Transcatheter aortic valve implantation (TAVI) prostheses in vitro - biofilm formation and antibiotic effects
Sunnerhagen, Torgny LU
; Bjarnsholt, Thomas
; Qvortrup, Klaus
; Bundgaard, Henning
and Moser, Claus
(2024)
In Biofilm
8.
- Abstract
- Background
Transcatheter aortic valve implantation (TAVI) is a percutaneous catheter-based treatment of aortic stenosis as an alternative to open heart valve surgery. In cases of TAVI endocarditis, the treatment possibilities may be limited as surgical removal of the infected valve may be associated with a high risk in elderly, comorbid or frail patients. The propensity of bacteria to form a biofilm on foreign material is assumed to be of importance part of the disease process in TAVI endocarditis, but no studies on biofilm formation on TAVI valves have been conducted. We hypothesize that Staphylococcus aureus and Enterococcus faecalis biofilm formation on TAVI valves may have an impact on antibiotic tolerance and non-surgical cure... (More) - Background
Transcatheter aortic valve implantation (TAVI) is a percutaneous catheter-based treatment of aortic stenosis as an alternative to open heart valve surgery. In cases of TAVI endocarditis, the treatment possibilities may be limited as surgical removal of the infected valve may be associated with a high risk in elderly, comorbid or frail patients. The propensity of bacteria to form a biofilm on foreign material is assumed to be of importance part of the disease process in TAVI endocarditis, but no studies on biofilm formation on TAVI valves have been conducted. We hypothesize that Staphylococcus aureus and Enterococcus faecalis biofilm formation on TAVI valves may have an impact on antibiotic tolerance and non-surgical cure rates.
Methods
TAVI valves (pieces including part of the metal frame, approximately 1 cm wide) were exposed to either species in vitro in LB-Krebs Ringer medium at 37 °C, with the bacterial count being assessed by culturing of sonicated TAVI pieces and broth at 0, 4, 18 and 24 h after bacterial exposure. Scanning electron microscopy (SEM) was performed. Effects of ampicillin, gentamicin, moxifloxacin, rifampicin (for S. aureus), and ceftriaxone (for E. faecalis) at 5 times minimal inhibitory concentration were tested alone and in combination with ampicillin. Antibiotics were added to biofilm aged 0 or 24 h and the effects assessed.
Results
Exposure for 15 min established attachment to all of valve pieces. SEM findings were consistent with biofilm formation and suggested lower amounts of bacteria on the metal compared to the tissue part of the TAVI valves. The number of bacteria attached to the TAVI valves increased until 24 h of incubation from less than 10^1 to a level of approximately 10^9 CFU/g. The bacteria became more tolerant to antibiotics on the TAVI valves over time, with the bactericidal effect against 24-h old biofilm being significantly less effective than against 0-h old biofilm depending on antibiotic.
Conclusions
The results indicate that bacteria can adhere to metal and tissue parts of the TAVI valves within minutes after an exposure which is comparable to transient bacteremia in vivo, and that the bacteria rapidly gain biofilm properties, associated with significantly reduced antibiotic effect. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/919b2d53-3d2e-44cd-a8ef-90d5197ab6f9
- author
- Sunnerhagen, Torgny
LU
; Bjarnsholt, Thomas
; Qvortrup, Klaus
; Bundgaard, Henning
and Moser, Claus
- organization
- publishing date
- 2024-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biofilm
- volume
- 8
- article number
- 100236
- publisher
- Elsevier
- external identifiers
-
- scopus:85207859960
- pmid:39555138
- ISSN
- 2590-2075
- DOI
- 10.1016/j.bioflm.2024.100236
- project
- Matters of the heart- Biofilm on cardiovascular prostheses, their microbiological properties, and antibiotic tolerance
- language
- English
- LU publication?
- yes
- id
- 919b2d53-3d2e-44cd-a8ef-90d5197ab6f9
- alternative location
- https://linkinghub.elsevier.com/retrieve/pii/S2590207524000613
- date added to LUP
- 2024-11-01 19:38:57
- date last changed
- 2025-04-04 14:30:33
@article{919b2d53-3d2e-44cd-a8ef-90d5197ab6f9,
abstract = {{Background<br/>Transcatheter aortic valve implantation (TAVI) is a percutaneous catheter-based treatment of aortic stenosis as an alternative to open heart valve surgery. In cases of TAVI endocarditis, the treatment possibilities may be limited as surgical removal of the infected valve may be associated with a high risk in elderly, comorbid or frail patients. The propensity of bacteria to form a biofilm on foreign material is assumed to be of importance part of the disease process in TAVI endocarditis, but no studies on biofilm formation on TAVI valves have been conducted. We hypothesize that Staphylococcus aureus and Enterococcus faecalis biofilm formation on TAVI valves may have an impact on antibiotic tolerance and non-surgical cure rates.<br/>Methods<br/>TAVI valves (pieces including part of the metal frame, approximately 1 cm wide) were exposed to either species in vitro in LB-Krebs Ringer medium at 37 °C, with the bacterial count being assessed by culturing of sonicated TAVI pieces and broth at 0, 4, 18 and 24 h after bacterial exposure. Scanning electron microscopy (SEM) was performed. Effects of ampicillin, gentamicin, moxifloxacin, rifampicin (for S. aureus), and ceftriaxone (for E. faecalis) at 5 times minimal inhibitory concentration were tested alone and in combination with ampicillin. Antibiotics were added to biofilm aged 0 or 24 h and the effects assessed.<br/>Results<br/>Exposure for 15 min established attachment to all of valve pieces. SEM findings were consistent with biofilm formation and suggested lower amounts of bacteria on the metal compared to the tissue part of the TAVI valves. The number of bacteria attached to the TAVI valves increased until 24 h of incubation from less than 10^1 to a level of approximately 10^9 CFU/g. The bacteria became more tolerant to antibiotics on the TAVI valves over time, with the bactericidal effect against 24-h old biofilm being significantly less effective than against 0-h old biofilm depending on antibiotic.<br/>Conclusions<br/>The results indicate that bacteria can adhere to metal and tissue parts of the TAVI valves within minutes after an exposure which is comparable to transient bacteremia in vivo, and that the bacteria rapidly gain biofilm properties, associated with significantly reduced antibiotic effect.}},
author = {{Sunnerhagen, Torgny and Bjarnsholt, Thomas and Qvortrup, Klaus and Bundgaard, Henning and Moser, Claus}},
issn = {{2590-2075}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Biofilm}},
title = {{Transcatheter aortic valve implantation (TAVI) prostheses in vitro - biofilm formation and antibiotic effects}},
url = {{https://lup.lub.lu.se/search/files/198907045/1-s2.0-S2590207524000613-main.pdf}},
doi = {{10.1016/j.bioflm.2024.100236}},
volume = {{8}},
year = {{2024}},
}