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Yersinia enterocolitica-mediated degradation of neutrophil extracellular traps (NETs)

Möllerherm, Helene; Neumann, Ariane LU ; Schilcher, Katrin; Blodkamp, Stefanie; Zeitouni, Nathalie E; Dersch, Petra; Lüthje, Petra; Naim, Hassan Y.; Zinkernagel, Annelies S and von Köckritz-Blickwede, Maren (2015) In FEMS Microbiology Letters 362(23).
Abstract

Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was... (More)

Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was reduced, suggesting that degradation of NETs has occurred. This was confirmed by NET degradation assays with phorbol-myristate-acetate-pre-stimulated neutrophils. In addition, we found that the Yersinia supernatants were able to degrade purified plasmid DNA. The absence of Ca(2+) and Mg(2+) ions, but not that of a protease inhibitor cocktail, completely abolished NET degradation. We therefore postulate that Y. enterocolitica produces Ca(2+)/Mg(2+)-dependent NET-degrading nucleases as shown for some Gram-positive pathogens.

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published
keywords
Amino Acid Sequence, Bacterial Proteins, Calcium, Endodeoxyribonucleases, Extracellular Traps, Humans, Immunity, Innate, Magnesium, Membrane Proteins, Neutrophils, Sequence Alignment, Serogroup, Yersinia enterocolitica, Journal Article, Research Support, Non-U.S. Gov't
in
FEMS Microbiology Letters
volume
362
issue
23
publisher
Elsevier
external identifiers
  • scopus:84982104146
ISSN
1574-6968
DOI
10.1093/femsle/fnv192
language
English
LU publication?
no
id
8c040eaa-9e73-4a8a-ae8e-f9565263d954
date added to LUP
2017-09-19 12:23:39
date last changed
2017-11-14 09:53:33
@article{8c040eaa-9e73-4a8a-ae8e-f9565263d954,
  abstract     = {<p>Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common Yersinia enterocolitica serotypes O:3, O:8 and O:9 were able to induce NETs in human blood-derived neutrophils during the first hour of co-incubation. At later time points, the NET amount was reduced, suggesting that degradation of NETs has occurred. This was confirmed by NET degradation assays with phorbol-myristate-acetate-pre-stimulated neutrophils. In addition, we found that the Yersinia supernatants were able to degrade purified plasmid DNA. The absence of Ca(2+) and Mg(2+) ions, but not that of a protease inhibitor cocktail, completely abolished NET degradation. We therefore postulate that Y. enterocolitica produces Ca(2+)/Mg(2+)-dependent NET-degrading nucleases as shown for some Gram-positive pathogens.</p>},
  articleno    = {fnv192},
  author       = {Möllerherm, Helene and Neumann, Ariane and Schilcher, Katrin and Blodkamp, Stefanie and Zeitouni, Nathalie E and Dersch, Petra and Lüthje, Petra and Naim, Hassan Y. and Zinkernagel, Annelies S and von Köckritz-Blickwede, Maren},
  issn         = {1574-6968},
  keyword      = {Amino Acid Sequence,Bacterial Proteins,Calcium,Endodeoxyribonucleases,Extracellular Traps,Humans,Immunity, Innate,Magnesium,Membrane Proteins,Neutrophils,Sequence Alignment,Serogroup,Yersinia enterocolitica,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {23},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Yersinia enterocolitica-mediated degradation of neutrophil extracellular traps (NETs)},
  url          = {http://dx.doi.org/10.1093/femsle/fnv192},
  volume       = {362},
  year         = {2015},
}