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Efficient spore synthesis in Bacillus subtilis depends on the CcdA protein.

Schiött, Torbjörn LU and Hederstedt, Lars LU (2000) In Journal of Bacteriology 182. p.2845-2854
Abstract
CcdA is known to be required for the synthesis of c-type cytochromes in Bacillus subtilis, but the exact function of this membrane protein is not known. We show that CcdA also plays a role in spore synthesis. The expression of ccdA and the two downstream genes yneI and yneJ was analyzed. There is a promoter for each gene, but there is only one transcription terminator, located after the yneJ gene. The promoter for ccdA was found to be weak and was active mainly during the transition from exponential growth to stationary phase. The promoters for yneI and yneJ were both active in the exponential growth phase. The levels of the CcdA and YneJ proteins in the membrane were consistent with the observed promoter activities. The ccdA promoter... (More)
CcdA is known to be required for the synthesis of c-type cytochromes in Bacillus subtilis, but the exact function of this membrane protein is not known. We show that CcdA also plays a role in spore synthesis. The expression of ccdA and the two downstream genes yneI and yneJ was analyzed. There is a promoter for each gene, but there is only one transcription terminator, located after the yneJ gene. The promoter for ccdA was found to be weak and was active mainly during the transition from exponential growth to stationary phase. The promoters for yneI and yneJ were both active in the exponential growth phase. The levels of the CcdA and YneJ proteins in the membrane were consistent with the observed promoter activities. The ccdA promoter activity was independent of whether the ccdA-yneI-yneJ gene products were absent or overproduced in the cell. It is shown that the four known cytochromes c in B. subtilis and the YneI and YneJ proteins are not required for sporulation. The combined data from analysis of sporulation-specific sigma factor activity, resistance properties of spores, and spore morphology indicate that CcdA deficiency affects stage V in sporulation. We conclude that CcdA, YneI, and YneJ are functionally unrelated proteins and that the role of CcdA in cytochrome c and spore synthesis probably relates to sulfhydryl redox chemistry on the outer surface of the cytoplasmic membrane. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
182
pages
2845 - 2854
publisher
American Society for Microbiology
external identifiers
  • scopus:0038755703
ISSN
0021-9193
DOI
10.1128/JB.182.10.2845-2854.2000
language
English
LU publication?
yes
id
94b43c74-b85c-4e1b-b48e-b7820e4ab59c
date added to LUP
2017-07-17 12:03:23
date last changed
2017-11-14 09:51:35
@article{94b43c74-b85c-4e1b-b48e-b7820e4ab59c,
  abstract     = {CcdA is known to be required for the synthesis of c-type cytochromes in Bacillus subtilis, but the exact function of this membrane protein is not known. We show that CcdA also plays a role in spore synthesis. The expression of ccdA and the two downstream genes yneI and yneJ was analyzed. There is a promoter for each gene, but there is only one transcription terminator, located after the yneJ gene. The promoter for ccdA was found to be weak and was active mainly during the transition from exponential growth to stationary phase. The promoters for yneI and yneJ were both active in the exponential growth phase. The levels of the CcdA and YneJ proteins in the membrane were consistent with the observed promoter activities. The ccdA promoter activity was independent of whether the ccdA-yneI-yneJ gene products were absent or overproduced in the cell. It is shown that the four known cytochromes c in B. subtilis and the YneI and YneJ proteins are not required for sporulation. The combined data from analysis of sporulation-specific sigma factor activity, resistance properties of spores, and spore morphology indicate that CcdA deficiency affects stage V in sporulation. We conclude that CcdA, YneI, and YneJ are functionally unrelated proteins and that the role of CcdA in cytochrome c and spore synthesis probably relates to sulfhydryl redox chemistry on the outer surface of the cytoplasmic membrane.},
  author       = {Schiött, Torbjörn and Hederstedt, Lars},
  issn         = {0021-9193},
  language     = {eng},
  pages        = {2845--2854},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Efficient spore synthesis in<em> Bacillus subtilis</em> depends on the CcdA protein.},
  url          = {http://dx.doi.org/10.1128/JB.182.10.2845-2854.2000},
  volume       = {182},
  year         = {2000},
}