Proteomic changes after fertilization and before first cleavage in rainbow trout Oncorhynchus mykiss
Niksirat, Hamid LU
; Levander, Fredrik
LU
; Kouba, Antonín
and James, Peter
LU
(2020)
In Aquaculture
520.
- Abstract
In most vertebrates, the development of a new individual starts with fertilization of the oocyte by the spermatozoon. A label-free quantification strategy was applied to investigate proteomic changes after fertilization and before first cleavage in the egg of rainbow trout. Samples were obtained from oocyte and fertilized eggs at 50, 200 min, and 15 h after fertilization at 4 °C. Significant changes were observed in the abundances of thirty-two proteins at different time points. The fertilization was accompanied by an upregulation of a coagulation factor protein which might be responsible for the construction of a layer for the protection of developing embryo. Upregulation of iron binding proteins such as ferritin and ceruloplasmin... (More)
In most vertebrates, the development of a new individual starts with fertilization of the oocyte by the spermatozoon. A label-free quantification strategy was applied to investigate proteomic changes after fertilization and before first cleavage in the egg of rainbow trout. Samples were obtained from oocyte and fertilized eggs at 50, 200 min, and 15 h after fertilization at 4 °C. Significant changes were observed in the abundances of thirty-two proteins at different time points. The fertilization was accompanied by an upregulation of a coagulation factor protein which might be responsible for the construction of a layer for the protection of developing embryo. Upregulation of iron binding proteins such as ferritin and ceruloplasmin could reflect the importance of this metal ion in the fertilization. The quantity of complement which is known as an immunological protein significantly increased during early stages. Upregulation of kinase could reflect the potentials of the fertilized egg for protein phosphorylation. Importin and vitellogenin proteins showed both down- and upregulation patterns. The timing of the upregulation of three types of actins may indicate their potential roles in fertilization and development of the fertilized egg. Upregulation of proteins such as Kinesin-like protein, Sororin etc. by 15 h may contribute to the cell preparation for the division in the upcoming first cleavage. In conclusion, insemination causes extensive changes in the abundances of proteins which have potentials to conduct fertilization and the cell preparation for the first cleavage. Data are available via ProteomeXchange with identifier PXD013329.
(Less)
- author
- Niksirat, Hamid
LU
; Levander, Fredrik
LU
; Kouba, Antonín
and James, Peter
LU
- organization
- publishing date
- 2020-04-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Egg, Fertilization, Gamete, Oocyte, Protein
- in
- Aquaculture
- volume
- 520
- article number
- 734951
- publisher
- Elsevier
- external identifiers
-
- scopus:85078558876
- ISSN
- 0044-8486
- DOI
- 10.1016/j.aquaculture.2020.734951
- language
- English
- LU publication?
- yes
- id
- 94f5e5c0-9e56-4cfa-b818-efadfde56448
- date added to LUP
- 2021-01-14 15:44:51
- date last changed
- 2023-11-20 21:08:40
@article{94f5e5c0-9e56-4cfa-b818-efadfde56448,
abstract = {{<p>In most vertebrates, the development of a new individual starts with fertilization of the oocyte by the spermatozoon. A label-free quantification strategy was applied to investigate proteomic changes after fertilization and before first cleavage in the egg of rainbow trout. Samples were obtained from oocyte and fertilized eggs at 50, 200 min, and 15 h after fertilization at 4 °C. Significant changes were observed in the abundances of thirty-two proteins at different time points. The fertilization was accompanied by an upregulation of a coagulation factor protein which might be responsible for the construction of a layer for the protection of developing embryo. Upregulation of iron binding proteins such as ferritin and ceruloplasmin could reflect the importance of this metal ion in the fertilization. The quantity of complement which is known as an immunological protein significantly increased during early stages. Upregulation of kinase could reflect the potentials of the fertilized egg for protein phosphorylation. Importin and vitellogenin proteins showed both down- and upregulation patterns. The timing of the upregulation of three types of actins may indicate their potential roles in fertilization and development of the fertilized egg. Upregulation of proteins such as Kinesin-like protein, Sororin etc. by 15 h may contribute to the cell preparation for the division in the upcoming first cleavage. In conclusion, insemination causes extensive changes in the abundances of proteins which have potentials to conduct fertilization and the cell preparation for the first cleavage. Data are available via ProteomeXchange with identifier PXD013329.</p>}},
author = {{Niksirat, Hamid and Levander, Fredrik and Kouba, Antonín and James, Peter}},
issn = {{0044-8486}},
keywords = {{Egg; Fertilization; Gamete; Oocyte; Protein}},
language = {{eng}},
month = {{04}},
publisher = {{Elsevier}},
series = {{Aquaculture}},
title = {{Proteomic changes after fertilization and before first cleavage in rainbow trout Oncorhynchus mykiss}},
url = {{http://dx.doi.org/10.1016/j.aquaculture.2020.734951}},
doi = {{10.1016/j.aquaculture.2020.734951}},
volume = {{520}},
year = {{2020}},
}