RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.
Baudet, Aurelie LU
; Karlsson, Christine
LU
; Safaee Talkhoncheh, Mehrnaz
LU
; Galeev, Roman
LU
; Magnusson, Mattias
LU
and Larsson, Jonas
LU
(2012)
In Blood
119(26).
p.6255-6258
- Abstract
- We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2609154
- author
- Baudet, Aurelie
LU
; Karlsson, Christine
LU
; Safaee Talkhoncheh, Mehrnaz
LU
; Galeev, Roman
LU
; Magnusson, Mattias
LU
and Larsson, Jonas
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 119
- issue
- 26
- pages
- 6255 - 6258
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000307400700016
- pmid:22555972
- scopus:84863520113
- pmid:22555972
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2012-01-403949
- language
- English
- LU publication?
- yes
- id
- 9617e44f-9812-407c-a5df-bb3fd8241d2b (old id 2609154)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22555972?dopt=Abstract
- date added to LUP
- 2016-04-04 08:21:52
- date last changed
- 2025-10-14 10:36:18
@article{9617e44f-9812-407c-a5df-bb3fd8241d2b,
abstract = {{We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.}},
author = {{Baudet, Aurelie and Karlsson, Christine and Safaee Talkhoncheh, Mehrnaz and Galeev, Roman and Magnusson, Mattias and Larsson, Jonas}},
issn = {{1528-0020}},
language = {{eng}},
number = {{26}},
pages = {{6255--6258}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.}},
url = {{http://dx.doi.org/10.1182/blood-2012-01-403949}},
doi = {{10.1182/blood-2012-01-403949}},
volume = {{119}},
year = {{2012}},
}