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Impact of degradable nanowires on long-term brain tissue responses

Gällentoft, Lina LU ; Pettersson, Lina M E LU ; Danielsen, Nils LU ; Schouenborg, Jens LU ; Prinz, Christelle N. LU and Eriksson, Cecilia LU (2016) In Journal of Nanobiotechnology 14(1).
Abstract

Background: A promising approach to improve the performance of neural implants consists of adding nanomaterials, such as nanowires, to the surface of the implant. Nanostructured interfaces could improve the integration and communication stability, partly through the reduction of the cell-to-electrode distance. However, the safety issues of implanted nanowires in the brain need to be evaluated and understood before nanowires can be used on the surface of implants for long periods of time. To this end we here investigate whether implanted degradable nanowires offer any advantage over non-degradable nanowires in a long-term in vivo study (1 year) with respect to brain tissue responses. Results: The tissue response after injection of... (More)

Background: A promising approach to improve the performance of neural implants consists of adding nanomaterials, such as nanowires, to the surface of the implant. Nanostructured interfaces could improve the integration and communication stability, partly through the reduction of the cell-to-electrode distance. However, the safety issues of implanted nanowires in the brain need to be evaluated and understood before nanowires can be used on the surface of implants for long periods of time. To this end we here investigate whether implanted degradable nanowires offer any advantage over non-degradable nanowires in a long-term in vivo study (1 year) with respect to brain tissue responses. Results: The tissue response after injection of degradable silicon oxide (SiOx)-coated gallium phosphide nanowires and biostable hafnium oxide-coated GaP nanowires into the rat striatum was compared. One year after nanowire injection, no significant difference in microglial or astrocytic response, as measured by staining for ED1 and glial fibrillary acidic protein, respectively, or in neuronal density, as measured by staining for NeuN, was found between degradable and biostable nanowires. Of the cells investigated, only microglia cells had engulfed the nanowires. The SiOx-coated nanowire residues were primarily seen in aggregated hypertrophic ED1-positive cells, possibly microglial cells that have fused to create multinucleated giant cells. Occasionally, degradable nanowires with an apparently intact shape were found inside single, small ED1-positive cells. The biostable nanowires were found intact in microglia cells of both phenotypes described. Conclusion: The present study shows that the degradable nanowires remain at least partly in the brain over long time periods, i.e. 1 year; however, no obvious bio-safety issues for this degradable nanomaterial could be detected.

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Contribution to journal
publication status
published
subject
keywords
Biocompatibility, Biomaterial, Brain, Foreign body reaction, Immunohistochemistry, Nanomedicine, Nanowires, Neural interfaces, Tissue responses
in
Journal of Nanobiotechnology
volume
14
issue
1
publisher
BioMed Central
external identifiers
  • scopus:84988419350
  • wos:000381674500001
DOI
10.1186/s12951-016-0216-7
language
English
LU publication?
yes
id
91d11081-9df0-4637-be3b-03789b28d690
date added to LUP
2016-12-28 12:19:02
date last changed
2017-09-18 11:33:39
@article{91d11081-9df0-4637-be3b-03789b28d690,
  abstract     = {<p>Background: A promising approach to improve the performance of neural implants consists of adding nanomaterials, such as nanowires, to the surface of the implant. Nanostructured interfaces could improve the integration and communication stability, partly through the reduction of the cell-to-electrode distance. However, the safety issues of implanted nanowires in the brain need to be evaluated and understood before nanowires can be used on the surface of implants for long periods of time. To this end we here investigate whether implanted degradable nanowires offer any advantage over non-degradable nanowires in a long-term in vivo study (1 year) with respect to brain tissue responses. Results: The tissue response after injection of degradable silicon oxide (SiOx)-coated gallium phosphide nanowires and biostable hafnium oxide-coated GaP nanowires into the rat striatum was compared. One year after nanowire injection, no significant difference in microglial or astrocytic response, as measured by staining for ED1 and glial fibrillary acidic protein, respectively, or in neuronal density, as measured by staining for NeuN, was found between degradable and biostable nanowires. Of the cells investigated, only microglia cells had engulfed the nanowires. The SiOx-coated nanowire residues were primarily seen in aggregated hypertrophic ED1-positive cells, possibly microglial cells that have fused to create multinucleated giant cells. Occasionally, degradable nanowires with an apparently intact shape were found inside single, small ED1-positive cells. The biostable nanowires were found intact in microglia cells of both phenotypes described. Conclusion: The present study shows that the degradable nanowires remain at least partly in the brain over long time periods, i.e. 1 year; however, no obvious bio-safety issues for this degradable nanomaterial could be detected.</p>},
  articleno    = {64},
  author       = {Gällentoft, Lina and Pettersson, Lina M E and Danielsen, Nils and Schouenborg, Jens and Prinz, Christelle N. and Eriksson, Cecilia},
  keyword      = {Biocompatibility,Biomaterial,Brain,Foreign body reaction,Immunohistochemistry,Nanomedicine,Nanowires,Neural interfaces,Tissue responses},
  language     = {eng},
  month        = {08},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Journal of Nanobiotechnology},
  title        = {Impact of degradable nanowires on long-term brain tissue responses},
  url          = {http://dx.doi.org/10.1186/s12951-016-0216-7},
  volume       = {14},
  year         = {2016},
}