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Metabolic engineering of thermophilic bacteria for production of biotechnologically interesting compounds

Nordberg Karlsson, Eva LU ; Sardari, Roya R.R. LU ; Ron, Emanuel Y.C. LU ; Bjornsdottir, Snaedis H. ; Adalsteinsson, Bjorn T. ; Fridjonsson, Olafur H. and Hreggvidsson, Gudmundur O. (2020) In Life in Extreme Environments p.73-96
Abstract
Many thermophilic bacteria are efficient biomass degraders (producing polysaccharide degrading enzymes and utilizing a great variety of substrates, e.g. lignocellulosic polymers, pentoses, hexoses, as well sugar acids, and sugar alcohols). This makes them interesting organisms as potential cell factories in a circular bioeconomy. Lignocellulosic and marine macroalgal biomasses are regarded as sustainable biorefinery feedstocks for the production of energy carriers and platform and specialty chemicals, thereby meeting impending fossil fuel shortage and counteracting accumulation of greenhouse gasses. However, progress in using thermophilic bacteria that utilize these feedstocks as carbon sources has been hampered by the lack of suitable... (More)
Many thermophilic bacteria are efficient biomass degraders (producing polysaccharide degrading enzymes and utilizing a great variety of substrates, e.g. lignocellulosic polymers, pentoses, hexoses, as well sugar acids, and sugar alcohols). This makes them interesting organisms as potential cell factories in a circular bioeconomy. Lignocellulosic and marine macroalgal biomasses are regarded as sustainable biorefinery feedstocks for the production of energy carriers and platform and specialty chemicals, thereby meeting impending fossil fuel shortage and counteracting accumulation of greenhouse gasses. However, progress in using thermophilic bacteria that utilize these feedstocks as carbon sources has been hampered by the lack of suitable engineering tools to improve the production profiles of interesting target metabolites as specific synthetic production pathways need to be inserted/modified or existing pathways optimized by metabolic engineering. In this chapter, we review the progress on the use of thermophilic bacteria in metabolic engineering and the available engineering tools and give examples of species for which successful engineering has been accomplished. Today, the majority of thermophilic bacteria targeted for production of compounds of industrial interest by metabolic engineering belong to the phylum Firmicutes (e.g. Thermoanaerobacterium, Caldocellulosiruptor, Geobacillus, and Bacillus), taking advantage of anaerobic catabolic pathways producing organic acids and alcohols. However, there are additional and aerobic species gaining interest concerning biomass degradation and the ability of carbon dioxide fixation as well as production of molecules of interest, and some examples of this are also given. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
extremophiles, CRISPR-Cas, engineering
host publication
Biotechnological applications of extremophilic microorganisms
series title
Life in Extreme Environments
editor
Lee, Natuschka M.
pages
24 pages
publisher
De Gruyter
ISBN
978-3-11-042433-1
DOI
10.1515/9783110424331-003
language
English
LU publication?
yes
id
92270f40-c157-47ce-a1f4-d5da18b26e8c
date added to LUP
2020-12-08 20:28:13
date last changed
2020-12-11 02:25:49
@inbook{92270f40-c157-47ce-a1f4-d5da18b26e8c,
  abstract     = {Many thermophilic bacteria are efficient biomass degraders (producing polysaccharide degrading enzymes and utilizing a great variety of substrates, e.g. lignocellulosic polymers, pentoses, hexoses, as well sugar acids, and sugar alcohols). This makes them interesting organisms as potential cell factories in a circular bioeconomy. Lignocellulosic and marine macroalgal biomasses are regarded as sustainable biorefinery feedstocks for the production of energy carriers and platform and specialty chemicals, thereby meeting impending fossil fuel shortage and counteracting accumulation of greenhouse gasses. However, progress in using thermophilic bacteria that utilize these feedstocks as carbon sources has been hampered by the lack of suitable engineering tools to improve the production profiles of interesting target metabolites as specific synthetic production pathways need to be inserted/modified or existing pathways optimized by metabolic engineering. In this chapter, we review the progress on the use of thermophilic bacteria in metabolic engineering and the available engineering tools and give examples of species for which successful engineering has been accomplished. Today, the majority of thermophilic bacteria targeted for production of compounds of industrial interest by metabolic engineering belong to the phylum Firmicutes (e.g. Thermoanaerobacterium, Caldocellulosiruptor, Geobacillus, and Bacillus), taking advantage of anaerobic catabolic pathways producing organic acids and alcohols. However, there are additional and aerobic species gaining interest concerning biomass degradation and the ability of carbon dioxide fixation as well as production of molecules of interest, and some examples of this are also given.},
  author       = {Nordberg Karlsson, Eva and Sardari, Roya R.R. and Ron, Emanuel Y.C. and Bjornsdottir, Snaedis H. and Adalsteinsson, Bjorn T. and Fridjonsson, Olafur H. and Hreggvidsson, Gudmundur O.},
  booktitle    = {Biotechnological applications of extremophilic microorganisms},
  editor       = {Lee, Natuschka M.},
  isbn         = {978-3-11-042433-1},
  language     = {eng},
  pages        = {73--96},
  publisher    = {De Gruyter},
  series       = {Life in Extreme Environments},
  title        = {Metabolic engineering of thermophilic bacteria for production of biotechnologically interesting compounds},
  url          = {http://dx.doi.org/10.1515/9783110424331-003},
  doi          = {10.1515/9783110424331-003},
  year         = {2020},
}