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Paralysis of nematodes: shifts in the transcriptome of the nematode-trapping fungus Monacrosporium haptotylum during infection of Caenorhabditis elegans

Fekete, Csaba LU ; Tholander, Margareta LU ; Rajashekar, Balaji LU ; Ahrén, Dag LU ; Friman, Eva LU ; Johansson, Tomas LU and Tunlid, Anders LU (2008) In Environmental Microbiology 10(2). p.364-375
Abstract
The transcriptional response in the parasitic fungus Monacrosporium haptotylum and its nematode host Caenorhabditis elegans were analyzed during infection using cDNA microarrays. The array contained 2,684 fungal and 372 worm gene reporters. Dramatic shifts occurred in the transcriptome of M. haptotylum during the different stages of the infection. An initial transcriptional response was recorded after 1h of infection when the traps adhered to the cuticle, but before immobilization of the captured nematodes. Among the differentially expressed genes were two serine proteases (spr1 and spr2), and several homologues to genes known to be regulated in other pathogenic fungi. After 4 hours, when approximately 40 % of the nematodes were paralyzed,... (More)
The transcriptional response in the parasitic fungus Monacrosporium haptotylum and its nematode host Caenorhabditis elegans were analyzed during infection using cDNA microarrays. The array contained 2,684 fungal and 372 worm gene reporters. Dramatic shifts occurred in the transcriptome of M. haptotylum during the different stages of the infection. An initial transcriptional response was recorded after 1h of infection when the traps adhered to the cuticle, but before immobilization of the captured nematodes. Among the differentially expressed genes were two serine proteases (spr1 and spr2), and several homologues to genes known to be regulated in other pathogenic fungi. After 4 hours, when approximately 40 % of the nematodes were paralyzed, we identified an up-regulated cluster of 372 fungal genes which were not regulated during the other phases of the infection. This cohort contained a large proportion (79%) of genes that appear to be specific for M. haptotylum and closely related species. These genes were of two different classes; those translating into presumably functional peptides and those with no apparent protein coding potential (noncoding RNAs). Among the infection-induced C. elegans genes were those encoding antimicrobial peptides, protease inhibitors and lectins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Microbiology
volume
10
issue
2
pages
364 - 375
publisher
Wiley-Blackwell
external identifiers
  • wos:000252320800008
  • scopus:38049188323
ISSN
1462-2920
DOI
10.1111/j.1462-2920.2007.01457.x
language
English
LU publication?
yes
id
3661e641-7e71-4374-a3d6-58653556099e (old id 944032)
date added to LUP
2008-01-31 13:33:35
date last changed
2017-10-29 03:31:38
@article{3661e641-7e71-4374-a3d6-58653556099e,
  abstract     = {The transcriptional response in the parasitic fungus Monacrosporium haptotylum and its nematode host Caenorhabditis elegans were analyzed during infection using cDNA microarrays. The array contained 2,684 fungal and 372 worm gene reporters. Dramatic shifts occurred in the transcriptome of M. haptotylum during the different stages of the infection. An initial transcriptional response was recorded after 1h of infection when the traps adhered to the cuticle, but before immobilization of the captured nematodes. Among the differentially expressed genes were two serine proteases (spr1 and spr2), and several homologues to genes known to be regulated in other pathogenic fungi. After 4 hours, when approximately 40 % of the nematodes were paralyzed, we identified an up-regulated cluster of 372 fungal genes which were not regulated during the other phases of the infection. This cohort contained a large proportion (79%) of genes that appear to be specific for M. haptotylum and closely related species. These genes were of two different classes; those translating into presumably functional peptides and those with no apparent protein coding potential (noncoding RNAs). Among the infection-induced C. elegans genes were those encoding antimicrobial peptides, protease inhibitors and lectins.},
  author       = {Fekete, Csaba and Tholander, Margareta and Rajashekar, Balaji and Ahrén, Dag and Friman, Eva and Johansson, Tomas and Tunlid, Anders},
  issn         = {1462-2920},
  language     = {eng},
  number       = {2},
  pages        = {364--375},
  publisher    = {Wiley-Blackwell},
  series       = {Environmental Microbiology},
  title        = {Paralysis of nematodes: shifts in the transcriptome of the nematode-trapping fungus Monacrosporium haptotylum during infection of Caenorhabditis elegans},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2007.01457.x},
  volume       = {10},
  year         = {2008},
}