09031936.04.00014404}},
volume = {{24}},
year = {{2004}},
}