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IL-1 beta induces murine airway 5-HT2A receptor hyperresponsiveness via a non-transcriptional MAPK-dependent mechanism

Zhang, Yaping LU ; Cardell, Lars-Olaf LU and Adner, Mikael LU (2007) In Respiratory Research 8.
Abstract
Background: Interleukin 1 beta ( IL- I beta) is found in bronchoalveolar lavage fluids from asthmatic patients and plays an important role in normal immunoregulatory processes but also in pathophysiological inflammatory responses. The present study was designed to investigate if IL- I beta could be involved in the development of airway hyperresponsiveness and if transcriptional mechanisms, epithelium contractile factors and mitogen-activated protein kinase ( MAPK) pathways are involved in IL- I beta effect. Methods: The effect of IL- I beta on 5-hydroxytryptamine ( 5- HT) induced bronchoconstriction was evaluated in an in-vitro model for assessment of long-term effects of inflammatory mediators on the airway smooth muscle. Murine tracheal... (More)
Background: Interleukin 1 beta ( IL- I beta) is found in bronchoalveolar lavage fluids from asthmatic patients and plays an important role in normal immunoregulatory processes but also in pathophysiological inflammatory responses. The present study was designed to investigate if IL- I beta could be involved in the development of airway hyperresponsiveness and if transcriptional mechanisms, epithelium contractile factors and mitogen-activated protein kinase ( MAPK) pathways are involved in IL- I beta effect. Methods: The effect of IL- I beta on 5-hydroxytryptamine ( 5- HT) induced bronchoconstriction was evaluated in an in-vitro model for assessment of long-term effects of inflammatory mediators on the airway smooth muscle. Murine tracheal segments were cultured up to 8 days in the absence or presence of IL- I beta with subsequent evaluation in a myograph system, along with mRNA quantification, focusing on the role of the epithelium, acetylcholine release, transcriptional mechanisms and MAPK activity. Results: During control conditions, 5- HT induced a relatively weak contraction. Presence of IL- I beta increased this response in a time-and concentration-dependent way. The increased concentration-effect curves could be shifted rightwards in a parallel manner by ketanserin, a selective 5- HT2A receptor antagonist, indicating that the responses are mediated by 5- HT2A receptors. The mRNA levels of 5- HT2A receptors were not changed as a consequence of the IL- I beta treatment and actinomycin D, a general transcriptional inhibitor, failed to affect the contractile response, suggesting a non-transcriptional mechanism behind this phenomenon. Neither the removal of the epithelium nor the addition of atropine affected the IL- I beta induced enhancement of 5- HT2A receptor-mediated contractile response. Application of inhibitors for c-Jun N-terminal kinase ( JNK), p38 and extracellular signal-regulated kinase 1 and 2 ( ERK1/2) showed that the signaling pathways for JNK and ERK1/2 dominated only in cultured segments ( control) whereas JNK and p38 dominated in segments treated with IL- I beta. Conclusion: IL- I beta induces murine airway hyperresponsiveness, via a non-transcriptional up-regulation of 5- HT2A receptor-mediated contractile response. The increase of 5- HT contraction is unrelated to epithelial and cholinergic factors, but is dependent on IL- I beta- induced changes of MAPK pathways. The fact that IL- I beta can alter airway responses to contractile agents such as 5- HT, via alteration of the intracellular MAPK signal transduction pathways, might provide a new concept for future treatment of asthma. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Respiratory Research
volume
8
publisher
BioMed Central
external identifiers
  • wos:000245720300001
  • scopus:34247842915
ISSN
1465-9921
DOI
10.1186/1465-9921-8-29
language
English
LU publication?
yes
id
47dbed8a-95f0-4062-bed1-12193107aef8 (old id 908049)
date added to LUP
2008-01-09 16:08:44
date last changed
2017-09-03 03:42:47
@article{47dbed8a-95f0-4062-bed1-12193107aef8,
  abstract     = {Background: Interleukin 1 beta ( IL- I beta) is found in bronchoalveolar lavage fluids from asthmatic patients and plays an important role in normal immunoregulatory processes but also in pathophysiological inflammatory responses. The present study was designed to investigate if IL- I beta could be involved in the development of airway hyperresponsiveness and if transcriptional mechanisms, epithelium contractile factors and mitogen-activated protein kinase ( MAPK) pathways are involved in IL- I beta effect. Methods: The effect of IL- I beta on 5-hydroxytryptamine ( 5- HT) induced bronchoconstriction was evaluated in an in-vitro model for assessment of long-term effects of inflammatory mediators on the airway smooth muscle. Murine tracheal segments were cultured up to 8 days in the absence or presence of IL- I beta with subsequent evaluation in a myograph system, along with mRNA quantification, focusing on the role of the epithelium, acetylcholine release, transcriptional mechanisms and MAPK activity. Results: During control conditions, 5- HT induced a relatively weak contraction. Presence of IL- I beta increased this response in a time-and concentration-dependent way. The increased concentration-effect curves could be shifted rightwards in a parallel manner by ketanserin, a selective 5- HT2A receptor antagonist, indicating that the responses are mediated by 5- HT2A receptors. The mRNA levels of 5- HT2A receptors were not changed as a consequence of the IL- I beta treatment and actinomycin D, a general transcriptional inhibitor, failed to affect the contractile response, suggesting a non-transcriptional mechanism behind this phenomenon. Neither the removal of the epithelium nor the addition of atropine affected the IL- I beta induced enhancement of 5- HT2A receptor-mediated contractile response. Application of inhibitors for c-Jun N-terminal kinase ( JNK), p38 and extracellular signal-regulated kinase 1 and 2 ( ERK1/2) showed that the signaling pathways for JNK and ERK1/2 dominated only in cultured segments ( control) whereas JNK and p38 dominated in segments treated with IL- I beta. Conclusion: IL- I beta induces murine airway hyperresponsiveness, via a non-transcriptional up-regulation of 5- HT2A receptor-mediated contractile response. The increase of 5- HT contraction is unrelated to epithelial and cholinergic factors, but is dependent on IL- I beta- induced changes of MAPK pathways. The fact that IL- I beta can alter airway responses to contractile agents such as 5- HT, via alteration of the intracellular MAPK signal transduction pathways, might provide a new concept for future treatment of asthma.},
  author       = {Zhang, Yaping and Cardell, Lars-Olaf and Adner, Mikael},
  issn         = {1465-9921},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {Respiratory Research},
  title        = {IL-1 beta induces murine airway 5-HT2A receptor hyperresponsiveness via a non-transcriptional MAPK-dependent mechanism},
  url          = {http://dx.doi.org/10.1186/1465-9921-8-29},
  volume       = {8},
  year         = {2007},
}