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Calmodulin and In Vitro Regenerating Frog Sciatic Nerves : Release and Extracellular Effects

Remgård, Pär; Ekström, Per LU ; Wiklund, Peter LU and Edström, Anders LU (1995) In European Journal of Neuroscience 7(6). p.1386-1392
Abstract

Although calmodulin (CaM) is commonly considered to be an intracellular protein, it has been suggested lately that it is released and exerts functions extracellularly. In the present investigation this was studied in in vitro regenerating adult frog (Rana temporaria) sciatic nerves. Using a multi‐compartment incubation chamber, the non‐neuronal cells in the outgrowth region of such nerves were radiolabelled with amino acid precursors. Based on immunological criteria, these cells were shown to release CaM. When the nerves were treated with CaM, both the outgrowth of sensory axons and the injury‐induced proliferation of non‐neuronal cells were partially inhibited. The inhibitory effects occurred even when the incubation medium contained... (More)

Although calmodulin (CaM) is commonly considered to be an intracellular protein, it has been suggested lately that it is released and exerts functions extracellularly. In the present investigation this was studied in in vitro regenerating adult frog (Rana temporaria) sciatic nerves. Using a multi‐compartment incubation chamber, the non‐neuronal cells in the outgrowth region of such nerves were radiolabelled with amino acid precursors. Based on immunological criteria, these cells were shown to release CaM. When the nerves were treated with CaM, both the outgrowth of sensory axons and the injury‐induced proliferation of non‐neuronal cells were partially inhibited. The inhibitory effects occurred even when the incubation medium contained as little as 30 pM CaM. Furthermore, treatment with anti‐CaM antibodies resulted in reduced outgrowth, which suggested that during normal conditions extracellular CaM is kept at an optimal concentration. Finally, conditioned medium was found to contain several CaM‐binding proteins. The present findings may reflect an earlier unknown function of extracellular CaM in controlling various growth mechanisms in integrated tissues. Copyright © 1995, Wiley Blackwell. All rights reserved

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
axon, outgrowth, proliferation, Rana temporaria, Schwann cells
in
European Journal of Neuroscience
volume
7
issue
6
pages
7 pages
publisher
Wiley-Blackwell
external identifiers
  • Scopus:0029056056
ISSN
0953-816X
DOI
10.1111/j.1460-9568.1995.tb01130.x
language
English
LU publication?
yes
id
909a12ec-7f66-4e67-b0a6-d94f15a4da9c
date added to LUP
2016-12-07 14:54:08
date last changed
2017-01-26 17:20:43
@article{909a12ec-7f66-4e67-b0a6-d94f15a4da9c,
  abstract     = {<p>Although calmodulin (CaM) is commonly considered to be an intracellular protein, it has been suggested lately that it is released and exerts functions extracellularly. In the present investigation this was studied in in vitro regenerating adult frog (Rana temporaria) sciatic nerves. Using a multi‐compartment incubation chamber, the non‐neuronal cells in the outgrowth region of such nerves were radiolabelled with amino acid precursors. Based on immunological criteria, these cells were shown to release CaM. When the nerves were treated with CaM, both the outgrowth of sensory axons and the injury‐induced proliferation of non‐neuronal cells were partially inhibited. The inhibitory effects occurred even when the incubation medium contained as little as 30 pM CaM. Furthermore, treatment with anti‐CaM antibodies resulted in reduced outgrowth, which suggested that during normal conditions extracellular CaM is kept at an optimal concentration. Finally, conditioned medium was found to contain several CaM‐binding proteins. The present findings may reflect an earlier unknown function of extracellular CaM in controlling various growth mechanisms in integrated tissues. Copyright © 1995, Wiley Blackwell. All rights reserved</p>},
  author       = {Remgård, Pär and Ekström, Per and Wiklund, Peter and Edström, Anders},
  issn         = {0953-816X},
  keyword      = {axon,outgrowth,proliferation,Rana temporaria,Schwann cells},
  language     = {eng},
  number       = {6},
  pages        = {1386--1392},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {Calmodulin and In Vitro Regenerating Frog Sciatic Nerves : Release and Extracellular Effects},
  url          = {http://dx.doi.org/10.1111/j.1460-9568.1995.tb01130.x},
  volume       = {7},
  year         = {1995},
}