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Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells

Gomzikova, Marina O.; Zhuravleva, Margarita N.; Miftakhova, Regina R.; Arkhipova, Svetlana S.; Evtugin, Vladimir G.; Khaiboullina, Svetlana F.; Kiyasov, Andrey P.; Persson, Jenny L. LU ; Mongan, Nigel P. LU and Pestell, Richard G., et al. (2017) In Oncotarget 8(41). p.70496-70507
Abstract

Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared... (More)

Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Angiogenesis, Cell-free therapy, Cytochalasin B-induced membrane vesicles, Extracellular vesicles, Membrane vesicles
in
Oncotarget
volume
8
issue
41
pages
12 pages
publisher
Impact Journals, LLC
external identifiers
  • scopus:85030257003
  • wos:000411153300103
ISSN
1949-2553
DOI
10.18632/oncotarget.19723
language
English
LU publication?
yes
id
96789369-97d4-42b6-a6d5-4f03868b8a87
date added to LUP
2017-11-06 15:59:57
date last changed
2018-01-16 13:25:47
@article{96789369-97d4-42b6-a6d5-4f03868b8a87,
  abstract     = {<p>Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.</p>},
  author       = {Gomzikova, Marina O. and Zhuravleva, Margarita N. and Miftakhova, Regina R. and Arkhipova, Svetlana S. and Evtugin, Vladimir G. and Khaiboullina, Svetlana F. and Kiyasov, Andrey P. and Persson, Jenny L. and Mongan, Nigel P. and Pestell, Richard G. and Rizvanov, Albert},
  issn         = {1949-2553},
  keyword      = {Angiogenesis,Cell-free therapy,Cytochalasin B-induced membrane vesicles,Extracellular vesicles,Membrane vesicles},
  language     = {eng},
  number       = {41},
  pages        = {70496--70507},
  publisher    = {Impact Journals, LLC},
  series       = {Oncotarget},
  title        = {Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells},
  url          = {http://dx.doi.org/10.18632/oncotarget.19723},
  volume       = {8},
  year         = {2017},
}