Yersinia enterocolitica-mediated degradation of neutrophil extracellular traps (NETs)
(2015) In FEMS Microbiology Letters 362(23).- Abstract
Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was... (More)
Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was reduced, suggesting that degradation of NETs has occurred. This was confirmed by NET degradation assays with phorbol-myristate-acetate-pre-stimulated neutrophils. In addition, we found that the Yersinia supernatants were able to degrade purified plasmid DNA. The absence of Ca(2+) and Mg(2+) ions, but not that of a protease inhibitor cocktail, completely abolished NET degradation. We therefore postulate that Y. enterocolitica produces Ca(2+)/Mg(2+)-dependent NET-degrading nucleases as shown for some Gram-positive pathogens.
(Less)
- author
- Möllerherm, Helene ; Neumann, Ariane LU ; Schilcher, Katrin ; Blodkamp, Stefanie ; Zeitouni, Nathalie E ; Dersch, Petra ; Lüthje, Petra ; Naim, Hassan Y. ; Zinkernagel, Annelies S and von Köckritz-Blickwede, Maren
- publishing date
- 2015-12
- type
- Contribution to journal
- publication status
- published
- keywords
- Amino Acid Sequence, Bacterial Proteins, Calcium, Endodeoxyribonucleases, Extracellular Traps, Humans, Immunity, Innate, Magnesium, Membrane Proteins, Neutrophils, Sequence Alignment, Serogroup, Yersinia enterocolitica, Journal Article, Research Support, Non-U.S. Gov't
- in
- FEMS Microbiology Letters
- volume
- 362
- issue
- 23
- article number
- fnv192
- publisher
- Oxford University Press
- external identifiers
-
- scopus:84982104146
- pmid:26459885
- ISSN
- 1574-6968
- DOI
- 10.1093/femsle/fnv192
- language
- English
- LU publication?
- no
- id
- 8c040eaa-9e73-4a8a-ae8e-f9565263d954
- date added to LUP
- 2017-09-19 12:23:39
- date last changed
- 2024-06-24 00:23:54
@article{8c040eaa-9e73-4a8a-ae8e-f9565263d954,
abstract = {{<p>Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was reduced, suggesting that degradation of NETs has occurred. This was confirmed by NET degradation assays with phorbol-myristate-acetate-pre-stimulated neutrophils. In addition, we found that the Yersinia supernatants were able to degrade purified plasmid DNA. The absence of Ca(2+) and Mg(2+) ions, but not that of a protease inhibitor cocktail, completely abolished NET degradation. We therefore postulate that Y. enterocolitica produces Ca(2+)/Mg(2+)-dependent NET-degrading nucleases as shown for some Gram-positive pathogens.</p>}},
author = {{Möllerherm, Helene and Neumann, Ariane and Schilcher, Katrin and Blodkamp, Stefanie and Zeitouni, Nathalie E and Dersch, Petra and Lüthje, Petra and Naim, Hassan Y. and Zinkernagel, Annelies S and von Köckritz-Blickwede, Maren}},
issn = {{1574-6968}},
keywords = {{Amino Acid Sequence; Bacterial Proteins; Calcium; Endodeoxyribonucleases; Extracellular Traps; Humans; Immunity, Innate; Magnesium; Membrane Proteins; Neutrophils; Sequence Alignment; Serogroup; Yersinia enterocolitica; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{23}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Letters}},
title = {{Yersinia enterocolitica-mediated degradation of neutrophil extracellular traps (NETs)}},
url = {{http://dx.doi.org/10.1093/femsle/fnv192}},
doi = {{10.1093/femsle/fnv192}},
volume = {{362}},
year = {{2015}},
}