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Oxytocin and oxytocin-receptor mRNA expression in the human gastrointestinal tract: a polymerase chain reaction study

Monstein, HJ; Grahn, N; Truedsson, M and Ohlsson, Bodil LU (2004) In Regulatory Peptides 119(1-2). p.39-44
Abstract
Background/aim: Oxytocin (OT) has a wide range of effects throughout the body. However, the role of OT on the gastrointestinal (GI) tract has to be settled. So far, the few studies performed reveal no conclusive results. The aim of this study was to examine the expression of Of and OT-receptor mRNA in the human GI tract. Material and methods: Full-thickness biopsies from all segments of the GI tract and the gallbladder were collected during operations at the Department Of Surgery, Malmo University Hospital. Biopsies were taken and put immediately into fluid nitrogen and stored at -70 degreesC until total RNA was extracted after mechanical tissue homogenization. Subsequently, poly A(+) mRNA was isolated from the total RNA extract using an... (More)
Background/aim: Oxytocin (OT) has a wide range of effects throughout the body. However, the role of OT on the gastrointestinal (GI) tract has to be settled. So far, the few studies performed reveal no conclusive results. The aim of this study was to examine the expression of Of and OT-receptor mRNA in the human GI tract. Material and methods: Full-thickness biopsies from all segments of the GI tract and the gallbladder were collected during operations at the Department Of Surgery, Malmo University Hospital. Biopsies were taken and put immediately into fluid nitrogen and stored at -70 degreesC until total RNA was extracted after mechanical tissue homogenization. Subsequently, poly A(+) mRNA was isolated from the total RNA extract using an automated nucleic acid extractor and converted into single-stranded cDNA. PCR amplifications were carried out using gene-specific OT and OT-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene specific OT and OT-receptor hybridization probes. Results: Expression of OT and OT-receptor mRNA was detected in nearly all segments of the GI tract analyzed. In most of the biopsy specimens analyzed, co-expression of both OT and OT-receptor mRNA appeared to take place. Conclusion: The present study demonstrates that OT and OT-receptor mRNAs are expressed throughout the GI tract. A possible physiological and/or pathophysiological role of OT and OT-receptor expression in the human GI tract and the cellular location of its expression remain to be shown. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oxytocin oxytocin receptor, inRNA expression, improved mRNA isolation, RT-PCR, gastrointestinal tract
in
Regulatory Peptides
volume
119
issue
1-2
pages
39 - 44
publisher
Elsevier
external identifiers
  • pmid:15093695
  • wos:000221090700006
  • scopus:1842684995
ISSN
1873-1686
DOI
10.1016/j.regpep.2003.12.017
language
English
LU publication?
yes
id
20759c12-031c-4719-b025-afde275c2b37 (old id 899043)
date added to LUP
2008-01-15 15:19:16
date last changed
2017-11-19 03:39:22
@article{20759c12-031c-4719-b025-afde275c2b37,
  abstract     = {Background/aim: Oxytocin (OT) has a wide range of effects throughout the body. However, the role of OT on the gastrointestinal (GI) tract has to be settled. So far, the few studies performed reveal no conclusive results. The aim of this study was to examine the expression of Of and OT-receptor mRNA in the human GI tract. Material and methods: Full-thickness biopsies from all segments of the GI tract and the gallbladder were collected during operations at the Department Of Surgery, Malmo University Hospital. Biopsies were taken and put immediately into fluid nitrogen and stored at -70 degreesC until total RNA was extracted after mechanical tissue homogenization. Subsequently, poly A(+) mRNA was isolated from the total RNA extract using an automated nucleic acid extractor and converted into single-stranded cDNA. PCR amplifications were carried out using gene-specific OT and OT-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene specific OT and OT-receptor hybridization probes. Results: Expression of OT and OT-receptor mRNA was detected in nearly all segments of the GI tract analyzed. In most of the biopsy specimens analyzed, co-expression of both OT and OT-receptor mRNA appeared to take place. Conclusion: The present study demonstrates that OT and OT-receptor mRNAs are expressed throughout the GI tract. A possible physiological and/or pathophysiological role of OT and OT-receptor expression in the human GI tract and the cellular location of its expression remain to be shown. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Monstein, HJ and Grahn, N and Truedsson, M and Ohlsson, Bodil},
  issn         = {1873-1686},
  keyword      = {oxytocin oxytocin receptor,inRNA expression,improved mRNA isolation,RT-PCR,gastrointestinal tract},
  language     = {eng},
  number       = {1-2},
  pages        = {39--44},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Oxytocin and oxytocin-receptor mRNA expression in the human gastrointestinal tract: a polymerase chain reaction study},
  url          = {http://dx.doi.org/10.1016/j.regpep.2003.12.017},
  volume       = {119},
  year         = {2004},
}