Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society.
(2011) In Clinical and Experimental Immunology 163. p.33-49- Abstract
- Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays are being developed for these purposes, it is important to optimize and standardize methods for processing human blood samples for these assays. To this end, we review data relevant to critical parameters in peripheral blood mononuclear cell (PBMC) isolation, (cryo)preservation, distribution and usage for detecting antigen-specific T cell responses. Based on these data, we propose recommendations on processing blood samples... (More)
- Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays are being developed for these purposes, it is important to optimize and standardize methods for processing human blood samples for these assays. To this end, we review data relevant to critical parameters in peripheral blood mononuclear cell (PBMC) isolation, (cryo)preservation, distribution and usage for detecting antigen-specific T cell responses. Based on these data, we propose recommendations on processing blood samples for T cell assays and identify gaps in knowledge that need to be addressed. These recommendations may be relevant not only for the analysis of T cell responses in autoimmune disease, but also in cancer and infectious disease, particularly in the context of clinical trials. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1711254
- author
- Mallone, R ; Mannering, S I ; Brooks-Worrell, B M ; Durinovic-Belló, I ; Cilio, Corrado LU ; Wong, F S and Schloot, N C
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical and Experimental Immunology
- volume
- 163
- pages
- 33 - 49
- publisher
- British Society for Immunology
- external identifiers
-
- wos:000284851800005
- pmid:20939860
- scopus:78649740257
- ISSN
- 0009-9104
- DOI
- 10.1111/j.1365-2249.2010.04272.x
- language
- English
- LU publication?
- yes
- id
- 8aae796c-ff1d-405d-87e5-81afa0a421b0 (old id 1711254)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20939860?dopt=Abstract
- date added to LUP
- 2016-04-04 08:55:15
- date last changed
- 2022-04-15 21:12:52
@article{8aae796c-ff1d-405d-87e5-81afa0a421b0,
abstract = {{Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays are being developed for these purposes, it is important to optimize and standardize methods for processing human blood samples for these assays. To this end, we review data relevant to critical parameters in peripheral blood mononuclear cell (PBMC) isolation, (cryo)preservation, distribution and usage for detecting antigen-specific T cell responses. Based on these data, we propose recommendations on processing blood samples for T cell assays and identify gaps in knowledge that need to be addressed. These recommendations may be relevant not only for the analysis of T cell responses in autoimmune disease, but also in cancer and infectious disease, particularly in the context of clinical trials.}},
author = {{Mallone, R and Mannering, S I and Brooks-Worrell, B M and Durinovic-Belló, I and Cilio, Corrado and Wong, F S and Schloot, N C}},
issn = {{0009-9104}},
language = {{eng}},
pages = {{33--49}},
publisher = {{British Society for Immunology}},
series = {{Clinical and Experimental Immunology}},
title = {{Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society.}},
url = {{http://dx.doi.org/10.1111/j.1365-2249.2010.04272.x}},
doi = {{10.1111/j.1365-2249.2010.04272.x}},
volume = {{163}},
year = {{2011}},
}