Differentiation-specific, octamer-dependent costimulation of κ transcription
Liberg, David LU ; Sigvardsson, Mikael LU ; Bemark, Mats LU
and Leanderson, Tomas
LU
(1998)
In Journal of Immunology
160(8).
p.3899-3907
- Abstract
By mutational analysis of the octamer-TATA box intervening region in the mouse SP6 κ promoter, we have mapped two octamer-dependent, costimulatory regions, A and B. The A region was active in late B cells only, while the B region was active throughout B cell differentiation. The B region was TATA proximal and contained a heptamer and an E box of the E2A type that is common in Vκ promoters. Mutation of the heptamer element did not decrease transcriptional stimulation from this region, but mutations in, or immediately 5' of, the E box core sequence did. A protein binding to this region could be detected in nuclear extracts. The complex could only partially be competed with a μE5 binding site and could not be supershifted with Abs raised... (More)
By mutational analysis of the octamer-TATA box intervening region in the mouse SP6 κ promoter, we have mapped two octamer-dependent, costimulatory regions, A and B. The A region was active in late B cells only, while the B region was active throughout B cell differentiation. The B region was TATA proximal and contained a heptamer and an E box of the E2A type that is common in Vκ promoters. Mutation of the heptamer element did not decrease transcriptional stimulation from this region, but mutations in, or immediately 5' of, the E box core sequence did. A protein binding to this region could be detected in nuclear extracts. The complex could only partially be competed with a μE5 binding site and could not be supershifted with Abs raised to E2A gene products, indicating that it may represent a novel E-box binding complex. The A region was located proximal to the octamer and contained a CCCT element that is conserved both with regard to position and sequence in human VκII promoters. By mutational analysis, the transcriptional stimulatory activity was mapped to the CCCT element that also is part of an early B cell factor (EBF) binding site. In late B cells, a novel protein (FA), which did not bind to the EBF binding site in the mb1 promoter, interacted with the A region. This protein was found to be expressed at lower levels in early B cells as well as in HeLa cells. Thus, the octamer-flanking sequence contains positive control elements that may act independently but that differ in the stage of B cell differentiation at which they are active. One of these factors is an example of an ubiquitously expressed transcription factor that participate in differentiation-specific transcriptional activation.
(Less)
- author
- Liberg, David
LU
; Sigvardsson, Mikael
LU
; Bemark, Mats
LU
and Leanderson, Tomas
LU
- organization
- publishing date
- 1998-04-15
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 160
- issue
- 8
- pages
- 3899 - 3907
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:9558096
- scopus:0032522639
- ISSN
- 0022-1767
- DOI
- 10.4049/jimmunol.160.8.3899
- language
- English
- LU publication?
- yes
- id
- 898a0e76-7516-404e-8412-9d775e63fdee
- date added to LUP
- 2023-12-06 17:03:49
- date last changed
- 2024-01-04 15:27:11
@article{898a0e76-7516-404e-8412-9d775e63fdee,
abstract = {{<p>By mutational analysis of the octamer-TATA box intervening region in the mouse SP6 κ promoter, we have mapped two octamer-dependent, costimulatory regions, A and B. The A region was active in late B cells only, while the B region was active throughout B cell differentiation. The B region was TATA proximal and contained a heptamer and an E box of the E2A type that is common in Vκ promoters. Mutation of the heptamer element did not decrease transcriptional stimulation from this region, but mutations in, or immediately 5' of, the E box core sequence did. A protein binding to this region could be detected in nuclear extracts. The complex could only partially be competed with a μE5 binding site and could not be supershifted with Abs raised to E2A gene products, indicating that it may represent a novel E-box binding complex. The A region was located proximal to the octamer and contained a CCCT element that is conserved both with regard to position and sequence in human VκII promoters. By mutational analysis, the transcriptional stimulatory activity was mapped to the CCCT element that also is part of an early B cell factor (EBF) binding site. In late B cells, a novel protein (FA), which did not bind to the EBF binding site in the mb1 promoter, interacted with the A region. This protein was found to be expressed at lower levels in early B cells as well as in HeLa cells. Thus, the octamer-flanking sequence contains positive control elements that may act independently but that differ in the stage of B cell differentiation at which they are active. One of these factors is an example of an ubiquitously expressed transcription factor that participate in differentiation-specific transcriptional activation.</p>}},
author = {{Liberg, David and Sigvardsson, Mikael and Bemark, Mats and Leanderson, Tomas}},
issn = {{0022-1767}},
language = {{eng}},
month = {{04}},
number = {{8}},
pages = {{3899--3907}},
publisher = {{American Association of Immunologists}},
series = {{Journal of Immunology}},
title = {{Differentiation-specific, octamer-dependent costimulation of κ transcription}},
url = {{http://dx.doi.org/10.4049/jimmunol.160.8.3899}},
doi = {{10.4049/jimmunol.160.8.3899}},
volume = {{160}},
year = {{1998}},
}