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Bet v 1 homologues in strawberry identified as IgE-binding

Karlsson, Anne-Li; Alm, Rikard LU ; Ekstrand, Bo; Fjelkner-Modig, Stina; Schiött, Åsa LU ; Bengtsson, Ulf; Björk, Lars; Hjerno, Karin; Roepstorff, Peter and Emanuelsson, Cecilia LU (2004) In Allergy 59(12). p.1277-1284
Abstract
Background: No strawberry allergen has so far been identified and characterized.

Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh

and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.

Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These

two bands were excised and analysed by mass spectrometry showing the... (More)
Background: No strawberry allergen has so far been identified and characterized.

Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh

and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.

Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These

two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,

basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.

Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IgE, pollen, food allergy, allergens, clinical immunology
in
Allergy
volume
59
issue
12
pages
1277 - 1284
publisher
Wiley-Blackwell
external identifiers
  • wos:000224735800006
  • scopus:8644243740
ISSN
1398-9995
DOI
10.1111/j.1398-9995.2004.00585.x
language
English
LU publication?
yes
id
1d8e8a4a-5900-4512-9983-f2c73adf58c0 (old id 936892)
date added to LUP
2008-01-21 11:28:11
date last changed
2017-09-24 04:16:41
@article{1d8e8a4a-5900-4512-9983-f2c73adf58c0,
  abstract     = {Background: No strawberry allergen has so far been identified and characterized.<br/><br>
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh<br/><br>
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.<br/><br>
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These<br/><br>
two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,<br/><br>
basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.<br/><br>
Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.},
  author       = {Karlsson, Anne-Li and Alm, Rikard and Ekstrand, Bo and Fjelkner-Modig, Stina and Schiött, Åsa and Bengtsson, Ulf and Björk, Lars and Hjerno, Karin and Roepstorff, Peter and Emanuelsson, Cecilia},
  issn         = {1398-9995},
  keyword      = {IgE,pollen,food allergy,allergens,clinical immunology},
  language     = {eng},
  number       = {12},
  pages        = {1277--1284},
  publisher    = {Wiley-Blackwell},
  series       = {Allergy},
  title        = {Bet v 1 homologues in strawberry identified as IgE-binding},
  url          = {http://dx.doi.org/10.1111/j.1398-9995.2004.00585.x},
  volume       = {59},
  year         = {2004},
}