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Short-term ammonium supply induces cellular defence to prevent oxidative stress in Arabidopsis leaves

Podgórska, Anna; Burian, Maria; Rychter, Anna M.; Rasmusson, Allan G. LU and Szal, Bozena (2017) In Physiologia Plantarum 160(1). p.65-83
Abstract

Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and... (More)

Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and mitochondrial alternative electron transport pathways, indicating rapid but temporally separated changes in chloroplastic, mitochondrial and cytosolic ROS metabolism. The fast induction of antioxidant defences significantly lowered intracellular H2O2 levels, and thus protected Arabidopsis leaves from oxidative stress. On the other hand elevated extracellular ROS production in response to ammonium supply may be involved in signalling. The response pattern displays an intricate plasticity of Arabidopsis redox metabolism to minimise stress in responses to nutrient changes.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physiologia Plantarum
volume
160
issue
1
pages
65 - 83
publisher
Wiley Online Library
external identifiers
  • scopus:85013113245
  • wos:000401237200006
ISSN
0031-9317
DOI
10.1111/ppl.12538
language
English
LU publication?
yes
id
8ca8ea77-e7ba-4416-88c5-90a07055caa5
date added to LUP
2017-03-01 13:34:54
date last changed
2018-09-23 04:45:52
@article{8ca8ea77-e7ba-4416-88c5-90a07055caa5,
  abstract     = {<p>Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and mitochondrial alternative electron transport pathways, indicating rapid but temporally separated changes in chloroplastic, mitochondrial and cytosolic ROS metabolism. The fast induction of antioxidant defences significantly lowered intracellular H<sub>2</sub>O<sub>2</sub> levels, and thus protected Arabidopsis leaves from oxidative stress. On the other hand elevated extracellular ROS production in response to ammonium supply may be involved in signalling. The response pattern displays an intricate plasticity of Arabidopsis redox metabolism to minimise stress in responses to nutrient changes.</p>},
  author       = {Podgórska, Anna and Burian, Maria and Rychter, Anna M. and Rasmusson, Allan G. and Szal, Bozena},
  issn         = {0031-9317},
  language     = {eng},
  number       = {1},
  pages        = {65--83},
  publisher    = {Wiley Online Library},
  series       = {Physiologia Plantarum},
  title        = {Short-term ammonium supply induces cellular defence to prevent oxidative stress in Arabidopsis leaves},
  url          = {http://dx.doi.org/10.1111/ppl.12538},
  volume       = {160},
  year         = {2017},
}