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A LCMS Metabolomic Workflow to Investigate Metabolic Patterns in Human Intestinal Cells Exposed to Hydrolyzed Crab Waste Materials

Ó Fearghail, Fionn ; Behan, Patrice ; Engström, Niklas LU and Scheers, Nathalie (2021) In Frontiers in Bioengineering and Biotechnology 9. p.1-14
Abstract

We have developed a LCMS metabolomic workflow to investigate metabolic patterns from human intestinal cells treated with simulated gastrointestinal-digested hydrolyzed crab waste materials. This workflow facilitates smart and reproducible comparisons of cell cultures exposed to different treatments. In this case the variable was the hydrolysis methods, also accounting for the GI digestion giving an output of direct correlation between cellular metabolic patterns caused by the treatments. In addition, we used the output from this workflow to select treatments for further evaluation of the Caco-2 cell response in terms of tentative anti-inflammatory activity in the hopes to find value in the crab waste materials to be used for food... (More)

We have developed a LCMS metabolomic workflow to investigate metabolic patterns from human intestinal cells treated with simulated gastrointestinal-digested hydrolyzed crab waste materials. This workflow facilitates smart and reproducible comparisons of cell cultures exposed to different treatments. In this case the variable was the hydrolysis methods, also accounting for the GI digestion giving an output of direct correlation between cellular metabolic patterns caused by the treatments. In addition, we used the output from this workflow to select treatments for further evaluation of the Caco-2 cell response in terms of tentative anti-inflammatory activity in the hopes to find value in the crab waste materials to be used for food products. As hypothesized, the treatment identified to change the cellular metabolomic pattern most readily, was also found to cause the greatest effect in the cells, although the response was pro-inflammatory rather than anti-inflammatory, it proves that changes in cellular metabolic patterns are useful predictors of bioactivity. We conclude that the developed workflow allows for cost effective, rapid sample preparation as well as accurate and repeatable LCMS analysis and introduces a data pipeline specifically for probe the novel metabolite patterns created as a means to assess the performing treatments.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Caco-2, cells, LCMS, metabolomic, workflow
in
Frontiers in Bioengineering and Biotechnology
volume
9
article number
629083
pages
1 - 14
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85102068915
  • pmid:33681165
ISSN
2296-4185
DOI
10.3389/fbioe.2021.629083
language
English
LU publication?
no
additional info
Funding Information: This work was funded under the EU Seventh Framework Programme ERA-NET Marine Biotechnology Grant Scheme (Grant agreement ID 604814). Funding was administrated in Ireland by the Marine Institute as the national funding agency and by Västra Götalandsregionen in Sweden (Grant RUN 2017-00164). This work was also supported by additional funding awarded by the Royal Society of Chemistry Researcher Mobility Grant. Funding Information: We would like to acknowledge Runar Gjerp Solstad (Nofima AS, Troms?, Norway), Yang Zhou and Katleen Raes (Ghent University, Belgium) and Johan Robbens (ILVO, Oostende, Belgium) for providing the hydrolyzed crab materials used in the study. Acknowledgments also to Otto Savolainen and the staff at the Chalmers Mass Spectrometry Infrastructure (Chalmers University of Technology, Gothenburg, Sweden) for assistance with instrumentation. We would also like to acknowledge Shokoufeh Karimi (Uppsala University, Sweden) for helping out with preliminary work with digestion/cell studies. Funding. This work was funded under the EU Seventh Framework Programme ERA-NET Marine Biotechnology Grant Scheme (Grant agreement ID 604814). Funding was administrated in Ireland by the Marine Institute as the national funding agency and by V?stra G?talandsregionen in Sweden (Grant RUN 2017-00164). This work was also supported by additional funding awarded by the Royal Society of Chemistry Researcher Mobility Grant. Publisher Copyright: © Copyright © 2021 Ó Fearghail, Behan, Engström and Scheers. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
id
925b436e-de6b-42c3-994a-82a8d5f177ee
date added to LUP
2021-09-08 10:11:07
date last changed
2024-02-04 02:34:15
@article{925b436e-de6b-42c3-994a-82a8d5f177ee,
  abstract     = {{<p>We have developed a LCMS metabolomic workflow to investigate metabolic patterns from human intestinal cells treated with simulated gastrointestinal-digested hydrolyzed crab waste materials. This workflow facilitates smart and reproducible comparisons of cell cultures exposed to different treatments. In this case the variable was the hydrolysis methods, also accounting for the GI digestion giving an output of direct correlation between cellular metabolic patterns caused by the treatments. In addition, we used the output from this workflow to select treatments for further evaluation of the Caco-2 cell response in terms of tentative anti-inflammatory activity in the hopes to find value in the crab waste materials to be used for food products. As hypothesized, the treatment identified to change the cellular metabolomic pattern most readily, was also found to cause the greatest effect in the cells, although the response was pro-inflammatory rather than anti-inflammatory, it proves that changes in cellular metabolic patterns are useful predictors of bioactivity. We conclude that the developed workflow allows for cost effective, rapid sample preparation as well as accurate and repeatable LCMS analysis and introduces a data pipeline specifically for probe the novel metabolite patterns created as a means to assess the performing treatments.</p>}},
  author       = {{Ó Fearghail, Fionn and Behan, Patrice and Engström, Niklas and Scheers, Nathalie}},
  issn         = {{2296-4185}},
  keywords     = {{Caco-2; cells; LCMS; metabolomic; workflow}},
  language     = {{eng}},
  pages        = {{1--14}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Bioengineering and Biotechnology}},
  title        = {{A LCMS Metabolomic Workflow to Investigate Metabolic Patterns in Human Intestinal Cells Exposed to Hydrolyzed Crab Waste Materials}},
  url          = {{http://dx.doi.org/10.3389/fbioe.2021.629083}},
  doi          = {{10.3389/fbioe.2021.629083}},
  volume       = {{9}},
  year         = {{2021}},
}