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Melanoidins isolated from heated potato fiber (Potex) affect human colon cancer cells growth via modulation of cell cycle and proliferation regulatory proteins

Langner, Ewa ; Nunes, Fernando M. ; Pozarowski, Piotr ; Kandefer-Szerszen, Martyna ; Pierzynowski, Stefan LU and Rzeski, Wojciech (2013) In Food and Chemical Toxicology 57. p.246-255
Abstract
Melanoidins are brown, nitrogen containing, high molecular weight end products of Maillard reaction with poorly established activity towards tumor cells. The goal of present study was to verify whether both heated potato fiber Potex extract (180 degrees C for 2 h) and melanoidins isolated from the extract exerts growth-inhibiting activity in human colon cancer cells in vitro. The cells of LS180 colon cancer cell line were tested upon treatment with roasted potato fiber extract (AM4) as well as with high (HMW) and low (LMW) molecular weight fractions isolated from the extract, since both may be regarded as/or contain melanoidins. The tested compounds at concentration of 1000 mu g/ml reduced cell growth down to 45%, 69% and 54%,... (More)
Melanoidins are brown, nitrogen containing, high molecular weight end products of Maillard reaction with poorly established activity towards tumor cells. The goal of present study was to verify whether both heated potato fiber Potex extract (180 degrees C for 2 h) and melanoidins isolated from the extract exerts growth-inhibiting activity in human colon cancer cells in vitro. The cells of LS180 colon cancer cell line were tested upon treatment with roasted potato fiber extract (AM4) as well as with high (HMW) and low (LMW) molecular weight fractions isolated from the extract, since both may be regarded as/or contain melanoidins. The tested compounds at concentration of 1000 mu g/ml reduced cell growth down to 45%, 69% and 54%, respectively. Furthermore, deregulated ERK1/2 signaling was revealed upon treatment. Moreover, multiple alternations in cell cycle regulators activity were found (i.e. cyclinD1, cyclin-dependent kinase 4 and 6, p21, p27, p53, pRb) leading to cell cycle cessation in GO phase. Importantly, LMW compounds revealed markedly stronger potential to alter specific molecular targets comparing to HMW compounds. Summarizing, the results emphasize that both high and low molecular weight melanoidins contribute to antiproliferative activity of heated potato fiber in LS180 colon cancer cells in vitro. (C) 2013 Elsevier Ltd. All rights reserved. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antiproliferative activity, Colon cancer, Cell cycle inhibition, Melanoidins, Potato fiber
in
Food and Chemical Toxicology
volume
57
pages
246 - 255
publisher
Elsevier
external identifiers
  • wos:000320498300031
  • scopus:84877314113
  • pmid:23583803
ISSN
0278-6915
DOI
10.1016/j.fct.2013.03.042
language
English
LU publication?
yes
id
8d617c52-445c-47ae-8248-e9b6889c44b2 (old id 3979639)
date added to LUP
2016-04-01 14:27:41
date last changed
2022-01-28 00:46:20
@article{8d617c52-445c-47ae-8248-e9b6889c44b2,
  abstract     = {{Melanoidins are brown, nitrogen containing, high molecular weight end products of Maillard reaction with poorly established activity towards tumor cells. The goal of present study was to verify whether both heated potato fiber Potex extract (180 degrees C for 2 h) and melanoidins isolated from the extract exerts growth-inhibiting activity in human colon cancer cells in vitro. The cells of LS180 colon cancer cell line were tested upon treatment with roasted potato fiber extract (AM4) as well as with high (HMW) and low (LMW) molecular weight fractions isolated from the extract, since both may be regarded as/or contain melanoidins. The tested compounds at concentration of 1000 mu g/ml reduced cell growth down to 45%, 69% and 54%, respectively. Furthermore, deregulated ERK1/2 signaling was revealed upon treatment. Moreover, multiple alternations in cell cycle regulators activity were found (i.e. cyclinD1, cyclin-dependent kinase 4 and 6, p21, p27, p53, pRb) leading to cell cycle cessation in GO phase. Importantly, LMW compounds revealed markedly stronger potential to alter specific molecular targets comparing to HMW compounds. Summarizing, the results emphasize that both high and low molecular weight melanoidins contribute to antiproliferative activity of heated potato fiber in LS180 colon cancer cells in vitro. (C) 2013 Elsevier Ltd. All rights reserved.}},
  author       = {{Langner, Ewa and Nunes, Fernando M. and Pozarowski, Piotr and Kandefer-Szerszen, Martyna and Pierzynowski, Stefan and Rzeski, Wojciech}},
  issn         = {{0278-6915}},
  keywords     = {{Antiproliferative activity; Colon cancer; Cell cycle inhibition; Melanoidins; Potato fiber}},
  language     = {{eng}},
  pages        = {{246--255}},
  publisher    = {{Elsevier}},
  series       = {{Food and Chemical Toxicology}},
  title        = {{Melanoidins isolated from heated potato fiber (Potex) affect human colon cancer cells growth via modulation of cell cycle and proliferation regulatory proteins}},
  url          = {{http://dx.doi.org/10.1016/j.fct.2013.03.042}},
  doi          = {{10.1016/j.fct.2013.03.042}},
  volume       = {{57}},
  year         = {{2013}},
}