Metabolic engineering of thermophilic bacteria for production of biotechnologically interesting compounds
(2020) In Life in Extreme Environments p.73-96- Abstract
- Many thermophilic bacteria are efficient biomass degraders (producing polysaccharide degrading enzymes and utilizing a great variety of substrates, e.g. lignocellulosic polymers, pentoses, hexoses, as well sugar acids, and sugar alcohols). This makes them interesting organisms as potential cell factories in a circular bioeconomy. Lignocellulosic and marine macroalgal biomasses are regarded as sustainable biorefinery feedstocks for the production of energy carriers and platform and specialty chemicals, thereby meeting impending fossil fuel shortage and counteracting accumulation of greenhouse gasses. However, progress in using thermophilic bacteria that utilize these feedstocks as carbon sources has been hampered by the lack of suitable... (More)
- Many thermophilic bacteria are efficient biomass degraders (producing polysaccharide degrading enzymes and utilizing a great variety of substrates, e.g. lignocellulosic polymers, pentoses, hexoses, as well sugar acids, and sugar alcohols). This makes them interesting organisms as potential cell factories in a circular bioeconomy. Lignocellulosic and marine macroalgal biomasses are regarded as sustainable biorefinery feedstocks for the production of energy carriers and platform and specialty chemicals, thereby meeting impending fossil fuel shortage and counteracting accumulation of greenhouse gasses. However, progress in using thermophilic bacteria that utilize these feedstocks as carbon sources has been hampered by the lack of suitable engineering tools to improve the production profiles of interesting target metabolites as specific synthetic production pathways need to be inserted/modified or existing pathways optimized by metabolic engineering. In this chapter, we review the progress on the use of thermophilic bacteria in metabolic engineering and the available engineering tools and give examples of species for which successful engineering has been accomplished. Today, the majority of thermophilic bacteria targeted for production of compounds of industrial interest by metabolic engineering belong to the phylum Firmicutes (e.g. Thermoanaerobacterium, Caldocellulosiruptor, Geobacillus, and Bacillus), taking advantage of anaerobic catabolic pathways producing organic acids and alcohols. However, there are additional and aerobic species gaining interest concerning biomass degradation and the ability of carbon dioxide fixation as well as production of molecules of interest, and some examples of this are also given. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/92270f40-c157-47ce-a1f4-d5da18b26e8c
- author
- Nordberg Karlsson, Eva LU ; Sardari, Roya R.R. LU ; Ron, Emanuel Y.C. LU ; Bjornsdottir, Snaedis H. ; Adalsteinsson, Bjorn T. ; Fridjonsson, Olafur H. and Hreggvidsson, Gudmundur O.
- organization
- publishing date
- 2020
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- extremophiles, CRISPR-Cas, engineering
- host publication
- Biotechnological applications of extremophilic microorganisms
- series title
- Life in Extreme Environments
- editor
- Lee, Natuschka M.
- pages
- 24 pages
- publisher
- De Gruyter
- external identifiers
-
- scopus:85109117545
- ISBN
- 978-3-11-042433-1
- DOI
- 10.1515/9783110424331-003
- language
- English
- LU publication?
- yes
- id
- 92270f40-c157-47ce-a1f4-d5da18b26e8c
- date added to LUP
- 2020-12-08 20:28:13
- date last changed
- 2022-12-07 04:06:34
@inbook{92270f40-c157-47ce-a1f4-d5da18b26e8c, abstract = {{Many thermophilic bacteria are efficient biomass degraders (producing polysaccharide degrading enzymes and utilizing a great variety of substrates, e.g. lignocellulosic polymers, pentoses, hexoses, as well sugar acids, and sugar alcohols). This makes them interesting organisms as potential cell factories in a circular bioeconomy. Lignocellulosic and marine macroalgal biomasses are regarded as sustainable biorefinery feedstocks for the production of energy carriers and platform and specialty chemicals, thereby meeting impending fossil fuel shortage and counteracting accumulation of greenhouse gasses. However, progress in using thermophilic bacteria that utilize these feedstocks as carbon sources has been hampered by the lack of suitable engineering tools to improve the production profiles of interesting target metabolites as specific synthetic production pathways need to be inserted/modified or existing pathways optimized by metabolic engineering. In this chapter, we review the progress on the use of thermophilic bacteria in metabolic engineering and the available engineering tools and give examples of species for which successful engineering has been accomplished. Today, the majority of thermophilic bacteria targeted for production of compounds of industrial interest by metabolic engineering belong to the phylum Firmicutes (e.g. Thermoanaerobacterium, Caldocellulosiruptor, Geobacillus, and Bacillus), taking advantage of anaerobic catabolic pathways producing organic acids and alcohols. However, there are additional and aerobic species gaining interest concerning biomass degradation and the ability of carbon dioxide fixation as well as production of molecules of interest, and some examples of this are also given.}}, author = {{Nordberg Karlsson, Eva and Sardari, Roya R.R. and Ron, Emanuel Y.C. and Bjornsdottir, Snaedis H. and Adalsteinsson, Bjorn T. and Fridjonsson, Olafur H. and Hreggvidsson, Gudmundur O.}}, booktitle = {{Biotechnological applications of extremophilic microorganisms}}, editor = {{Lee, Natuschka M.}}, isbn = {{978-3-11-042433-1}}, keywords = {{extremophiles; CRISPR-Cas; engineering}}, language = {{eng}}, pages = {{73--96}}, publisher = {{De Gruyter}}, series = {{Life in Extreme Environments}}, title = {{Metabolic engineering of thermophilic bacteria for production of biotechnologically interesting compounds}}, url = {{http://dx.doi.org/10.1515/9783110424331-003}}, doi = {{10.1515/9783110424331-003}}, year = {{2020}}, }