Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection
(2005) In Journal of Chromatography A 1080(2). p.132-139- Abstract
- A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique... (More)
- A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/233456
- author
- Surowiec, I ; Baena, JR ; Frank, J ; Laurell, Thomas LU ; Nilsson, Johan LU ; Trojanowicz, M and Lendl, B
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- flow through, HPLC, Raman microspectrometry, FTIR microscopy, microdispenser
- in
- Journal of Chromatography A
- volume
- 1080
- issue
- 2
- pages
- 132 - 139
- publisher
- Elsevier
- external identifiers
-
- wos:000230238600005
- pmid:16008051
- scopus:20444490460
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2005.04.082
- language
- English
- LU publication?
- yes
- id
- 9332c25f-f26c-4b60-8af3-4056a2f3b740 (old id 233456)
- date added to LUP
- 2016-04-01 16:18:52
- date last changed
- 2022-02-05 07:22:11
@article{9332c25f-f26c-4b60-8af3-4056a2f3b740, abstract = {{A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components.}}, author = {{Surowiec, I and Baena, JR and Frank, J and Laurell, Thomas and Nilsson, Johan and Trojanowicz, M and Lendl, B}}, issn = {{0021-9673}}, keywords = {{flow through; HPLC; Raman microspectrometry; FTIR microscopy; microdispenser}}, language = {{eng}}, number = {{2}}, pages = {{132--139}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection}}, url = {{http://dx.doi.org/10.1016/j.chroma.2005.04.082}}, doi = {{10.1016/j.chroma.2005.04.082}}, volume = {{1080}}, year = {{2005}}, }