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Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells

Ding, W Q ; Larsson, Christer LU ; Simonsson, Per LU and Alling, Christer LU (1997) In Brain Research. Molecular Brain Research 46(1-2). p.77-84
Abstract
The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol... (More)
The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muscarinic acetylcholine receptor, Ethanol, c-fos expression, Protein kinase C, Neuroblastoma SH-SY5Y cell
in
Brain Research. Molecular Brain Research
volume
46
issue
1-2
pages
77 - 84
publisher
Elsevier
external identifiers
  • pmid:9191080
  • scopus:0030911415
ISSN
0169-328X
DOI
10.1016/S0169-328X(96)00273-2
language
English
LU publication?
yes
id
962d2d5d-8b3d-4304-a616-596ad4d14622 (old id 1111179)
date added to LUP
2016-04-01 16:01:12
date last changed
2022-01-28 08:44:20
@article{962d2d5d-8b3d-4304-a616-596ad4d14622,
  abstract     = {{The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure.}},
  author       = {{Ding, W Q and Larsson, Christer and Simonsson, Per and Alling, Christer}},
  issn         = {{0169-328X}},
  keywords     = {{Muscarinic acetylcholine receptor; Ethanol; c-fos expression; Protein kinase C; Neuroblastoma SH-SY5Y cell}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{77--84}},
  publisher    = {{Elsevier}},
  series       = {{Brain Research. Molecular Brain Research}},
  title        = {{Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells}},
  url          = {{http://dx.doi.org/10.1016/S0169-328X(96)00273-2}},
  doi          = {{10.1016/S0169-328X(96)00273-2}},
  volume       = {{46}},
  year         = {{1997}},
}