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Increased expression of vascular endothelial growth factor-a in seasonal allergic rhinitis

Benson, M; Carlsson, B; Carlsson, LMS; Wennergren, G and Cardell, Lars-Olaf LU (2002) In Cytokine 20(6). p.268-273
Abstract
Increased vascular dilatation and permeability characterize allergic rhinitis. In this study oligonucleotide microarrays (Affymetrix HuGe95A) were used to identify differentially expressed vasoactive genes in nasal biopsies from 23 patients with symptomatic seasonal allergic rhinitis (SAR) and 12 healthy controls. RNA was extracted from the biopsies and pooled in three patient and three control pools. Out of 12 626 analysed transcripts, 39 were higher and 81 lower in the patients. Of these transcripts two have vasoactive effects: Vascular Endothelial Growth Factor-A (VEGF-A) and the Beta-1-Adrenergic Receptor. Both were higher in patients than in controls. The mean +/- SEM expression levels in arbitrary units of VEGF-A were 130 123 in the... (More)
Increased vascular dilatation and permeability characterize allergic rhinitis. In this study oligonucleotide microarrays (Affymetrix HuGe95A) were used to identify differentially expressed vasoactive genes in nasal biopsies from 23 patients with symptomatic seasonal allergic rhinitis (SAR) and 12 healthy controls. RNA was extracted from the biopsies and pooled in three patient and three control pools. Out of 12 626 analysed transcripts, 39 were higher and 81 lower in the patients. Of these transcripts two have vasoactive effects: Vascular Endothelial Growth Factor-A (VEGF-A) and the Beta-1-Adrenergic Receptor. Both were higher in patients than in controls. The mean +/- SEM expression levels in arbitrary units of VEGF-A were 130 123 in the patients and 59 53 in the controls. The fold ratio in expression levels between patients/ controls was 2.2. The corresponding values for the beta-1-adrenergic receptor were 129 +/-123 in the patients and 40 +/- 31 in the controls. The fold ratio between patient/controls was 3.2. The role of VEGF-A was assessed by determining VEGF-A concentrations in nasal fluids from another 30 patients with SAR before and after allergen provocation. VEGF-A increased from 1243 +/- 30.2 to 163.2 +/- 37.8 pg/ml after challenge, P<0.05. In summary, oligonucleotide microarray analysis of nasal biopsies and protein analyses of nasal fluids indicate that VEGF-A may be an important mediator in SAR. (C) 2003 Elsevier Science Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
VEGF, allergic rhinitis, microarrays
in
Cytokine
volume
20
issue
6
pages
268 - 273
publisher
Academic Press
external identifiers
  • wos:000181699900003
  • pmid:12633568
  • scopus:0036910667
ISSN
1096-0023
DOI
10.1006/cyto.2002.1997
language
English
LU publication?
yes
id
20bb12ec-169f-4294-a0eb-9e99afd76961 (old id 900551)
date added to LUP
2008-01-17 10:41:58
date last changed
2017-12-10 04:37:07
@article{20bb12ec-169f-4294-a0eb-9e99afd76961,
  abstract     = {Increased vascular dilatation and permeability characterize allergic rhinitis. In this study oligonucleotide microarrays (Affymetrix HuGe95A) were used to identify differentially expressed vasoactive genes in nasal biopsies from 23 patients with symptomatic seasonal allergic rhinitis (SAR) and 12 healthy controls. RNA was extracted from the biopsies and pooled in three patient and three control pools. Out of 12 626 analysed transcripts, 39 were higher and 81 lower in the patients. Of these transcripts two have vasoactive effects: Vascular Endothelial Growth Factor-A (VEGF-A) and the Beta-1-Adrenergic Receptor. Both were higher in patients than in controls. The mean +/- SEM expression levels in arbitrary units of VEGF-A were 130 123 in the patients and 59 53 in the controls. The fold ratio in expression levels between patients/ controls was 2.2. The corresponding values for the beta-1-adrenergic receptor were 129 +/-123 in the patients and 40 +/- 31 in the controls. The fold ratio between patient/controls was 3.2. The role of VEGF-A was assessed by determining VEGF-A concentrations in nasal fluids from another 30 patients with SAR before and after allergen provocation. VEGF-A increased from 1243 +/- 30.2 to 163.2 +/- 37.8 pg/ml after challenge, P&lt;0.05. In summary, oligonucleotide microarray analysis of nasal biopsies and protein analyses of nasal fluids indicate that VEGF-A may be an important mediator in SAR. (C) 2003 Elsevier Science Ltd. All rights reserved.},
  author       = {Benson, M and Carlsson, B and Carlsson, LMS and Wennergren, G and Cardell, Lars-Olaf},
  issn         = {1096-0023},
  keyword      = {VEGF,allergic rhinitis,microarrays},
  language     = {eng},
  number       = {6},
  pages        = {268--273},
  publisher    = {Academic Press},
  series       = {Cytokine},
  title        = {Increased expression of vascular endothelial growth factor-a in seasonal allergic rhinitis},
  url          = {http://dx.doi.org/10.1006/cyto.2002.1997},
  volume       = {20},
  year         = {2002},
}