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Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis (Lep., Noctuidae)

Sadek, Medhat M. LU (2003) In Journal of Applied Entomology 127(7). p.396-404
Abstract
The effect of crude methanolic extracts of Adhatoda vasica leaves on the feeding and performance of Spodoptera littoralis larvae was investigated in the laboratory. Feeding on fresh leaves resulted in 100% mortality of larvae after 26 days of unsubstantial growth. The extract exhibited strong antifeedant and toxic activity against the larvae when applied either on leaf discs or incorporated into artificial diet. Under choice conditions the antifeedant index calculated over 72 h for neonate larvae increased significantly (from 71.5+/-3.2 to 92.1+/-4.2) as the concentration of extract in the treated diet increased from 200 to 1000 ppm. Consumption by the sixth instar larvae of leaf discs dipped in 0.01, 0.1 and 0.2% extract solutions was... (More)
The effect of crude methanolic extracts of Adhatoda vasica leaves on the feeding and performance of Spodoptera littoralis larvae was investigated in the laboratory. Feeding on fresh leaves resulted in 100% mortality of larvae after 26 days of unsubstantial growth. The extract exhibited strong antifeedant and toxic activity against the larvae when applied either on leaf discs or incorporated into artificial diet. Under choice conditions the antifeedant index calculated over 72 h for neonate larvae increased significantly (from 71.5+/-3.2 to 92.1+/-4.2) as the concentration of extract in the treated diet increased from 200 to 1000 ppm. Consumption by the sixth instar larvae of leaf discs dipped in 0.01, 0.1 and 0.2% extract solutions was significantly lower than consumption of control discs in both choice and no-choice tests. The latter two concentrations deterred feeding by 63.4 and 90.4%, respectively, under choice conditions, while only the 0.2% extract solution deterred feeding by 56.8% in the no-choice test. Toxicity of the extracts was manifested by a high mortality, reduced growth rates, and low weight gain by larvae fed on diets containing 200-2000 ppm of the extract. No larvae survived to pupation under the latter concentration. The time to pupation increased from 15.8+/-0.4 to 37.9+/-4.1 days as the extract concentration in diet increased from 0 to 1000 ppm. When fed to the fifth instar larvae, the crude extract significantly reduced consumption, growth, utilization of ingested and digested food, and approximate digestibility. The consumption-dependent growth efficiency of animals fed on extract-free diet was significantly higher than the growth efficiency of animals fed on extract-containing diets, suggesting both antifeedant and toxic activities of the extract. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adhatoda vasica, Acanthaceae, Spodoptera littoralis, antifeedant, nutritional indices, toxicity, extract, leaf
in
Journal of Applied Entomology
volume
127
issue
7
pages
396 - 404
publisher
Wiley-Blackwell
external identifiers
  • wos:000184519200004
  • scopus:0141458097
ISSN
0931-2048
DOI
language
English
LU publication?
yes
id
e8a093ca-fa00-42b5-840d-992ff86245b1 (old id 907387)
date added to LUP
2008-01-15 11:32:39
date last changed
2018-05-29 09:59:25
@article{e8a093ca-fa00-42b5-840d-992ff86245b1,
  abstract     = {The effect of crude methanolic extracts of Adhatoda vasica leaves on the feeding and performance of Spodoptera littoralis larvae was investigated in the laboratory. Feeding on fresh leaves resulted in 100% mortality of larvae after 26 days of unsubstantial growth. The extract exhibited strong antifeedant and toxic activity against the larvae when applied either on leaf discs or incorporated into artificial diet. Under choice conditions the antifeedant index calculated over 72 h for neonate larvae increased significantly (from 71.5+/-3.2 to 92.1+/-4.2) as the concentration of extract in the treated diet increased from 200 to 1000 ppm. Consumption by the sixth instar larvae of leaf discs dipped in 0.01, 0.1 and 0.2% extract solutions was significantly lower than consumption of control discs in both choice and no-choice tests. The latter two concentrations deterred feeding by 63.4 and 90.4%, respectively, under choice conditions, while only the 0.2% extract solution deterred feeding by 56.8% in the no-choice test. Toxicity of the extracts was manifested by a high mortality, reduced growth rates, and low weight gain by larvae fed on diets containing 200-2000 ppm of the extract. No larvae survived to pupation under the latter concentration. The time to pupation increased from 15.8+/-0.4 to 37.9+/-4.1 days as the extract concentration in diet increased from 0 to 1000 ppm. When fed to the fifth instar larvae, the crude extract significantly reduced consumption, growth, utilization of ingested and digested food, and approximate digestibility. The consumption-dependent growth efficiency of animals fed on extract-free diet was significantly higher than the growth efficiency of animals fed on extract-containing diets, suggesting both antifeedant and toxic activities of the extract.},
  author       = {Sadek, Medhat M.},
  issn         = {0931-2048},
  keyword      = {Adhatoda vasica,Acanthaceae,Spodoptera littoralis,antifeedant,nutritional indices,toxicity,extract,leaf},
  language     = {eng},
  number       = {7},
  pages        = {396--404},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Applied Entomology},
  title        = {Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis (Lep., Noctuidae)},
  url          = {http://dx.doi.org/},
  volume       = {127},
  year         = {2003},
}