Lund University Publications

@article{8a87bf00-2ba7-4dab-8d5b-a768e8290ed5,
  abstract     = {{The Hessian fly, <i>Mayetiola destructor</i> Say (Diptera, <br>
Cecidomyiidae), is a pest of wheat and belongs to a group of <br>
gall-inducing herbivores. This species has a unique life history and <br>
several ecological features that differentiate it from other Diptera <br>
such as <i>Drosophila melanogaster</i> and blood-feeding mosquitoes. <br>
These features include a short, non-feeding adult life stage (1–2 days) <br>
and the use of a long-range sex pheromone produced and released by adult<br>
 females. Sex pheromones are detected by members of the odorant receptor<br>
 (OR) family within the Lepidoptera, but no receptors for similar <br>
long-range sex pheromones have been characterized from the Diptera. <br>
Previously, 122 OR genes have been annotated from the Hessian fly <br>
genome, with many of them showing sex-biased expression in the antennae.<br>
 Here we have expressed, in HEK293 cells, five MdesORs that display <br>
male-biased expression in antennae, and we have identified MdesOR115 as a<br>
 Hessian fly sex pheromone receptor. MdesOR115 responds primarily to the<br>
 sex pheromone component (2<i>S</i>,8<i>E</i>,10<i>E</i>)-8,10-tridecadien-2-yl acetate, and secondarily to the corresponding <i>Z</i>,<i>E</i>-isomer.<br>
 Certain sensory neuron membrane proteins (i.e., SNMP1) are important <br>
for responses of pheromone receptors in flies and moths. The Hessian fly<br>
 genome is unusual in that it encodes six SNMP1 paralogs, of which five <br>
are expressed in antennae. We co-expressed each of the five antennal <br>
SNMP1 paralogs together with each of the five candidate sex pheromone <br>
receptors from the Hessian fly and found that they do not influence the <br>
response of MdesOR115, nor do they confer responsiveness in any of the <br>
non-responsive ORs to any of the sex pheromone components identified to <br>
date in the Hessian fly. Using Western blots, we detected protein <br>
expression of MdesOrco, all MdesSNMPs, and all MdesORs except for <br>
MdesOR113, potentially explaining the lack of response from this OR. In <br>
conclusion, we report the first functional characterization of an OR <br>
from the Cecidomyiidae, extending the role of ORs as long-range sex <br>
pheromone detectors from the Lepidoptera into the Diptera.<br/><br/>}},
  author       = {{Andersson, Martin N and Corcoran, Jacob and Zhang, Dandan and Hillbur, Ylva and Newcomb, Richard D. and Löfstedt, Christer}},
  issn         = {{1662-5102}},
  keywords     = {{deorphanization; functional characterization; heterologous expression; HEK293 cells; odorant receptor; pheromone receptor; sensory neuron membrane protein}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{SEP2016}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Cellular Neuroscience}},
  title        = {{A sex pheromone receptor in the Hessian fly <i>Mayetiola destructor</i> (Diptera, Cecidomyiidae)}},
  url          = {{https://lup.lub.lu.se/search/files/12782900/A174.pdf}},
  doi          = {{10.3389/fncel.2016.00212}},
  volume       = {{10}},
  year         = {{2016}},
}