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Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis

Urbansky, Anke LU ; Ohlsson, Pelle LU orcid ; Lenshof, Andreas LU orcid ; Garofalo, Fabio LU ; Scheding, Stefan LU and Laurell, Thomas LU (2017) In Scientific Reports 7(1).
Abstract

Effective separation methods for fractionating blood components are needed for numerous diagnostic and research applications. This paper presents the use of acoustophoresis, an ultrasound based microfluidic separation technology, for label-free, gentle and continuous separation of mononuclear cells (MNCs) from diluted whole blood. Red blood cells (RBCs) and MNCs behave similar in an acoustic standing wave field, compromising acoustic separation of MNC from RBC in standard buffer systems. However, by optimizing the buffer conditions and thereby changing the acoustophoretic mobility of the cells, we were able to enrich MNCs relative to RBCs by a factor of 2,800 with MNC recoveries up to 88%. The acoustophoretic microchip can perform cell... (More)

Effective separation methods for fractionating blood components are needed for numerous diagnostic and research applications. This paper presents the use of acoustophoresis, an ultrasound based microfluidic separation technology, for label-free, gentle and continuous separation of mononuclear cells (MNCs) from diluted whole blood. Red blood cells (RBCs) and MNCs behave similar in an acoustic standing wave field, compromising acoustic separation of MNC from RBC in standard buffer systems. However, by optimizing the buffer conditions and thereby changing the acoustophoretic mobility of the cells, we were able to enrich MNCs relative to RBCs by a factor of 2,800 with MNC recoveries up to 88%. The acoustophoretic microchip can perform cell separation at a processing rate of more than 1 × 105 cells/s, corresponding to 5 μl/min undiluted whole blood equivalent. Thus, acoustophoresis can be easily integrated with further down-stream applications such as flow cytometry, making it a superior alternative to existing MNC isolation techniques.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
7
issue
1
article number
17161
publisher
Nature Publishing Group
external identifiers
  • pmid:29215046
  • wos:000417354700007
  • scopus:85038108831
ISSN
2045-2322
DOI
10.1038/s41598-017-17200-9
language
English
LU publication?
yes
id
8f42a891-b6dc-49b3-8673-fc3a366bb8aa
date added to LUP
2018-01-03 14:48:07
date last changed
2025-03-05 10:28:59
@article{8f42a891-b6dc-49b3-8673-fc3a366bb8aa,
  abstract     = {{<p>Effective separation methods for fractionating blood components are needed for numerous diagnostic and research applications. This paper presents the use of acoustophoresis, an ultrasound based microfluidic separation technology, for label-free, gentle and continuous separation of mononuclear cells (MNCs) from diluted whole blood. Red blood cells (RBCs) and MNCs behave similar in an acoustic standing wave field, compromising acoustic separation of MNC from RBC in standard buffer systems. However, by optimizing the buffer conditions and thereby changing the acoustophoretic mobility of the cells, we were able to enrich MNCs relative to RBCs by a factor of 2,800 with MNC recoveries up to 88%. The acoustophoretic microchip can perform cell separation at a processing rate of more than 1 × 10<sup>5</sup> cells/s, corresponding to 5 μl/min undiluted whole blood equivalent. Thus, acoustophoresis can be easily integrated with further down-stream applications such as flow cytometry, making it a superior alternative to existing MNC isolation techniques.</p>}},
  author       = {{Urbansky, Anke and Ohlsson, Pelle and Lenshof, Andreas and Garofalo, Fabio and Scheding, Stefan and Laurell, Thomas}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis}},
  url          = {{http://dx.doi.org/10.1038/s41598-017-17200-9}},
  doi          = {{10.1038/s41598-017-17200-9}},
  volume       = {{7}},
  year         = {{2017}},
}