Transcriptomic Analysis Reveals Differential Expression of Genes between Lung Capillary and Post Capillary Venules in Abdominal Sepsis
Rahman, Milladur LU
; Ding, Zhiyi
LU
; Rönnow, Carl-fredrik
LU
and Thorlacius, Henrik
LU
(2021)
In International Journal of Molecular Sciences
22(19).
p.1-19
- Abstract
- Lung endothelial cell dysfunction plays a central role in septic-induced lung injury. We hypothesized that endothelial cell subsets, capillary endothelial cells (capEC) and post capillary venules (PCV), might play different roles in regulating important pathophysiology in sepsis. In order to reveal global transcriptomic changes in endothelial cell subsets during sepsis, we induced sepsis in C57BL/6 mice by cecal ligation and puncture (CLP). We confirmed that CLP induced systemic and lung inflammation in our model. Endothelial cells (ECs) from lung capillary and PCV were isolated by cell sorting and transcriptomic changes were analyzed by bioinformatic tools. Our analysis revealed that lung capEC are transcriptionally different than PCV.... (More)
- Lung endothelial cell dysfunction plays a central role in septic-induced lung injury. We hypothesized that endothelial cell subsets, capillary endothelial cells (capEC) and post capillary venules (PCV), might play different roles in regulating important pathophysiology in sepsis. In order to reveal global transcriptomic changes in endothelial cell subsets during sepsis, we induced sepsis in C57BL/6 mice by cecal ligation and puncture (CLP). We confirmed that CLP induced systemic and lung inflammation in our model. Endothelial cells (ECs) from lung capillary and PCV were isolated by cell sorting and transcriptomic changes were analyzed by bioinformatic tools. Our analysis revealed that lung capEC are transcriptionally different than PCV. Comparison of top differentially expressed genes (DEGs) of capEC and PCV revealed that capEC responses are different than PCV during sepsis. It was found that capEC are more enriched with genes related to regulation of coagulation, vascular permeability, wound healing and lipid metabolic processes after sepsis. In contrast, PCV are more enriched with genes related to chemotaxis, cell–cell adhesion by integrins, chemokine biosynthesis, regulation of actin filament process and neutrophil homeostasis after sepsis. In addition, we predicted some transcription factor targets that regulate a significant number of DEGs in sepsis. We proposed that targeting certain DEGs or transcriptional factors would be useful in protecting against sepsis-induced lung damage (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/93c588c9-dd8e-4838-b1be-55c6201cc3aa
- author
- Rahman, Milladur
LU
; Ding, Zhiyi
LU
; Rönnow, Carl-fredrik
LU
and Thorlacius, Henrik
LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Molecular Sciences
- volume
- 22
- issue
- 19
- article number
- 10181
- pages
- 1 - 19
- publisher
- MDPI AG
- external identifiers
-
- scopus:85115365616
- pmid:34638535
- ISSN
- 1422-0067
- DOI
- 10.3390/ijms221910181
- language
- English
- LU publication?
- yes
- id
- 93c588c9-dd8e-4838-b1be-55c6201cc3aa
- date added to LUP
- 2021-10-06 13:37:06
- date last changed
- 2022-08-05 08:02:03
@article{93c588c9-dd8e-4838-b1be-55c6201cc3aa,
abstract = {{Lung endothelial cell dysfunction plays a central role in septic-induced lung injury. We hypothesized that endothelial cell subsets, capillary endothelial cells (capEC) and post capillary venules (PCV), might play different roles in regulating important pathophysiology in sepsis. In order to reveal global transcriptomic changes in endothelial cell subsets during sepsis, we induced sepsis in C57BL/6 mice by cecal ligation and puncture (CLP). We confirmed that CLP induced systemic and lung inflammation in our model. Endothelial cells (ECs) from lung capillary and PCV were isolated by cell sorting and transcriptomic changes were analyzed by bioinformatic tools. Our analysis revealed that lung capEC are transcriptionally different than PCV. Comparison of top differentially expressed genes (DEGs) of capEC and PCV revealed that capEC responses are different than PCV during sepsis. It was found that capEC are more enriched with genes related to regulation of coagulation, vascular permeability, wound healing and lipid metabolic processes after sepsis. In contrast, PCV are more enriched with genes related to chemotaxis, cell–cell adhesion by integrins, chemokine biosynthesis, regulation of actin filament process and neutrophil homeostasis after sepsis. In addition, we predicted some transcription factor targets that regulate a significant number of DEGs in sepsis. We proposed that targeting certain DEGs or transcriptional factors would be useful in protecting against sepsis-induced lung damage}},
author = {{Rahman, Milladur and Ding, Zhiyi and Rönnow, Carl-fredrik and Thorlacius, Henrik}},
issn = {{1422-0067}},
language = {{eng}},
number = {{19}},
pages = {{1--19}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Transcriptomic Analysis Reveals Differential Expression of Genes between Lung Capillary and Post Capillary Venules in Abdominal Sepsis}},
url = {{http://dx.doi.org/10.3390/ijms221910181}},
doi = {{10.3390/ijms221910181}},
volume = {{22}},
year = {{2021}},
}