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EFFECT OF KIDNEY STEM CELLS ON BIOCHEMICAL ANALAYSIS AND SMAD2/3 PHOSPHORYLATION IN RATS WITH DIABETIC NEPHROPATHY

Rafiee, Zeinab LU ; Mansouri, Esrafil and Orazizadeh, Mahmoud (2021) 6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021) p.297-297
Abstract
Introduction: Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.
Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks... (More)
Introduction: Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.
Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks after induction of early diabetic nephropathy was evaluated by using standard western blotting.
Result: The cells significantly reduced blood nitrogen (BUN), serum creatinine (Scr) and 24 urinary proteins. The phosphorylation of smad2 and smad3 significantly down-regulated. PAS staining showed in the presence of adult kidney stem cells histopathological changes were improved.
Conclusion: Adult kidney stem cells may be a candidate for treatment of early DN to improve the kidney function and regenerating kidney tissues in DN rats.
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author
; and
organization
publishing date
type
Contribution to conference
publication status
in press
subject
keywords
Stem cell therapy, smad signaling, Diabetic nephropathy, kidney stem cells
pages
297 - 297
conference name
6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021)
conference location
Maastricht, Netherlands
conference dates
2021-11-15 - 2021-11-19
language
English
LU publication?
yes
id
8bc9698a-c02f-40c8-b8c1-a27fbb4f04c7
date added to LUP
2021-09-25 11:09:02
date last changed
2021-10-04 09:08:54
@misc{8bc9698a-c02f-40c8-b8c1-a27fbb4f04c7,
  abstract     = {{Introduction:  Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.<br/> Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks after induction of early diabetic nephropathy was evaluated by using standard western blotting.<br/>Result: The cells significantly reduced blood nitrogen (BUN), serum creatinine (Scr) and 24 urinary proteins. The phosphorylation of smad2 and smad3 significantly down-regulated. PAS staining showed in the presence of adult kidney stem cells histopathological changes were improved.<br/>Conclusion:  Adult kidney stem cells may be a candidate for treatment of early DN to improve the kidney function and regenerating kidney tissues in DN rats.<br/>}},
  author       = {{Rafiee, Zeinab and Mansouri, Esrafil and Orazizadeh, Mahmoud}},
  keywords     = {{Stem cell therapy; smad signaling; Diabetic nephropathy; kidney stem cells}},
  language     = {{eng}},
  pages        = {{297--297}},
  title        = {{EFFECT OF KIDNEY STEM CELLS ON BIOCHEMICAL ANALAYSIS AND SMAD2/3 PHOSPHORYLATION IN RATS WITH DIABETIC NEPHROPATHY}},
  year         = {{2021}},
}