Short-term ammonium supply induces cellular defence to prevent oxidative stress in Arabidopsis leaves
(2017) In Physiologia Plantarum 160(1). p.65-83- Abstract
Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and... (More)
Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and mitochondrial alternative electron transport pathways, indicating rapid but temporally separated changes in chloroplastic, mitochondrial and cytosolic ROS metabolism. The fast induction of antioxidant defences significantly lowered intracellular H2O2 levels, and thus protected Arabidopsis leaves from oxidative stress. On the other hand elevated extracellular ROS production in response to ammonium supply may be involved in signalling. The response pattern displays an intricate plasticity of Arabidopsis redox metabolism to minimise stress in responses to nutrient changes.
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- author
- Podgórska, Anna ; Burian, Maria ; Rychter, Anna M. ; Rasmusson, Allan G. LU and Szal, Bozena
- organization
- publishing date
- 2017-05
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physiologia Plantarum
- volume
- 160
- issue
- 1
- pages
- 65 - 83
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85013113245
- pmid:28008622
- wos:000401237200006
- ISSN
- 0031-9317
- DOI
- 10.1111/ppl.12538
- language
- English
- LU publication?
- yes
- id
- 8ca8ea77-e7ba-4416-88c5-90a07055caa5
- date added to LUP
- 2017-03-01 13:34:54
- date last changed
- 2024-03-31 05:05:04
@article{8ca8ea77-e7ba-4416-88c5-90a07055caa5,
abstract = {{<p>Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. This did not induce growth retardation, showing that Arabidopsis can compensate for the changes in redox metabolism associated with the variations in nitrogen redox status. During the first 3h of ammonium treatment, we observed distinct transient shifts in reactive oxygen species (ROS), low-mass antioxidants, ROS-scavenging enzymes, and mitochondrial alternative electron transport pathways, indicating rapid but temporally separated changes in chloroplastic, mitochondrial and cytosolic ROS metabolism. The fast induction of antioxidant defences significantly lowered intracellular H<sub>2</sub>O<sub>2</sub> levels, and thus protected Arabidopsis leaves from oxidative stress. On the other hand elevated extracellular ROS production in response to ammonium supply may be involved in signalling. The response pattern displays an intricate plasticity of Arabidopsis redox metabolism to minimise stress in responses to nutrient changes.</p>}},
author = {{Podgórska, Anna and Burian, Maria and Rychter, Anna M. and Rasmusson, Allan G. and Szal, Bozena}},
issn = {{0031-9317}},
language = {{eng}},
number = {{1}},
pages = {{65--83}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Physiologia Plantarum}},
title = {{Short-term ammonium supply induces cellular defence to prevent oxidative stress in Arabidopsis leaves}},
url = {{http://dx.doi.org/10.1111/ppl.12538}},
doi = {{10.1111/ppl.12538}},
volume = {{160}},
year = {{2017}},
}