Intercalation of cationic peptides within Laponite layered clay minerals in aqueous suspensions : The effect of stoichiometry and charge distance matching
(2019) In Journal of Colloid and Interface Science 557. p.767-776- Abstract
Clays can be synthesised to have specific functional properties, which have been exploited in a range of industrial processes. A key characteristic of clay is the presence of a negatively charged surface, surrounded by an oppositely charged rim. Because of that, clays are able to sequester cationic compounds resulting in the formation of ordered layered structures, known as tactoids. Recent research has highlighted the possibility of utilising clay as a drug delivery compound for cationic peptides. Here, we investigate the process of intercalation by using the highly cationic peptide deca-arginine, and the synthetic clay Laponite, in aqueous suspensions with 2.5 wt% Laponite, and varying peptide concentrations. Small-angle X-ray... (More)
Clays can be synthesised to have specific functional properties, which have been exploited in a range of industrial processes. A key characteristic of clay is the presence of a negatively charged surface, surrounded by an oppositely charged rim. Because of that, clays are able to sequester cationic compounds resulting in the formation of ordered layered structures, known as tactoids. Recent research has highlighted the possibility of utilising clay as a drug delivery compound for cationic peptides. Here, we investigate the process of intercalation by using the highly cationic peptide deca-arginine, and the synthetic clay Laponite, in aqueous suspensions with 2.5 wt% Laponite, and varying peptide concentrations. Small-angle X-ray scattering experiments show that tactoids are formed as a function of deca-arginine concentration in the dispersion, and for an excess of peptide, i.e. above a matched charge-ratio between the peptide and clay, the growth of the tactoids is limited, resulting in tactoidal dissolution. Zeta-potential measurements confirm that the observed dissolution is caused by overcharging of the platelets. By employing coarse-grained molecular dynamics simulations based on the continuum model, we are able to predict the tactoid formation, the growth, and the dissolution, in agreement with experimental results. We propose that the present simulation method can be a useful tool to tune peptide and clay characteristics to optimise and determine the extent of intercalation by cationic peptides of therapeutic interest.
(Less)
- author
- Jansson, Maria LU ; Lenton, Samuel LU ; Plivelic, Tomás S. LU and Skepö, Marie LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cationic peptides, Clay, Deca-Arginine, Intercalation, Laponite, Molecular dynamics simulations, Nano-platelets, Small angle X-ray scattering, Tactoids, Zeta-potential
- in
- Journal of Colloid and Interface Science
- volume
- 557
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:31569056
- scopus:85072712160
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2019.09.055
- language
- English
- LU publication?
- yes
- id
- 95fa7fcf-3a97-4cfe-ad89-36c13fc5be25
- date added to LUP
- 2019-10-09 14:06:13
- date last changed
- 2024-04-16 21:47:25
@article{95fa7fcf-3a97-4cfe-ad89-36c13fc5be25,
abstract = {{<p>Clays can be synthesised to have specific functional properties, which have been exploited in a range of industrial processes. A key characteristic of clay is the presence of a negatively charged surface, surrounded by an oppositely charged rim. Because of that, clays are able to sequester cationic compounds resulting in the formation of ordered layered structures, known as tactoids. Recent research has highlighted the possibility of utilising clay as a drug delivery compound for cationic peptides. Here, we investigate the process of intercalation by using the highly cationic peptide deca-arginine, and the synthetic clay Laponite, in aqueous suspensions with 2.5 wt% Laponite, and varying peptide concentrations. Small-angle X-ray scattering experiments show that tactoids are formed as a function of deca-arginine concentration in the dispersion, and for an excess of peptide, i.e. above a matched charge-ratio between the peptide and clay, the growth of the tactoids is limited, resulting in tactoidal dissolution. Zeta-potential measurements confirm that the observed dissolution is caused by overcharging of the platelets. By employing coarse-grained molecular dynamics simulations based on the continuum model, we are able to predict the tactoid formation, the growth, and the dissolution, in agreement with experimental results. We propose that the present simulation method can be a useful tool to tune peptide and clay characteristics to optimise and determine the extent of intercalation by cationic peptides of therapeutic interest.</p>}},
author = {{Jansson, Maria and Lenton, Samuel and Plivelic, Tomás S. and Skepö, Marie}},
issn = {{0021-9797}},
keywords = {{Cationic peptides; Clay; Deca-Arginine; Intercalation; Laponite; Molecular dynamics simulations; Nano-platelets; Small angle X-ray scattering; Tactoids; Zeta-potential}},
language = {{eng}},
pages = {{767--776}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Intercalation of cationic peptides within Laponite layered clay minerals in aqueous suspensions : The effect of stoichiometry and charge distance matching}},
url = {{http://dx.doi.org/10.1016/j.jcis.2019.09.055}},
doi = {{10.1016/j.jcis.2019.09.055}},
volume = {{557}},
year = {{2019}},
}