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Encapsulation of DNA in Macroscopic and Nanosized Calcium Alginate Gel Particles

Machado, Alexandra LU ; Lundberg, Dan LU ; Ribeiro, Antonio J.; Veiga, Francisco J.; Miguel, Maria G.; Lindman, Björn LU and Olsson, Ulf LU (2013) In Langmuir 29(51). p.15926-15935
Abstract
Calcium alginate beads, which are biodegradable and biocompatible, have been widely employed as delivery matrices for biomacromolecules. In the present work, the feasibility of encapsulation of DNA (which is used as a model biomacromolecule) in calcium alginate nanobeads (sub-200 nm size), prepared using a recently developed protocol based on the phase inversion temperature (PIT) emulsification method [Machado et al. Langmuir 2012, 28, 4131-4141], was assessed. The properties of the nanobeads were compared to those of the corresponding macroscopic (millimeter sized) calcium alginate beads. It was found that DNA, representing a relatively stiff and highly charged polyanion (thus like-charged to alginate), could be efficiently encapsulated... (More)
Calcium alginate beads, which are biodegradable and biocompatible, have been widely employed as delivery matrices for biomacromolecules. In the present work, the feasibility of encapsulation of DNA (which is used as a model biomacromolecule) in calcium alginate nanobeads (sub-200 nm size), prepared using a recently developed protocol based on the phase inversion temperature (PIT) emulsification method [Machado et al. Langmuir 2012, 28, 4131-4141], was assessed. The properties of the nanobeads were compared to those of the corresponding macroscopic (millimeter sized) calcium alginate beads. It was found that DNA, representing a relatively stiff and highly charged polyanion (thus like-charged to alginate), could be efficiently encapsulated in both nanosized and macroscopic beads, with encapsulation yields in the range of 77-99%. Complete release of DNA from the beads could be accomplished on dissolution of the gel by addition of a calcium-chelating agent. Importantly, the DNA was not denatured or fragmented during the preparation and collection of the nanobeads, which are good indicators of the mildness of the preparation protocol used. The calcium alginate nanobeads prepared by the herein utilized protocol thus show good potential to be used as carriers of sensitive biomacromolecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
29
issue
51
pages
15926 - 15935
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000329137000024
  • scopus:84896506290
ISSN
0743-7463
DOI
10.1021/la4032927
language
English
LU publication?
yes
id
964b31ef-21c0-4f62-a703-969ccfbfeb52 (old id 4255937)
date added to LUP
2014-02-10 13:33:20
date last changed
2019-09-04 01:22:14
@article{964b31ef-21c0-4f62-a703-969ccfbfeb52,
  abstract     = {Calcium alginate beads, which are biodegradable and biocompatible, have been widely employed as delivery matrices for biomacromolecules. In the present work, the feasibility of encapsulation of DNA (which is used as a model biomacromolecule) in calcium alginate nanobeads (sub-200 nm size), prepared using a recently developed protocol based on the phase inversion temperature (PIT) emulsification method [Machado et al. Langmuir 2012, 28, 4131-4141], was assessed. The properties of the nanobeads were compared to those of the corresponding macroscopic (millimeter sized) calcium alginate beads. It was found that DNA, representing a relatively stiff and highly charged polyanion (thus like-charged to alginate), could be efficiently encapsulated in both nanosized and macroscopic beads, with encapsulation yields in the range of 77-99%. Complete release of DNA from the beads could be accomplished on dissolution of the gel by addition of a calcium-chelating agent. Importantly, the DNA was not denatured or fragmented during the preparation and collection of the nanobeads, which are good indicators of the mildness of the preparation protocol used. The calcium alginate nanobeads prepared by the herein utilized protocol thus show good potential to be used as carriers of sensitive biomacromolecules.},
  author       = {Machado, Alexandra and Lundberg, Dan and Ribeiro, Antonio J. and Veiga, Francisco J. and Miguel, Maria G. and Lindman, Björn and Olsson, Ulf},
  issn         = {0743-7463},
  language     = {eng},
  number       = {51},
  pages        = {15926--15935},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Langmuir},
  title        = {Encapsulation of DNA in Macroscopic and Nanosized Calcium Alginate Gel Particles},
  url          = {http://dx.doi.org/10.1021/la4032927},
  volume       = {29},
  year         = {2013},
}