Determination of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in human plasma and urine using liquid chromatography-electrospray tandem mass spectrometry
(2001) In Journal of Chromatography. B 761(1). p.107-113- Abstract
- A method for simultaneous determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) was developed. These compounds are metabolites from N-methyl-2-pyrrolidone (NMP), a powerful and widely used organic solvent. 5-HNMP and 2-HMSI were purified from plasma and urine by solid-phase extraction using Isolute ENV+ columns, and analysed by liquid chromatography coupled to a mass spectrometer fitted with an atmospheric pressure turbo ion spray ionisation interface in the positive ion mode. The method was validated for plasma and urine concentrations from 0.12 to 25 microg/ml. The recoveries for 5-HNMP and 2-HMSI in plasma were 99 and 98%, respectively, and in urine 111 and 106%, respectively. For 5-HNMP... (More)
- A method for simultaneous determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) was developed. These compounds are metabolites from N-methyl-2-pyrrolidone (NMP), a powerful and widely used organic solvent. 5-HNMP and 2-HMSI were purified from plasma and urine by solid-phase extraction using Isolute ENV+ columns, and analysed by liquid chromatography coupled to a mass spectrometer fitted with an atmospheric pressure turbo ion spray ionisation interface in the positive ion mode. The method was validated for plasma and urine concentrations from 0.12 to 25 microg/ml. The recoveries for 5-HNMP and 2-HMSI in plasma were 99 and 98%, respectively, and in urine 111 and 106%, respectively. For 5-HNMP and 2-HMSI, the within-day precision in plasma was 1-4 and 3-6%, respectively, and in urine 2-12 and 3-10%, respectively. The corresponding data for the between-day precision was 5 and 3-6%, respectively, and 4-6 and 7-8%, respectively. The detection limit for 5-HNMP was 4 ng/ml in plasma and 120 ng/ml in urine. For 2-HMSI, it was 5 ng/ml in plasma and 85 ng/ml in urine. The method is applicable for analysis of plasma and urine samples from workers exposed to NMP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1120673
- author
- Carnerup, Martin LU ; Åkesson, Bengt LU and Jönsson, Bo A LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5-Hydroxy-N-methyl-2-pyrrolidone, 2-Hydroxy-N-methylsuccinimide
- in
- Journal of Chromatography. B
- volume
- 761
- issue
- 1
- pages
- 107 - 113
- publisher
- Elsevier
- external identifiers
-
- pmid:11585124
- scopus:0035885241
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(01)00312-7
- language
- English
- LU publication?
- yes
- id
- 941bea92-203e-47b4-8b27-fd9b6d1d67cc (old id 1120673)
- date added to LUP
- 2016-04-01 15:49:29
- date last changed
- 2022-01-28 07:22:20
@article{941bea92-203e-47b4-8b27-fd9b6d1d67cc, abstract = {{A method for simultaneous determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) was developed. These compounds are metabolites from N-methyl-2-pyrrolidone (NMP), a powerful and widely used organic solvent. 5-HNMP and 2-HMSI were purified from plasma and urine by solid-phase extraction using Isolute ENV+ columns, and analysed by liquid chromatography coupled to a mass spectrometer fitted with an atmospheric pressure turbo ion spray ionisation interface in the positive ion mode. The method was validated for plasma and urine concentrations from 0.12 to 25 microg/ml. The recoveries for 5-HNMP and 2-HMSI in plasma were 99 and 98%, respectively, and in urine 111 and 106%, respectively. For 5-HNMP and 2-HMSI, the within-day precision in plasma was 1-4 and 3-6%, respectively, and in urine 2-12 and 3-10%, respectively. The corresponding data for the between-day precision was 5 and 3-6%, respectively, and 4-6 and 7-8%, respectively. The detection limit for 5-HNMP was 4 ng/ml in plasma and 120 ng/ml in urine. For 2-HMSI, it was 5 ng/ml in plasma and 85 ng/ml in urine. The method is applicable for analysis of plasma and urine samples from workers exposed to NMP.}}, author = {{Carnerup, Martin and Åkesson, Bengt and Jönsson, Bo A}}, issn = {{1387-2273}}, keywords = {{5-Hydroxy-N-methyl-2-pyrrolidone; 2-Hydroxy-N-methylsuccinimide}}, language = {{eng}}, number = {{1}}, pages = {{107--113}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Determination of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in human plasma and urine using liquid chromatography-electrospray tandem mass spectrometry}}, url = {{http://dx.doi.org/10.1016/S0378-4347(01)00312-7}}, doi = {{10.1016/S0378-4347(01)00312-7}}, volume = {{761}}, year = {{2001}}, }