Protein expression analysis: from "tip of the iceberg" to a global method.
James, Peter LU
(2001)
In Disease Markers
17(4).
p.235-246
- Abstract
- In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and... (More)
- In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and new mass spectrometers, this emerging technology will be a rapid and highly effective alternative to gel-based methods with few of the latter's shortcomings. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107106
- author
- James, Peter
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gel, Two-Dimensional : trends, Genome, Proteins : metabolism, Electrophoresis
- in
- Disease Markers
- volume
- 17
- issue
- 4
- pages
- 235 - 246
- publisher
- IOS Press
- external identifiers
-
- wos:000173434400005
- scopus:0035660059
- ISSN
- 0278-0240
- language
- English
- LU publication?
- yes
- id
- 91991fdd-9879-419d-a2e4-9dad1879321d (old id 107106)
- alternative location
- http://iospress.metapress.com/openurl.asp?genre=article&issn=0278-0240&volume=17&issue=4&spage=235
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11790891&dopt=Abstract
- date added to LUP
- 2016-04-01 17:00:49
- date last changed
- 2023-09-05 05:33:43
@article{91991fdd-9879-419d-a2e4-9dad1879321d,
abstract = {{In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and new mass spectrometers, this emerging technology will be a rapid and highly effective alternative to gel-based methods with few of the latter's shortcomings.}},
author = {{James, Peter}},
issn = {{0278-0240}},
keywords = {{Gel; Two-Dimensional : trends; Genome; Proteins : metabolism; Electrophoresis}},
language = {{eng}},
number = {{4}},
pages = {{235--246}},
publisher = {{IOS Press}},
series = {{Disease Markers}},
title = {{Protein expression analysis: from "tip of the iceberg" to a global method.}},
url = {{http://iospress.metapress.com/openurl.asp?genre=article&issn=0278-0240&volume=17&issue=4&spage=235}},
volume = {{17}},
year = {{2001}},
}