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Intercalation of cationic peptides within Laponite layered clay minerals in aqueous suspensions : The effect of stoichiometry and charge distance matching

Jansson, Maria LU ; Lenton, Samuel LU ; Plivelic, Tomás S. LU and Skepö, Marie LU (2019) In Journal of Colloid and Interface Science 557. p.767-776
Abstract

Clays can be synthesised to have specific functional properties, which have been exploited in a range of industrial processes. A key characteristic of clay is the presence of a negatively charged surface, surrounded by an oppositely charged rim. Because of that, clays are able to sequester cationic compounds resulting in the formation of ordered layered structures, known as tactoids. Recent research has highlighted the possibility of utilising clay as a drug delivery compound for cationic peptides. Here, we investigate the process of intercalation by using the highly cationic peptide deca-arginine, and the synthetic clay Laponite, in aqueous suspensions with 2.5 wt% Laponite, and varying peptide concentrations. Small-angle X-ray... (More)

Clays can be synthesised to have specific functional properties, which have been exploited in a range of industrial processes. A key characteristic of clay is the presence of a negatively charged surface, surrounded by an oppositely charged rim. Because of that, clays are able to sequester cationic compounds resulting in the formation of ordered layered structures, known as tactoids. Recent research has highlighted the possibility of utilising clay as a drug delivery compound for cationic peptides. Here, we investigate the process of intercalation by using the highly cationic peptide deca-arginine, and the synthetic clay Laponite, in aqueous suspensions with 2.5 wt% Laponite, and varying peptide concentrations. Small-angle X-ray scattering experiments show that tactoids are formed as a function of deca-arginine concentration in the dispersion, and for an excess of peptide, i.e. above a matched charge-ratio between the peptide and clay, the growth of the tactoids is limited, resulting in tactoidal dissolution. Zeta-potential measurements confirm that the observed dissolution is caused by overcharging of the platelets. By employing coarse-grained molecular dynamics simulations based on the continuum model, we are able to predict the tactoid formation, the growth, and the dissolution, in agreement with experimental results. We propose that the present simulation method can be a useful tool to tune peptide and clay characteristics to optimise and determine the extent of intercalation by cationic peptides of therapeutic interest.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cationic peptides, Clay, Deca-Arginine, Intercalation, Laponite, Molecular dynamics simulations, Nano-platelets, Small angle X-ray scattering, Tactoids, Zeta-potential
in
Journal of Colloid and Interface Science
volume
557
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85072712160
ISSN
0021-9797
DOI
10.1016/j.jcis.2019.09.055
language
English
LU publication?
yes
id
95fa7fcf-3a97-4cfe-ad89-36c13fc5be25
date added to LUP
2019-10-09 14:06:13
date last changed
2019-10-23 06:23:58
@article{95fa7fcf-3a97-4cfe-ad89-36c13fc5be25,
  abstract     = {<p>Clays can be synthesised to have specific functional properties, which have been exploited in a range of industrial processes. A key characteristic of clay is the presence of a negatively charged surface, surrounded by an oppositely charged rim. Because of that, clays are able to sequester cationic compounds resulting in the formation of ordered layered structures, known as tactoids. Recent research has highlighted the possibility of utilising clay as a drug delivery compound for cationic peptides. Here, we investigate the process of intercalation by using the highly cationic peptide deca-arginine, and the synthetic clay Laponite, in aqueous suspensions with 2.5 wt% Laponite, and varying peptide concentrations. Small-angle X-ray scattering experiments show that tactoids are formed as a function of deca-arginine concentration in the dispersion, and for an excess of peptide, i.e. above a matched charge-ratio between the peptide and clay, the growth of the tactoids is limited, resulting in tactoidal dissolution. Zeta-potential measurements confirm that the observed dissolution is caused by overcharging of the platelets. By employing coarse-grained molecular dynamics simulations based on the continuum model, we are able to predict the tactoid formation, the growth, and the dissolution, in agreement with experimental results. We propose that the present simulation method can be a useful tool to tune peptide and clay characteristics to optimise and determine the extent of intercalation by cationic peptides of therapeutic interest.</p>},
  author       = {Jansson, Maria and Lenton, Samuel and Plivelic, Tomás S. and Skepö, Marie},
  issn         = {0021-9797},
  keyword      = {Cationic peptides,Clay,Deca-Arginine,Intercalation,Laponite,Molecular dynamics simulations,Nano-platelets,Small angle X-ray scattering,Tactoids,Zeta-potential},
  language     = {eng},
  pages        = {767--776},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Intercalation of cationic peptides within Laponite layered clay minerals in aqueous suspensions : The effect of stoichiometry and charge distance matching},
  url          = {http://dx.doi.org/10.1016/j.jcis.2019.09.055},
  volume       = {557},
  year         = {2019},
}