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Photodynamic therapy dosimetry using multiexcitation multiemission wavelength : toward real-time prediction of treatment outcome

Mousavi, Monirehalsadat LU ; Moriyama, Lilian Tan ; Grecco, Clovis ; Saito Nogueira, Marcelo ; Svanberg, Katarina LU ; Kurachi, Cristina and Andersson-Engels, Stefan LU (2020) In Journal of Biomedical Optics 25(6). p.1-14
Abstract

Evaluating the optical properties of biological tissues is needed to achieve accurate dosimetry during photodynamic therapy (PDT). Currently, accurate assessment of the photosensitizer (PS) concentration by fluorescence measurements during PDT is typically hindered by the lack of information about tissue optical properties. In the present work, a hand-held fiber-optic probe instrument monitoring fluorescence and reflectance is used for assessing blood volume, reduced scattering coefficient, and PS concentration facilitating accurate dosimetry for PDT. System validation was carried out on tissue phantoms using nonlinear least squares support machine regression analysis. It showed a high correlation coefficient (>0.99) in the... (More)

Evaluating the optical properties of biological tissues is needed to achieve accurate dosimetry during photodynamic therapy (PDT). Currently, accurate assessment of the photosensitizer (PS) concentration by fluorescence measurements during PDT is typically hindered by the lack of information about tissue optical properties. In the present work, a hand-held fiber-optic probe instrument monitoring fluorescence and reflectance is used for assessing blood volume, reduced scattering coefficient, and PS concentration facilitating accurate dosimetry for PDT. System validation was carried out on tissue phantoms using nonlinear least squares support machine regression analysis. It showed a high correlation coefficient (>0.99) in the prediction of the PS concentration upon a large variety of phantom optical properties. In vivo measurements were conducted in a PDT chlorine e6 dose escalating trial involving 36 male Swiss mice with Ehrlich solid tumors in which fluences of 5, 15, and 40  J cm  -  2 were delivered at two fluence rates (100 and 40  mW cm  -  2). Remarkably, quantitative measurement of fluorophore concentration was achieved in the in vivo experiment. Diffuse reflectance spectroscopy (DRS) system was also used to independently measure the physiological properties of the target tissues for result comparisons. Then, blood volume and scattering coefficient measured by the fiber-optic probe system were compared with the corresponding result measured by DRS and showed agreement. Additionally, tumor hemoglobin oxygen saturation was measured using the DRS system. Overall, the system is capable of assessing the implicit photodynamic dose to predict the PDT outcome.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biomedical optics, dosimetry, fiber-optic probe, fluorescence, in vivo measurement, optical properties, photodynamic therapy
in
Journal of Biomedical Optics
volume
25
issue
6
pages
14 pages
publisher
SPIE
external identifiers
  • scopus:85083041288
  • pmid:32246614
ISSN
1560-2281
DOI
10.1117/1.JBO.25.6.063812
language
English
LU publication?
yes
id
9309f714-a4fc-48de-bdf4-924be10c0231
date added to LUP
2020-05-20 11:13:06
date last changed
2024-04-17 09:28:40
@article{9309f714-a4fc-48de-bdf4-924be10c0231,
  abstract     = {{<p>Evaluating the optical properties of biological tissues is needed to achieve accurate dosimetry during photodynamic therapy (PDT). Currently, accurate assessment of the photosensitizer (PS) concentration by fluorescence measurements during PDT is typically hindered by the lack of information about tissue optical properties. In the present work, a hand-held fiber-optic probe instrument monitoring fluorescence and reflectance is used for assessing blood volume, reduced scattering coefficient, and PS concentration facilitating accurate dosimetry for PDT. System validation was carried out on tissue phantoms using nonlinear least squares support machine regression analysis. It showed a high correlation coefficient (&gt;0.99) in the prediction of the PS concentration upon a large variety of phantom optical properties. In vivo measurements were conducted in a PDT chlorine e6 dose escalating trial involving 36 male Swiss mice with Ehrlich solid tumors in which fluences of 5, 15, and 40  J cm  -  2 were delivered at two fluence rates (100 and 40  mW cm  -  2). Remarkably, quantitative measurement of fluorophore concentration was achieved in the in vivo experiment. Diffuse reflectance spectroscopy (DRS) system was also used to independently measure the physiological properties of the target tissues for result comparisons. Then, blood volume and scattering coefficient measured by the fiber-optic probe system were compared with the corresponding result measured by DRS and showed agreement. Additionally, tumor hemoglobin oxygen saturation was measured using the DRS system. Overall, the system is capable of assessing the implicit photodynamic dose to predict the PDT outcome.</p>}},
  author       = {{Mousavi, Monirehalsadat and Moriyama, Lilian Tan and Grecco, Clovis and Saito Nogueira, Marcelo and Svanberg, Katarina and Kurachi, Cristina and Andersson-Engels, Stefan}},
  issn         = {{1560-2281}},
  keywords     = {{biomedical optics; dosimetry; fiber-optic probe; fluorescence; in vivo measurement; optical properties; photodynamic therapy}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{1--14}},
  publisher    = {{SPIE}},
  series       = {{Journal of Biomedical Optics}},
  title        = {{Photodynamic therapy dosimetry using multiexcitation multiemission wavelength : toward real-time prediction of treatment outcome}},
  url          = {{http://dx.doi.org/10.1117/1.JBO.25.6.063812}},
  doi          = {{10.1117/1.JBO.25.6.063812}},
  volume       = {{25}},
  year         = {{2020}},
}