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DNA immobilization chemical interference due to aggregates study by Dip and Drop approach

Ajore, Ram LU ; Kumar, Rakesh; Kaur, Inderpreet; Sobti, R C and Bharadwaj, Lalit M (2007) In Journal of Biochemical and Biophysical Methods 70(5). p.85-779
Abstract

In the present manuscript, we report the studies and observations for chemical interference due to aggregates formation during covalent immobilization of thiolated lambda-DNA between gold microelectrodes. Dip and Drop approaches were employed to study DNA immobilization using thiolated oligos (oligoA 5' GGGCGGCGACCT 3' and oligoB 5' AGGTCGCCGCCC 3'). As a result of aggregation, less interference was observed in Dip approach as compared to Drop approach. Atomic Force Microscopy (AFM) analysis of piranha treated gold surface revealed 47.5% increase in height roughness, contributing in interference by creating active sites. Cyclic voltammetry (CV) studies ascertain the multitude of adsorption states existing in long strand of DNA on... (More)

In the present manuscript, we report the studies and observations for chemical interference due to aggregates formation during covalent immobilization of thiolated lambda-DNA between gold microelectrodes. Dip and Drop approaches were employed to study DNA immobilization using thiolated oligos (oligoA 5' GGGCGGCGACCT 3' and oligoB 5' AGGTCGCCGCCC 3'). As a result of aggregation, less interference was observed in Dip approach as compared to Drop approach. Atomic Force Microscopy (AFM) analysis of piranha treated gold surface revealed 47.5% increase in height roughness, contributing in interference by creating active sites. Cyclic voltammetry (CV) studies ascertain the multitude of adsorption states existing in long strand of DNA on surface. Surface coverage was found to be approximately 72% (1.35x10(10) molecules/cm(2)), and approximately 42% (7.89x10(9) molecules/ cm(2)) in Dip and Drop approach, respectively. Dip approach can be used as a measure to minimize interference due to aggregation.

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publishing date
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Contribution to journal
publication status
published
keywords
Adsorption, Bacteriophage lambda, Base Sequence, Biocompatible Materials, DNA, DNA, Viral, Electrochemistry, Gold, Macromolecular Substances, Microelectrodes, Oligodeoxyribonucleotides, Surface Properties, Thionucleotides, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Biochemical and Biophysical Methods
volume
70
issue
5
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:34347387294
ISSN
0165-022X
DOI
10.1016/j.jbbm.2007.05.006
language
English
LU publication?
no
id
93813e11-bab1-40dd-8b4c-bbd2d06ccc82
date added to LUP
2017-07-25 11:20:46
date last changed
2017-07-30 05:28:44
@article{93813e11-bab1-40dd-8b4c-bbd2d06ccc82,
  abstract     = {<p>In the present manuscript, we report the studies and observations for chemical interference due to aggregates formation during covalent immobilization of thiolated lambda-DNA between gold microelectrodes. Dip and Drop approaches were employed to study DNA immobilization using thiolated oligos (oligoA 5' GGGCGGCGACCT 3' and oligoB 5' AGGTCGCCGCCC 3'). As a result of aggregation, less interference was observed in Dip approach as compared to Drop approach. Atomic Force Microscopy (AFM) analysis of piranha treated gold surface revealed 47.5% increase in height roughness, contributing in interference by creating active sites. Cyclic voltammetry (CV) studies ascertain the multitude of adsorption states existing in long strand of DNA on surface. Surface coverage was found to be approximately 72% (1.35x10(10) molecules/cm(2)), and approximately 42% (7.89x10(9) molecules/ cm(2)) in Dip and Drop approach, respectively. Dip approach can be used as a measure to minimize interference due to aggregation.</p>},
  author       = {Ajore, Ram and Kumar, Rakesh and Kaur, Inderpreet and Sobti, R C and Bharadwaj, Lalit M},
  issn         = {0165-022X},
  keyword      = {Adsorption,Bacteriophage lambda,Base Sequence,Biocompatible Materials,DNA,DNA, Viral,Electrochemistry,Gold,Macromolecular Substances,Microelectrodes,Oligodeoxyribonucleotides,Surface Properties,Thionucleotides,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {08},
  number       = {5},
  pages        = {85--779},
  publisher    = {Elsevier},
  series       = {Journal of Biochemical and Biophysical Methods},
  title        = {DNA immobilization chemical interference due to aggregates study by Dip and Drop approach},
  url          = {http://dx.doi.org/10.1016/j.jbbm.2007.05.006},
  volume       = {70},
  year         = {2007},
}