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Design and synthesis of an artificial pulmonary pleura for high throughput studies in acellular human lungs

Wagner, Darcy E. LU ; Fenn, Spencer L.; Bonenfant, Nicholas R.; Marks, Elliot R.; Borg, Zachary D.; Saunders, Patrick; Oldinski, Rachael A. and Weiss, Daniel J. (2014) In Cellular and Molecular Bioengineering 7(2). p.184-195
Abstract

Whole organ decellularization of complex organs, such as lungs, presents a unique opportunity for use of acellular scaffolds for ex vivo tissue engineering or for studying cell-extracellular matrix interactions ex vivo. A growing body of literature investigating decellularizing and recellularizing rodent lungs has provided important proof of concept models and rodent lungs are readily available for high throughput studies. In contrast, comparable progress in large animal and human lungs has been impeded owing to more limited availability and difficulties in handling larger tissue. While the use of smaller segments of acellular large animal or human lungs would maximize usage from a single lung, excision of small acellular segments... (More)

Whole organ decellularization of complex organs, such as lungs, presents a unique opportunity for use of acellular scaffolds for ex vivo tissue engineering or for studying cell-extracellular matrix interactions ex vivo. A growing body of literature investigating decellularizing and recellularizing rodent lungs has provided important proof of concept models and rodent lungs are readily available for high throughput studies. In contrast, comparable progress in large animal and human lungs has been impeded owing to more limited availability and difficulties in handling larger tissue. While the use of smaller segments of acellular large animal or human lungs would maximize usage from a single lung, excision of small acellular segments compromises the integrity of the pleural layer, leaving the terminal ends of blood vessels and airways exposed. We have developed a novel pleural coating using non-toxic ionically crosslinked alginate or photocrosslinked methacrylated alginate which can be applied to excised acellular lung segments, permits inflation of small segments, and significantly enhances retention of cells inoculated through cannulated airways or blood vessels. Further, photocrosslinking methacrylated alginate, using eosin Y and triethanolamine at 530 nm wavelength, results in a mechanically stable pleural coating that permits effective cyclic 3-dimensional stretch, i.e., mechanical ventilation, of individual segments.

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Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Alginate, Artificial pleura, Decellularization, Lung, Ventilation
in
Cellular and Molecular Bioengineering
volume
7
issue
2
pages
184 - 195
publisher
Springer New York
external identifiers
  • scopus:84903783407
ISSN
1865-5025
DOI
10.1007/s12195-014-0323-1
language
English
LU publication?
no
id
8d169c62-8b23-4fa5-b62e-e5d09681c706
date added to LUP
2017-08-15 15:10:15
date last changed
2017-09-10 05:22:56
@article{8d169c62-8b23-4fa5-b62e-e5d09681c706,
  abstract     = {<p>Whole organ decellularization of complex organs, such as lungs, presents a unique opportunity for use of acellular scaffolds for ex vivo tissue engineering or for studying cell-extracellular matrix interactions ex vivo. A growing body of literature investigating decellularizing and recellularizing rodent lungs has provided important proof of concept models and rodent lungs are readily available for high throughput studies. In contrast, comparable progress in large animal and human lungs has been impeded owing to more limited availability and difficulties in handling larger tissue. While the use of smaller segments of acellular large animal or human lungs would maximize usage from a single lung, excision of small acellular segments compromises the integrity of the pleural layer, leaving the terminal ends of blood vessels and airways exposed. We have developed a novel pleural coating using non-toxic ionically crosslinked alginate or photocrosslinked methacrylated alginate which can be applied to excised acellular lung segments, permits inflation of small segments, and significantly enhances retention of cells inoculated through cannulated airways or blood vessels. Further, photocrosslinking methacrylated alginate, using eosin Y and triethanolamine at 530 nm wavelength, results in a mechanically stable pleural coating that permits effective cyclic 3-dimensional stretch, i.e., mechanical ventilation, of individual segments.</p>},
  author       = {Wagner, Darcy E. and Fenn, Spencer L. and Bonenfant, Nicholas R. and Marks, Elliot R. and Borg, Zachary D. and Saunders, Patrick and Oldinski, Rachael A. and Weiss, Daniel J.},
  issn         = {1865-5025},
  keyword      = {Alginate,Artificial pleura,Decellularization,Lung,Ventilation},
  language     = {eng},
  number       = {2},
  pages        = {184--195},
  publisher    = {Springer New York},
  series       = {Cellular and Molecular Bioengineering},
  title        = {Design and synthesis of an artificial pulmonary pleura for high throughput studies in acellular human lungs},
  url          = {http://dx.doi.org/10.1007/s12195-014-0323-1},
  volume       = {7},
  year         = {2014},
}